Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EXT04_RS18480 Genome accession   NZ_CP035944
Coordinates   3781463..3781903 (+) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain AR24.2b     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3776463..3786903
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXT04_RS18460 (EXT04_18485) aroP 3777461..3778834 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  EXT04_RS18465 (EXT04_18490) ampE 3778877..3779731 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  EXT04_RS18470 (EXT04_18495) ampD 3779728..3780279 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EXT04_RS18475 (EXT04_18500) nadC 3780367..3781260 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EXT04_RS18480 (EXT04_18505) pilA 3781463..3781903 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  EXT04_RS18485 (EXT04_18510) pilB 3781913..3783298 (+) 1386 WP_001025173.1 type II secretion system protein GspE Machinery gene
  EXT04_RS18490 (EXT04_18515) hofC 3783288..3784490 (+) 1203 WP_000157227.1 protein transport protein HofC -
  EXT04_RS18495 (EXT04_18520) guaC 3784525..3785568 (-) 1044 WP_001217342.1 GMP reductase -
  EXT04_RS22835 - 3785724..3785768 (-) 45 WP_120795372.1 protein YacM -
  EXT04_RS18500 (EXT04_18525) coaE 3785793..3786413 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=306613 EXT04_RS18480 WP_000360895.1 3781463..3781903(+) (pilA) [Escherichia coli strain AR24.2b]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=306613 EXT04_RS18480 WP_000360895.1 3781463..3781903(+) (pilA) [Escherichia coli strain AR24.2b]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment