Detailed information NTG0307429

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: DV947_RS07235
• Genome accession: NZ_CP031545
• Coordinates: 1370334..1371029 (-)
• Length: 231 a.a.
• NCBI ID: WP_014608614.1
• Uniprot ID: A0AAU9H5B0
• Organism: Streptococcus thermophilus strain ST109
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1365334..1376029

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DV947_RS07220 (DV947_07225)	-	1366145..1366690 (-)	546	WP_022096992.1	GNAT family N-acetyltransferase	-
DV947_RS07225 (DV947_07230)	-	1366756..1368018 (-)	1263	WP_022096991.1	UDP-N-acetylglucosamine 1-carboxyvinyltransferase	-
DV947_RS07230 (DV947_07235)	-	1368104..1370344 (-)	2241	WP_014608613.1	DNA internalization-related competence protein ComEC/Rec2	-
DV947_RS07235 (DV947_07240)	comEA	1370334..1371029 (-)	696	WP_014608614.1	helix-hairpin-helix domain-containing protein	Machinery gene
DV947_RS07240 (DV947_07245)	-	1371136..1371890 (-)	755	Protein_1342	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
DV947_RS10590	-	1372379..1372611 (+)	233	Protein_1343	polysaccharide deacetylase family protein	-
DV947_RS10320 (DV947_07255)	-	1372666..1372953 (+)	288	WP_096811598.1	peptidoglycan GlcNAc deacetylase	-
DV947_RS07255 (DV947_07260)	-	1373003..1373767 (+)	765	WP_116920332.1	tRNA1(Val) (adenine(37)-N6)-methyltransferase	-
DV947_RS07260 (DV947_07265)	-	1373771..1374040 (+)	270	WP_011226404.1	GIY-YIG nuclease family protein	-
DV947_RS07270 (DV947_07275)	-	1374655..1375917 (+)	1263	WP_205412155.1	ISL3-like element ISSth1 family transposase	-

Sequence

Protein

• Length: 231 a.a.
• Molecular weight: 24602.52 Da
• Isoelectric Point: 4.5661

>NTDB_id=307429 DV947_RS07235 WP_014608614.1 1370334..1371029(-) (comEA) [Streptococcus thermophilus strain ST109]
MKEKILAYVKDNRLFVSVIAVLMVIFCFFLWMTCGAGNSMEAETSYTDVTALSTSSSKQSSQSLSEASSQSKTEGSEKVK
SKVTVDVKGAVVNPGVYTLKEGARVTDVIQEAGGMTEDADAKSVNLAASLSDEEVIYVANKDENVSVLDQTSTGQVSDKG
GQAVSKDGKINLNTATSEQLQTISGIGAKRTEDIIAYRESHGGFQSVDDLKNVSGIGDKTLDKIRESLYVA

Nucleotide

• Length: 696 bp

>NTDB_id=307429 DV947_RS07235 WP_014608614.1 1370334..1371029(-) (comEA) [Streptococcus thermophilus strain ST109]
GTGAAGGAAAAGATTCTAGCCTATGTCAAAGATAATCGTCTGTTTGTGAGTGTCATCGCTGTACTGATGGTGATTTTTTG
CTTCTTTTTATGGATGACTTGTGGTGCCGGCAACAGCATGGAGGCGGAGACGTCTTATACAGATGTGACAGCTTTGTCAA
CGTCTTCATCCAAACAAAGTTCACAGTCTCTTTCTGAGGCGTCTTCCCAGTCAAAGACCGAAGGAAGTGAAAAAGTTAAG
TCAAAAGTAACGGTAGATGTTAAGGGGGCTGTGGTCAATCCAGGTGTCTATACGCTAAAGGAAGGCGCTAGGGTGACAGA
TGTCATTCAGGAAGCTGGAGGAATGACAGAAGATGCAGACGCTAAGAGTGTTAACTTAGCCGCAAGCTTGTCAGATGAAG
AGGTTATTTATGTAGCCAATAAAGATGAAAATGTTTCTGTCCTTGATCAAACAAGTACTGGTCAGGTCTCTGACAAAGGA
GGGCAGGCTGTATCTAAGGATGGTAAAATTAACTTAAATACAGCAACCTCAGAGCAGTTGCAAACCATTTCTGGAATTGG
AGCTAAGCGGACAGAGGATATCATTGCCTATCGTGAAAGTCATGGCGGCTTTCAGTCCGTAGATGACTTGAAAAATGTCT
CAGGAATTGGTGATAAAACATTAGATAAAATCAGAGAGTCCCTCTATGTGGCTTAA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (167-228)

• SLBB domain (SLBB) (85-138)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Streptococcus thermophilus LMD-9	98.268	100	0.983
comEA/celA/cilE	Streptococcus mitis SK321	40.175	99.134	0.398
comEA/celA/cilE	Streptococcus pneumoniae D39	38.596	98.701	0.381
comEA/celA/cilE	Streptococcus pneumoniae R6	38.596	98.701	0.381
comEA/celA/cilE	Streptococcus pneumoniae Rx1	38.596	98.701	0.381
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	38.596	98.701	0.381
comEA/celA/cilE	Streptococcus mitis NCTC 12261	37.281	98.701	0.368