Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EYD11_RS18855 Genome accession   NZ_CP036177
Coordinates   3877893..3878333 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain WCHEC025970     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3872893..3883333
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EYD11_RS18835 (EYD11_18830) aroP 3873891..3875264 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  EYD11_RS18840 (EYD11_18835) ampE 3875307..3876161 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  EYD11_RS18845 (EYD11_18840) ampD 3876158..3876709 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EYD11_RS18850 (EYD11_18845) nadC 3876797..3877690 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EYD11_RS18855 (EYD11_18850) pilA 3877893..3878333 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  EYD11_RS18860 (EYD11_18855) pilB 3878343..3879728 (+) 1386 WP_001025146.1 type II secretion system protein GspE Machinery gene
  EYD11_RS18865 (EYD11_18860) hofC 3879718..3880920 (+) 1203 WP_000157266.1 protein transport protein HofC -
  EYD11_RS18870 (EYD11_18865) guaC 3880955..3881998 (-) 1044 WP_001217338.1 GMP reductase -
  EYD11_RS26775 - 3882154..3882198 (-) 45 WP_120795372.1 protein YacM -
  EYD11_RS18875 (EYD11_18870) coaE 3882223..3882843 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=306771 EYD11_RS18855 WP_000360904.1 3877893..3878333(+) (pilA) [Escherichia coli strain WCHEC025970]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=306771 EYD11_RS18855 WP_000360904.1 3877893..3878333(+) (pilA) [Escherichia coli strain WCHEC025970]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363