Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   DV166_RS04325 Genome accession   NZ_CP031334
Coordinates   726998..728263 (+) Length   421 a.a.
NCBI ID   WP_115436978.1    Uniprot ID   -
Organism   Neisseria meningitidis strain M22293     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 721998..733263
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DV166_RS04300 - 722727..723728 (+) 1002 WP_002223261.1 thiamine ABC transporter substrate-binding protein -
  DV166_RS04305 - 723822..724670 (+) 849 WP_002215243.1 mechanosensitive ion channel family protein -
  DV166_RS04310 - 724697..725182 (+) 486 WP_002215245.1 CinA family protein -
  DV166_RS04315 msrAB 725284..726852 (-) 1569 WP_002236293.1 bifunctional peptide-methionine (S)-S-oxide reductase MsrA/peptide-methionine (R)-S-oxide reductase MsrB -
  DV166_RS04325 pilA 726998..728263 (+) 1266 WP_115436978.1 signal recognition particle-docking protein FtsY Machinery gene
  DV166_RS04335 - 729267..729551 (+) 285 WP_002216166.1 GIY-YIG nuclease family protein -
  DV166_RS14770 pilC 730624..730755 (+) 132 WP_306808038.1 hypothetical protein Machinery gene

Sequence

Protein


Download         Length: 421 a.a.        Molecular weight: 45243.58 Da        Isoelectric Point: 4.5585

>NTDB_id=305969 DV166_RS04325 WP_115436978.1 726998..728263(+) (pilA) [Neisseria meningitidis strain M22293]
MFSFFRRKKKQETPAPEEAQVQETAAKAESEVTQIVENIKEEVESLTESVKGQVESAVETVSGAVEQVKETVAEMLSEAE
EAAEKAAEQVEAAKEAIAETVGEAVGQVQEAVATTEEHKLGWVARLKQGLTKSRDKMAKSLAGVFGGGQIDEDLYEELET
VLITSDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPETKEPFVIMLAGINGAGKTTSIGK
LAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGERNNVTVISQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHL
MEEIKKVKRVLQKAMPDAPHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRPVPVRYIGV
GEGIDDLRPFDARAFVDALLD

Nucleotide


Download         Length: 1266 bp        

>NTDB_id=305969 DV166_RS04325 WP_115436978.1 726998..728263(+) (pilA) [Neisseria meningitidis strain M22293]
ATGTTCAGCTTCTTCCGTCGCAAGAAAAAACAGGAAACGCCGGCTCCCGAGGAGGCTCAAGTTCAGGAAACTGCAGCAAA
AGCAGAATCCGAAGTTACTCAAATAGTTGAAAATATTAAAGAAGAAGTCGAATCTTTAACGGAAAGCGTCAAAGGGCAGG
TCGAATCGGCTGTTGAAACCGTCAGCGGTGCGGTTGAACAGGTAAAGGAAACCGTTGCCGAGATGCTGTCTGAAGCAGAG
GAAGCGGCGGAAAAAGCAGCGGAACAAGTCGAAGCGGCAAAAGAAGCCATTGCCGAAACCGTCGGCGAGGCTGTCGGGCA
AGTTCAAGAAGCCGTTGCAACAACTGAAGAACACAAGCTCGGTTGGGTGGCGCGTTTGAAACAAGGTTTGACCAAATCGC
GCGACAAAATGGCGAAATCGCTGGCGGGCGTGTTCGGCGGCGGACAAATCGACGAAGATTTGTACGAAGAGCTGGAAACC
GTGCTGATTACCAGCGACATGGGCATGGAAGCCACCGAATACCTGATGAAAGACGTGCGCGACCGCGTCAGCCTCAAAGG
GCTGAAAGACGGCAACGAATTGCGCGGCGCGTTGAAAGAAGCCTTGTACGACCTGATTAAGCCTCTGGAGAAACCGCTGG
TTTTGCCCGAAACCAAAGAGCCGTTTGTCATCATGCTTGCCGGCATCAACGGCGCGGGCAAAACCACGTCTATCGGCAAA
CTCGCCAAATATTTCCAAGCGCAGGGCAAATCCGTATTGCTGGCGGCAGGCGATACTTTCCGTGCCGCCGCGCGTGAGCA
GCTTCAAGCTTGGGGCGAGCGCAACAACGTAACCGTGATTTCGCAAACCACGGGCGATTCCGCCGCCGTGTGCTTCGATG
CCGTCCAAGCCGCCAAAGCGCGCGGCATCGACATTGTGCTGGCCGACACCGCCGGCCGCCTGCCCACGCAGCTTCACTTG
ATGGAAGAAATCAAAAAAGTGAAACGCGTGCTGCAAAAAGCCATGCCCGACGCGCCGCACGAAATCATCGTCGTGCTTGA
TGCCAACATCGGGCAAAACGCCGTCAACCAAGTCAAAGCCTTTGACGACGCATTAGGGCTGACGGGGCTTATCGTTACCA
AACTCGACGGCACGGCAAAAGGCGGCATCCTCGCCGCGCTTGCCTCCGACCGCCCCGTCCCCGTCCGCTATATCGGCGTG
GGCGAAGGCATAGACGATTTGCGCCCGTTTGACGCGCGCGCGTTTGTGGACGCACTGCTGGATTGA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(221-420)

SRP54-type protein, helical bundle domain (SRP54_N)

(136-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

94.062

100

0.941

Multiple sequence alignment