Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EXA09_RS01110 Genome accession   NZ_CP035812
Coordinates   249208..249648 (+) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain B7A     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 244208..254648
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXA09_RS01090 aroP 245206..246579 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  EXA09_RS01095 ampE 246622..247476 (-) 855 WP_000172000.1 beta-lactamase regulator AmpE -
  EXA09_RS01100 ampD 247473..248024 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EXA09_RS01105 nadC 248112..249005 (+) 894 WP_001297055.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EXA09_RS01110 pilA 249208..249648 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  EXA09_RS01115 pilB 249658..251043 (+) 1386 WP_001025174.1 type II secretion system protein GspE Machinery gene
  EXA09_RS01120 hofC 251033..252235 (+) 1203 WP_000157236.1 protein transport protein HofC -
  EXA09_RS01125 guaC 252270..253313 (-) 1044 WP_001217338.1 GMP reductase -
  EXA09_RS01130 - 253469..253513 (-) 45 WP_120795372.1 protein YacM -
  EXA09_RS01135 coaE 253538..254158 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=305030 EXA09_RS01110 WP_000360895.1 249208..249648(+) (pilA) [Escherichia coli strain B7A]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=305030 EXA09_RS01110 WP_000360895.1 249208..249648(+) (pilA) [Escherichia coli strain B7A]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment