Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   DV389_RS04895 Genome accession   NZ_CP031250
Coordinates   982611..983150 (-) Length   179 a.a.
NCBI ID   WP_005649140.1    Uniprot ID   -
Organism   Haemophilus influenzae strain M21384     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 977611..988150
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DV389_RS04865 (DV389_04865) fdx 978014..978355 (+) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -
  DV389_RS04870 (DV389_04870) iscX 978355..978549 (+) 195 WP_005659903.1 Fe-S cluster assembly protein IscX -
  DV389_RS04875 (DV389_04875) - 978600..979214 (-) 615 WP_050847273.1 YfgM family protein -
  DV389_RS04880 (DV389_04880) hisS 979232..980503 (-) 1272 WP_114893257.1 histidine--tRNA ligase -
  DV389_RS04885 (DV389_04885) ispG 980513..981619 (-) 1107 WP_048954971.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  DV389_RS04890 (DV389_04890) - 981628..982539 (-) 912 WP_114893258.1 RodZ family helix-turn-helix domain-containing protein -
  DV389_RS04895 (DV389_04895) pilF/pilF2 982611..983150 (-) 540 WP_005649140.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  DV389_RS04900 (DV389_04900) - 983251..984402 (-) 1152 WP_162697479.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  DV389_RS04905 (DV389_04905) - 984649..985527 (+) 879 WP_005649130.1 serine hydrolase -
  DV389_RS04910 (DV389_04910) - 985889..986770 (+) 882 WP_005669100.1 metal ABC transporter substrate-binding protein -
  DV389_RS04915 (DV389_04915) - 986770..987690 (+) 921 WP_050822774.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20555.22 Da        Isoelectric Point: 8.6194

>NTDB_id=304959 DV389_RS04895 WP_005649140.1 982611..983150(-) (pilF/pilF2) [Haemophilus influenzae strain M21384]
MQTISKQLSAVIFPFIFSACVSQSASSLDHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGQIENAFREYEIAVKLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIALCAYSAKKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=304959 DV389_RS04895 WP_005649140.1 982611..983150(-) (pilF/pilF2) [Haemophilus influenzae strain M21384]
ATGCAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTCATTTTTTCTGCTTGCGTTTCACAATCTGCGTCTAG
CTTAGATCATCAAGCTGCCGCCAAAGCGCGAGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTACATTCAGCACTTGCACATTATTATCAACAA
CAAGGGCAAATAGAAAATGCCTTTCGTGAGTATGAAATAGCCGTAAAACTTAATCATAAACAAGGCGATGTACATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAGCAAGCCCAGCAACAATTTGAATTAGCCCTTAATTCGCCGAATT
ATTATCATCAAGCAGATACATTTGAAAATATCGCGCTTTGTGCTTATTCCGCGAAAAAAATGGATATTTATCAGCAAACA
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGCCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

96.648

100

0.966
  pilF Glaesserella parasuis strain SC1401

45.81

100

0.458

Multiple sequence alignment