Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   DXX82_RS07605 Genome accession   NZ_CP031477
Coordinates   1551270..1551812 (+) Length   180 a.a.
NCBI ID   WP_115912350.1    Uniprot ID   -
Organism   Haemophilus parainfluenzae strain M27794     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1546270..1556812
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DXX82_RS07585 - 1546919..1547839 (-) 921 WP_065286311.1 ATP-binding cassette domain-containing protein -
  DXX82_RS07590 - 1547839..1548720 (-) 882 WP_049357006.1 metal ABC transporter substrate-binding protein -
  DXX82_RS07595 - 1548875..1549750 (-) 876 WP_115912346.1 serine hydrolase -
  DXX82_RS07600 - 1550052..1551200 (+) 1149 WP_115912347.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  DXX82_RS07605 pilF/pilF2 1551270..1551812 (+) 543 WP_115912350.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  DXX82_RS07610 - 1551886..1552914 (+) 1029 WP_005695589.1 RodZ family helix-turn-helix domain-containing protein -
  DXX82_RS07615 ispG 1552933..1554039 (+) 1107 WP_111406806.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  DXX82_RS07620 hisS 1554049..1555320 (+) 1272 WP_049379993.1 histidine--tRNA ligase -
  DXX82_RS07625 - 1555339..1555953 (+) 615 WP_115912352.1 YfgM family protein -
  DXX82_RS07630 - 1556005..1556448 (+) 444 WP_115912354.1 isoprenylcysteine carboxylmethyltransferase family protein -

Sequence

Protein


Download         Length: 180 a.a.        Molecular weight: 20363.98 Da        Isoelectric Point: 6.0886

>NTDB_id=307144 DXX82_RS07605 WP_115912350.1 1551270..1551812(+) (pilF/pilF2) [Haemophilus parainfluenzae strain M27794]
MKLIPINILSAVIFPFVFSACVSQSSVDFDKQQAAKARVELALGYLQQNDFVQAKLNLDKALEHDERYYLVHSALAHFYQ
LQGDTEKAKQAYLQAIKLDDKQGDVYNNFGAFLCGQGEFEQAYSQFNAALAAPNYYHQADTYENMALCAFAGKQTDVYQQ
ALDKLRQVDPSRSEKLRSLK

Nucleotide


Download         Length: 543 bp        

>NTDB_id=307144 DXX82_RS07605 WP_115912350.1 1551270..1551812(+) (pilF/pilF2) [Haemophilus parainfluenzae strain M27794]
ATGAAATTAATCCCAATTAACATTCTAAGTGCGGTCATTTTTCCTTTTGTTTTTTCTGCCTGTGTCTCGCAGTCTTCCGT
TGACTTTGATAAACAACAAGCAGCAAAAGCGCGAGTTGAACTGGCATTAGGCTATCTTCAACAAAATGATTTTGTTCAAG
CAAAACTCAATTTAGATAAAGCCCTTGAGCATGATGAACGATATTATCTTGTGCACTCAGCACTTGCACATTTTTATCAA
TTACAAGGCGATACGGAAAAAGCGAAGCAAGCTTATTTACAGGCGATTAAGTTAGATGATAAGCAAGGCGATGTGTATAA
CAACTTCGGTGCTTTTTTATGTGGGCAAGGTGAGTTTGAACAAGCTTATTCACAATTTAATGCGGCACTCGCTGCGCCGA
ATTATTACCATCAAGCCGATACTTACGAAAACATGGCGCTTTGTGCTTTCGCCGGAAAACAAACCGATGTTTATCAACAG
GCATTGGATAAATTGCGTCAAGTTGATCCTTCTAGATCGGAAAAGCTCCGTTCACTCAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(74-101)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

65.116

95.556

0.622
  pilF Glaesserella parasuis strain SC1401

48.588

98.333

0.478

Multiple sequence alignment