Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   DV178_RS02520 Genome accession   NZ_CP031335
Coordinates   427589..428449 (+) Length   286 a.a.
NCBI ID   WP_002241067.1    Uniprot ID   A1KVV2
Organism   Neisseria meningitidis strain M25964     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 422589..433449
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DV178_RS02495 dapD 423099..423920 (-) 822 WP_002220281.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -
  DV178_RS02500 - 423930..424160 (-) 231 WP_025462060.1 hypothetical protein -
  DV178_RS02505 pgi 424130..425773 (-) 1644 WP_002247959.1 glucose-6-phosphate isomerase -
  DV178_RS02515 pilG 426283..427515 (+) 1233 WP_002216405.1 type 4 pilus assembly protein PilG Machinery gene
  DV178_RS02520 pilD 427589..428449 (+) 861 WP_002241067.1 prepilin peptidase Machinery gene
  DV178_RS02525 coaE 428451..429083 (+) 633 WP_002216402.1 dephospho-CoA kinase -
  DV178_RS02530 - 429076..429285 (+) 210 WP_002216401.1 DNA gyrase inhibitor YacG -
  DV178_RS02535 pilF 429370..431046 (-) 1677 WP_002221967.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DV178_RS02555 - 431538..431984 (-) 447 WP_002220271.1 DUF441 domain-containing protein -
  DV178_RS02560 ispB 431989..432963 (-) 975 WP_002247958.1 octaprenyl diphosphate synthase -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 31331.48 Da        Isoelectric Point: 9.1920

>NTDB_id=306010 DV178_RS02520 WP_002241067.1 427589..428449(+) (pilD) [Neisseria meningitidis strain M25964]
MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVFAKKHLNLPLTEKESRTFNLMKPDSCCPKCRVPIRAW
QNIPIVSYLLLRGKCASCQTKISIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGEIGMGNGDFKLIAALGAWLGISALPVLIFVSSLIGLV
AAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRAVNWWLTHPVL

Nucleotide


Download         Length: 861 bp        

>NTDB_id=306010 DV178_RS02520 WP_002241067.1 427589..428449(+) (pilD) [Neisseria meningitidis strain M25964]
ATGTCTGATTTGTCTGTATTGTCGCCGTTTGCCGTGCCTTTGGCAGCGGTGTTCGGGCTGCTGGTCGGCAGTTTCTTAAA
CGTCGTCATTTACCGCGTGCCGGTCATGATGGAACGCGGCTGGACGGTATTTGCCAAAAAACATTTAAACCTGCCGCTGA
CCGAAAAGGAAAGCCGTACCTTCAACCTGATGAAACCGGATTCCTGCTGTCCCAAATGCCGCGTGCCGATACGCGCGTGG
CAGAACATCCCGATTGTCAGCTACCTGCTCCTGCGCGGCAAATGCGCTTCCTGCCAAACCAAAATCAGCATACGTTATCC
CTTAATCGAGCTGCTGACCGGCGTATTATTCGGGCTGGTCGCCTGGCAATACGGCTGGTCTTGGATTACATTGGGCGGTT
TGATACTGACCGCGTTTCTGATTTCCCTGACCTTTATCGATGCGGACACCCAATACCTGCCCGACTCGATGACACTGCCC
TTGATTTGGCTGGGGCTGATATTTAATTTGGACGGCGGCTTCGTGCCTTTGCAGTCTGCCGTTTTAGGTGCGGTTGCCGG
CTATGGTTCGATATGGCTTTTATGCGCGGTATATAAACTGATTAAAGGCGAAATCGGTATGGGCAACGGAGATTTCAAAC
TGATTGCCGCATTGGGCGCGTGGCTCGGCATATCCGCATTGCCCGTGCTGATTTTTGTTTCCTCGCTGATCGGTTTGGTC
GCGGCAATCGTTATGCGCGTCGCCAAGGGGCAGCATTTTGCCTTCGGCCCCGCACTGACAGTTTCGGGCTGGATAATTTT
TACGGCAAACGATTCCGTATGGCGGGCGGTCAACTGGTGGCTGACCCATCCGGTGCTGTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(134-242)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-124)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A1KVV2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

96.14

99.65

0.958
  pilD Vibrio cholerae strain A1552

44.444

100

0.448
  pilD Acinetobacter baumannii D1279779

46.97

92.308

0.434
  pilD Acinetobacter nosocomialis M2

46.97

92.308

0.434
  pilD Vibrio campbellii strain DS40M4

44.565

96.503

0.43

Multiple sequence alignment