Detailed information NTG0305892

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: EXE31_RS13210
• Genome accession: NZ_CP035855
• Coordinates: 2708759..2709739 (-)
• Length: 326 a.a.
• NCBI ID: WP_001295381.1
• Uniprot ID: -
• Organism: Escherichia coli TW11681
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2703759..2714739

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EXE31_RS13180	yggI	2704414..2704911 (+)	498	WP_000858396.1	SprT family zinc-dependent metalloprotease	-
EXE31_RS13185	endA	2705006..2705713 (+)	708	WP_000286511.1	deoxyribonuclease I	-
EXE31_RS13190	rsmE	2705793..2706524 (+)	732	WP_001222508.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
EXE31_RS13195	gshB	2706537..2707487 (+)	951	WP_000593273.1	glutathione synthase	-
EXE31_RS13200	yqgE	2707596..2708159 (+)	564	WP_001053178.1	YqgE/AlgH family protein	-
EXE31_RS13205	ruvX	2708159..2708575 (+)	417	WP_000017106.1	Holliday junction resolvase RuvX	-
EXE31_RS26300	-	2708582..2708710 (-)	129	WP_001278270.1	hypothetical protein	-
EXE31_RS13210	pilT	2708759..2709739 (-)	981	WP_001295381.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
EXE31_RS13215	yggS	2709757..2710461 (+)	705	WP_000997795.1	pyridoxal phosphate homeostasis protein	-
EXE31_RS13220	yggT	2710479..2711045 (+)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
EXE31_RS13225	yggU	2711042..2711332 (+)	291	WP_000994920.1	DUF167 family protein YggU	-
EXE31_RS13230	rdgB	2711340..2711933 (+)	594	WP_001174747.1	XTP/dITP diphosphatase	-
EXE31_RS13235	hemW	2711926..2713062 (+)	1137	WP_000239918.1	radical SAM family heme chaperone HemW	-
EXE31_RS13240	yggM	2713217..2714224 (-)	1008	WP_000745210.1	DUF1202 family protein	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35926.14 Da
• Isoelectric Point: 5.7654

>NTDB_id=305892 EXE31_RS13210 WP_001295381.1 2708759..2709739(-) (pilT) [Escherichia coli TW11681]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGMILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQHR
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=305892 EXE31_RS13210 WP_001295381.1 2708759..2709739(-) (pilT) [Escherichia coli TW11681]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCATGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGGTTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTACGTGACAGTGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACCTTACATACACGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGACCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCACCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (62-239)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Neisseria meningitidis 8013	49.085	100	0.494
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	48.93	100	0.491
pilT	Vibrio cholerae strain A1552	48.93	100	0.491
pilT	Neisseria gonorrhoeae MS11	48.78	100	0.491
pilT	Acinetobacter baumannii D1279779	46.483	100	0.466
pilT	Acinetobacter nosocomialis M2	46.483	100	0.466
pilT	Acinetobacter baumannii strain A118	46.483	100	0.466
pilT	Pseudomonas stutzeri DSM 10701	46.177	100	0.463
pilT	Acinetobacter baylyi ADP1	45.872	100	0.46
pilT	Pseudomonas aeruginosa PAK	45.566	100	0.457
pilT	Legionella pneumophila strain ERS1305867	45.912	97.546	0.448
pilT	Legionella pneumophila strain Lp02	45.912	97.546	0.448
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.824	97.546	0.408
pilU	Vibrio cholerae strain A1552	40.062	98.773	0.396
pilU	Pseudomonas stutzeri DSM 10701	37.273	100	0.377
pilU	Acinetobacter baylyi ADP1	37.048	100	0.377
pilB	Legionella pneumophila strain ERS1305867	30.89	100	0.362