Detailed information NTG0306695

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: DXX91_RS12505
• Genome accession: NZ_CP031424
• Coordinates: 2542668..2543282 (-)
• Length: 204 a.a.
• NCBI ID: WP_043867297.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain B-4
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2537668..2548282

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DXX91_RS12480	rpsT	2537968..2538234 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
DXX91_RS12485	holA	2538251..2539292 (-)	1042	Protein_2404	DNA polymerase III subunit delta	-
DXX91_RS19845	-	2539332..2539486 (-)	155	Protein_2405	hypothetical protein	-
DXX91_RS12490	-	2539527..2539661 (+)	135	WP_003152870.1	YqzM family protein	-
DXX91_RS12495	comEC	2539680..2542031 (-)	2352	WP_115996550.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
DXX91_RS12500	-	2542032..2542601 (-)	570	WP_003152868.1	ComE operon protein 2	-
DXX91_RS12505	comEA	2542668..2543282 (-)	615	WP_043867297.1	helix-hairpin-helix domain-containing protein	Machinery gene
DXX91_RS12510	comER	2543341..2544162 (+)	822	WP_115996552.1	late competence protein ComER	-
DXX91_RS12515	-	2544231..2544968 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
DXX91_RS12520	rsfS	2544965..2545321 (-)	357	WP_003152864.1	ribosome silencing factor	-
DXX91_RS12525	yqeK	2545318..2545899 (-)	582	WP_003152863.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
DXX91_RS12530	-	2545889..2546458 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
DXX91_RS12535	yhbY	2546469..2546759 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
DXX91_RS12540	aroE	2546753..2547595 (-)	843	WP_115996555.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22178.37 Da
• Isoelectric Point: 6.9685

>NTDB_id=306695 DXX91_RS12505 WP_043867297.1 2542668..2543282(-) (comEA) [Bacillus velezensis strain B-4]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEGNHPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=306695 DXX91_RS12505 WP_043867297.1 2542668..2543282(-) (comEA) [Bacillus velezensis strain B-4]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGGGAATCATCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363