Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   DV401_RS04475 Genome accession   NZ_CP031254
Coordinates   879910..880449 (-) Length   179 a.a.
NCBI ID   WP_071162831.1    Uniprot ID   -
Organism   Haemophilus influenzae strain M25588     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 874910..885449
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DV401_RS04445 fdx 875322..875663 (+) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -
  DV401_RS04450 iscX 875663..875857 (+) 195 WP_005659903.1 Fe-S cluster assembly protein IscX -
  DV401_RS04455 - 875905..876513 (-) 609 WP_114877309.1 YfgM family protein -
  DV401_RS04460 hisS 876531..877802 (-) 1272 WP_071162833.1 histidine--tRNA ligase -
  DV401_RS04465 ispG 877812..878918 (-) 1107 WP_005654847.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  DV401_RS04470 - 878927..879838 (-) 912 WP_071162832.1 RodZ domain-containing protein -
  DV401_RS04475 pilF/pilF2 879910..880449 (-) 540 WP_071162831.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  DV401_RS04480 - 880550..881701 (-) 1152 WP_042594886.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  DV401_RS04485 - 881948..882826 (+) 879 WP_005656360.1 D-alanyl-D-alanine carboxypeptidase family protein -
  DV401_RS04490 - 883191..884072 (+) 882 WP_071162830.1 metal ABC transporter substrate-binding protein -
  DV401_RS04495 - 884072..884992 (+) 921 WP_040034370.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20623.26 Da        Isoelectric Point: 8.2571

>NTDB_id=305101 DV401_RS04475 WP_071162831.1 879910..880449(-) (pilF/pilF2) [Haemophilus influenzae strain M25588]
MKTINTQLSAVIFPFIFSACVSQSASNLNHQAAAKVRVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGQIENAFREYEIAVNLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIVLCAYSAQKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=305101 DV401_RS04475 WP_071162831.1 879910..880449(-) (pilF/pilF2) [Haemophilus influenzae strain M25588]
ATGAAAACAATCAATACACAACTAAGTGCGGTTATTTTCCCTTTCATTTTTTCTGCTTGCGTTTCACAATCTGCATCTAA
CTTAAATCATCAAGCTGCCGCTAAAGTGCGTGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGCAAATAGAAAATGCCTTTCGTGAGTATGAAATAGCCGTAAACCTTAATCATAAACAAGGCGATGTGCATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAACAAGCCCAGCAACAATTTGAATTAGCACTTAATTCGCCGAATT
ATTATCATCAAGCAGATACATTTGAAAATATTGTGCTTTGTGCTTATTCCGCGCAAAAAATGGATATTTATCAGCAAACA
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGTCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

97.207

100

0.972
  pilF Glaesserella parasuis strain SC1401

44.633

98.883

0.441

Multiple sequence alignment