Detailed information    

insolico Bioinformatically predicted

Overview

Name   comL   Type   Machinery gene
Locus tag   EW999_RS21815 Genome accession   NZ_CP035870
Coordinates   4454656..4455393 (-) Length   245 a.a.
NCBI ID   WP_000197686.1    Uniprot ID   P0AC03
Organism   Escherichia coli strain E2528-C1     
Function   DNA binding (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4449656..4460393
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW999_RS21785 yfiL 4449814..4450179 (-) 366 WP_000976004.1 DUF2799 domain-containing protein -
  EW999_RS21790 aroF 4450389..4451459 (+) 1071 WP_001168037.1 3-deoxy-7-phosphoheptulonate synthase AroF -
  EW999_RS21795 tyrA 4451470..4452591 (+) 1122 WP_000225221.1 bifunctional chorismate mutase/prephenate dehydrogenase -
  EW999_RS21800 pheA 4452634..4453794 (-) 1161 WP_000200099.1 bifunctional chorismate mutase/prephenate dehydratase -
  EW999_RS21805 pheL 4453893..4453940 (-) 48 WP_010723158.1 phe operon leader peptide -
  EW999_RS21810 raiA 4454044..4454385 (-) 342 WP_000178456.1 ribosome-associated translation inhibitor RaiA -
  EW999_RS21815 comL 4454656..4455393 (-) 738 WP_000197686.1 outer membrane protein assembly factor BamD Machinery gene
  EW999_RS21820 rluD 4455528..4456508 (+) 981 WP_000079100.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  EW999_RS21825 yfiH 4456505..4457236 (+) 732 WP_000040169.1 purine nucleoside phosphorylase YfiH -
  EW999_RS21830 clpC 4457366..4459939 (+) 2574 WP_001235102.1 ATP-dependent chaperone ClpB Regulator

Sequence

Protein


Download         Length: 245 a.a.        Molecular weight: 27829.40 Da        Isoelectric Point: 6.4874

>NTDB_id=306180 EW999_RS21815 WP_000197686.1 4454656..4455393(-) (comL) [Escherichia coli strain E2528-C1]
MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQDGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYY
KNADLPLAQAAIDRFIRLNPTHPNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLMENAYRQMQMNAQAEKVAKIIAA
NSSNT

Nucleotide


Download         Length: 738 bp        

>NTDB_id=306180 EW999_RS21815 WP_000197686.1 4454656..4455393(-) (comL) [Escherichia coli strain E2528-C1]
ATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGT
ACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGC
AACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTAT
AAAAACGCCGATTTGCCGTTAGCACAGGCTGCCATCGATCGTTTTATTCGCCTTAACCCGACCCATCCGAATATCGATTA
TGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGCGTCGATCGTAGCG
ATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACC
ACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGA
ACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATG
CGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCA
AACAGCAGCAATACATAA

Domains

Predicted by InterProScan.

Outer membrane lipoprotein (YfiO)

(28-236)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P0AC03

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comL Neisseria meningitidis MC58

38.525

99.592

0.384
  comL Neisseria gonorrhoeae MS11

37.705

99.592

0.376

Multiple sequence alignment