Detailed information NTG0030610

Bioinformatically predicted

Overview

• Name: comEC/celB
• Type: Machinery gene
• Locus tag: SPCG_RS04845
• Genome accession: NC_010582
• Coordinates: 911456..913696 (+)
• Length: 746 a.a.
• NCBI ID: WP_000942394.1
• Uniprot ID: A0A166WJ67
• Organism: Streptococcus pneumoniae CGSP14
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 906456..918696

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SPCG_RS13015	-	906802..907592 (+)	791	Protein_916	IS5 family transposase	-
SPCG_RS04815 (SPCG_0925)	-	907775..908275 (+)	501	WP_000566988.1	GNAT family N-acetyltransferase	-
SPCG_RS04820 (SPCG_0926)	-	908278..908604 (+)	327	Protein_918	TfoX/Sxy family protein	-
SPCG_RS12290 (SPCG_0928)	ald	908909..910008 (-)	1100	Protein_919	alanine dehydrogenase	-
SPCG_RS04835 (SPCG_0929)	-	910185..910754 (+)	570	WP_000443750.1	GNAT family N-acetyltransferase	-
SPCG_RS04840 (SPCG_0930)	comEA/celA/cilE	910822..911472 (+)	651	WP_000387344.1	helix-hairpin-helix domain-containing protein	Machinery gene
SPCG_RS04845 (SPCG_0931)	comEC/celB	911456..913696 (+)	2241	WP_000942394.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
SPCG_RS04850 (SPCG_0932)	-	913875..914063 (+)	189	WP_001808514.1	hypothetical protein	-
SPCG_RS04855 (SPCG_0933)	-	914096..914675 (+)	580	Protein_924	ATP-binding cassette domain-containing protein	-
SPCG_RS04860 (SPCG_0934)	-	914679..915863 (+)	1185	WP_000655960.1	ABC transporter permease	-
SPCG_RS04865 (SPCG_0935)	infC	916174..916704 (+)	531	WP_000848180.1	translation initiation factor IF-3	-
SPCG_RS04870 (SPCG_0936)	rpmI	916737..916937 (+)	201	WP_001125943.1	50S ribosomal protein L35	-
SPCG_RS04875 (SPCG_0937)	rplT	916989..917348 (+)	360	WP_000124836.1	50S ribosomal protein L20	-
SPCG_RS04880 (SPCG_0938)	-	917406..917786 (+)	381	WP_000157154.1	VOC family protein	-

Sequence

Protein

• Length: 746 a.a.
• Molecular weight: 84444.00 Da
• Isoelectric Point: 9.6202

>NTDB_id=30610 SPCG_RS04845 WP_000942394.1 911456..913696(+) (comEC/celB) [Streptococcus pneumoniae CGSP14]
MLQWIKNFSIPLIYLSFLLLWLYYAIFSASYLALLGFVFLLVCLFIQFPWKSAGKVLIICGIFGFWFVFQNWQQSQASQN
LADSVERVRILPDTIKVNGDSLSFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
LKTQGIYQTLNIKTIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMGNYMTGLLLGHLDTDFEEMNELYSSLGIIHL
FALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPFSLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIV
MPNFFLTAGGVLSCAYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDLVFLPLLSI
LFVLSFLYPVIQLNFIFEWLEGIIRLVSQVARRPLVFGQPNAWLLILLLISLALVYDLRKNIKGLTVLSLLITGLFFLTK
YPLENEITMLDVGQGESIFLRDVTGKTILIDVGGKAESYKKIKKWQEKMTTSNAQRTLIPYLKSRGVAKIDQLILTNTDK
EHVGDLSEMTKAFHVGEILVSKDSLKQKEFVAELQATQTKVRSMIVGENLPIFGSQLEVLSPRKMGDGGHDDTLVLYGKF
LDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSSSPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGIN
SKVYRTDQQGAIRFKGLDSWKIESVR

Nucleotide

• Length: 2241 bp

>NTDB_id=30610 SPCG_RS04845 WP_000942394.1 911456..913696(+) (comEC/celB) [Streptococcus pneumoniae CGSP14]
ATGTTACAGTGGATTAAGAATTTCTCTATTCCCCTAATTTACCTGAGTTTTCTATTACTTTGGCTTTATTACGCTATTTT
CTCAGCATCTTATCTTGCTTTGTTGGGCTTTGTTTTTCTGCTAGTCTGTCTCTTTATCCAATTTCCGTGGAAATCTGCTG
GTAAAGTTCTAATAATTTGCGGAATCTTTGGATTTTGGTTTGTTTTTCAAAATTGGCAACAGAGTCAAGCGAGTCAAAAT
CTGGCGGATTCTGTTGAAAGGGTACGGATTTTGCCTGATACTATTAAGGTTAATGGTGATAGTCTATCCTTTCGTGGCAA
GTCTAACGGTCGTGCTTTCCAAGTCTATTATAAACTCCAGTCCGAGGAGGAGAAAGAAGCCTTTCAAGCTTTAACTGACC
TGCATGAGATAGGACTAGAAGGGAAGCTTTCGGAGCCAGAAGGGCAGAGAAATTTTGGTGGCTTTAATTACCAAGCCTAT
CTGAAGACTCAGGGAATTTACCAGACTCTCAATATCAAAACAATCCAGTCACTTCAAAAGATTGGCAGTTGGGATATAGG
AGAAAACTTGTCCAGTTTACGTCGAAAGGCTGTGGTTTGGATTAAGACGCACTTTCCAGACCCTATGGGCAATTACATGA
CAGGACTCTTGCTGGGACATCTGGACACCGACTTTGAGGAGATGAATGAGCTTTATTCCAGTCTAGGAATTATCCACCTC
TTTGCCCTATCTGGCATGCAGGTAGGTTTTTTCATGAATGGATTTAAGAAACTTCTCTTGCGATTGGGCTTGACCCAAGA
AAAGTTGAAATGGCTGACTTATCCCTTTTCCCTTATCTATGCGGGACTAACTGGATTTTCAGCATCGGTTATTCGCAGTC
TCTTGCAAAAGCTACTGGCTCAACATGGGGTTAAGGGCTTGGATAATTTTGCCTTGACGGTGCTTGTCCTCTTTATTGTC
ATGCCAAACTTTTTCTTGACAGCAGGAGGAGTCTTGTCCTGCGCTTATGCTTTTATCCTGACCATGACCAGCAAAGAAGG
GGAGGGGCTCAAGGCTGTTACTAGTGAAAGTCTAGTCATCTCCTTGGGCATATTGCCCATTCTATCCTTCTATTTTGCGG
AATTTCAACCTTGGTCTATCCTTTTGACCTTTGTCTTTTCCTTTCTTTTTGACTTGGTCTTCTTACCGCTCTTGTCTATC
TTATTTGTCCTTTCCTTTCTCTATCCAGTCATTCAGCTGAACTTTATCTTTGAATGGTTAGAGGGCATTATTCGCTTGGT
CTCGCAGGTGGCAAGGAGACCACTTGTCTTTGGTCAACCCAACGCATGGCTTTTAATCTTATTGTTAATTTCCTTGGCTT
TGGTCTATGATTTGAGGAAAAACATTAAAGGATTAACAGTATTGAGTTTATTGATTACAGGTCTCTTTTTCCTTACCAAG
TATCCACTGGAAAATGAAATCACCATGCTGGATGTGGGGCAAGGAGAAAGTATTTTCCTACGGGATGTAACTGGGAAAAC
CATTCTCATAGATGTAGGTGGTAAGGCAGAATCTTATAAGAAAATCAAAAAATGGCAAGAAAAGATGACGACCAGCAATG
CCCAGCGAACCTTGATTCCCTATCTCAAAAGTCGAGGAGTAGCTAAGATTGACCAGCTAATTTTGACTAACACGGACAAG
GAGCATGTTGGAGATTTGTCAGAGATGACCAAGGCTTTCCATGTAGGGGAGATTCTAGTATCAAAAGACAGTCTGAAACA
GAAGGAATTTGTGGCAGAACTACAGGCGACTCAAACAAAGGTGCGTAGTATGATAGTAGGGGAGAACTTGCCCATTTTTG
GAAGTCAGTTAGAAGTTCTATCTCCAAGGAAAATGGGAGATGGAGGACACGATGATACCCTAGTTCTGTATGGGAAATTC
TTGGATAAGCAATTTCTCTTCACGGGAAATTTGGAGGAGAAAGGAGAGAAGGACTTGCTGAAGCACTATCCAGACTTGAA
AGTAAATGTTTTGAAAGCTAGCCAACATGGCAATAAAAAATCATCAAGTCCAGCCTTTCTAGAAAAACTCAAACCAGAGC
TTACTCTTATCTCAGTTGGAAAGAGCAATCGAATGAAACTCCCCCATCAGGAAACATTGACACGACTGGAAGGTATCAAT
AGCAAAGTTTATCGAACTGACCAGCAAGGAGCTATACGTTTTAAGGGGTTGGATAGTTGGAAAATCGAAAGTGTTCGATA
G

Domains

Predicted by InterproScan.

• Competence protein (Competence) (216-458)

• Domain of unknown function (DUF4131) (DUF4131) (30-176)

• Metallo-beta-lactamase superfamily (Lactamase_B) (492-693)

3D structure

• AlphaFold DB: A0A166WJ67

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC/celB	Streptococcus pneumoniae TIGR4	100	100	1
comEC/celB	Streptococcus pneumoniae Rx1	97.587	100	0.976
comEC/celB	Streptococcus pneumoniae D39	97.587	100	0.976
comEC/celB	Streptococcus pneumoniae R6	97.587	100	0.976
comEC/celB	Streptococcus mitis SK321	92.225	100	0.922
comEC/celB	Streptococcus mitis NCTC 12261	91.812	99.866	0.917
comEC	Lactococcus lactis subsp. cremoris KW2	44.399	99.33	0.441