Detailed information    

insolico Bioinformatically predicted

Overview

Name   uvrC   Type   Machinery gene
Locus tag   EW648_RS05685 Genome accession   NZ_CP035772
Coordinates   1198421..1200253 (+) Length   610 a.a.
NCBI ID   WP_001283421.1    Uniprot ID   P0A8G1
Organism   Escherichia coli O145 strain RM8843-C1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1193421..1205253
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW648_RS05650 (EW648_05760) dcyD 1193647..1194633 (+) 987 WP_001128215.1 D-cysteine desulfhydrase -
  EW648_RS05655 (EW648_05765) tcyL 1194648..1195316 (+) 669 WP_001158220.1 cystine ABC transporter permease -
  EW648_RS05660 (EW648_05770) tcyN 1195313..1196065 (+) 753 WP_001272991.1 L-cystine ABC transporter ATP-binding protein TcyN -
  EW648_RS05665 (EW648_05775) sdiA 1196295..1197017 (+) 723 WP_001154283.1 transcriptional regulator SdiA -
  EW648_RS05670 (EW648_05780) yecF 1197085..1197309 (-) 225 WP_000106476.1 DUF2594 family protein YecF -
  EW648_RS05675 (EW648_05785) yecU 1197296..1197472 (-) 177 WP_001307856.1 protein YecU -
  EW648_RS05680 (EW648_05790) letA 1197768..1198424 (+) 657 WP_000611328.1 UvrY/SirA/GacA family response regulator transcription factor Regulator
  EW648_RS05685 (EW648_05795) uvrC 1198421..1200253 (+) 1833 WP_001283421.1 excinuclease ABC subunit UvrC Machinery gene
  EW648_RS05690 (EW648_05800) pgsA 1200310..1200858 (+) 549 WP_001160187.1 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase -
  EW648_RS27415 - 1201852..1202022 (+) 171 Protein_1122 hypothetical protein -
  EW648_RS27420 - 1203318..1203446 (+) 129 Protein_1124 hypothetical protein -
  EW648_RS05720 (EW648_05835) - 1203636..1203776 (-) 141 WP_000078920.1 Hok/Gef family protein -
  EW648_RS05725 (EW648_05840) - 1203966..1204226 (-) 261 WP_000488107.1 ogr/Delta-like zinc finger family protein -
  EW648_RS05730 (EW648_05845) - 1204269..1205069 (-) 801 WP_244448995.1 contractile injection system protein, VgrG/Pvc8 family -
  EW648_RS27850 - 1205030..1205113 (+) 84 Protein_1128 hypothetical protein -

Sequence

Protein


Download         Length: 610 a.a.        Molecular weight: 68188.03 Da        Isoelectric Point: 9.3685

>NTDB_id=304880 EW648_RS05685 WP_001283421.1 1198421..1200253(+) (uvrC) [Escherichia coli O145 strain RM8843-C1]
MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLSSYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLL
EHNYIKLYQPRYNVLLRDDKSYPFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETASQNLEFEEAARIRDQIQAVRRVT
EKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKVLGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLD
FNLSDKTLLADSLSELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEVKRMECFDI
SHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRYGKAIDDSKIPDVILIDGGKGQLAQAKNVFA
ELDVSWDKNHPLLLGVAKGADRKAGLETLFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLET
IEGVGPKRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH

Nucleotide


Download         Length: 1833 bp        

>NTDB_id=304880 EW648_RS05685 WP_001283421.1 1198421..1200253(+) (uvrC) [Escherichia coli O145 strain RM8843-C1]
GTGAGTGATCAGTTTGACGCAAAAGCGTTTTTAAAAACCGTAACCAGCCAGCCAGGCGTTTATCGCATGTACGATGCTGG
TGGTACGGTTATCTATGTCGGCAAAGCGAAAGACCTGAAAAAACGGCTTTCCAGCTATTTCCGTAGCAACCTCGCTTCGC
GCAAAACCGAAGCGCTGGTCGCCCAGATCCAGCAAATTGATGTAACGGTTACTCATACAGAAACCGAAGCGCTGTTGCTG
GAACACAACTACATCAAACTCTATCAGCCGCGTTACAACGTTTTGCTACGCGATGATAAATCTTATCCCTTTATCTTCCT
GAGTGGCGATACCCACCCGCGTCTGGCGATGCATCGTGGTGCGAAGCATGCCAAAGGTGAATATTTTGGCCCGTTCCCGA
ATGGCTATGCCGTACGTGAAACACTGGCGCTACTGCAAAAGATATTCCCCATTCGCCAGTGCGAAAATAGTGTTTATCGC
AATCGCTCGCGTCCGTGTCTGCAATATCAGATAGGGCGCTGTCTGGGACCGTGCGTTGAAGGACTGGTGAGTGAAGAAGA
ATACGCTCAGCAGGTCGAGTATGTGCGCCTGTTTTTGTCTGGCAAAGATGATCAGGTGCTTACGCAACTCATTAGCCGTA
TGGAAACTGCCAGCCAGAATCTGGAGTTTGAAGAAGCGGCACGTATTCGCGACCAAATTCAGGCGGTGCGACGCGTCACC
GAAAAACAGTTTGTGTCCAATACCGGCGACGACCTTGACGTTATTGGTGTGGCGTTCGATGCGGGCATGGCTTGTGTCCA
CGTATTGTTCATTCGTCAGGGCAAAGTGCTCGGCAGCCGCAGCTATTTCCCGAAAGTGCCTGGCGGTACGGAACTGAGCG
AGGTGGTGGAAACCTTCGTAGGACAGTTCTATTTACAAGGCAGCCAGATGCGCACCTTACCGGGTGAGATCCTGCTCGAT
TTTAATCTTAGCGATAAAACGCTGCTCGCCGATTCCCTTTCAGAACTGGCGGGACGCAAGATTAATGTTCAAACCAAACC
TCGCGGCGATAGGGCGCGTTATCTGAAACTCGCGCGCACCAATGCGGCGACGGCCTTAACCAGCAAACTTTCGCAGCAAT
CTACCGTTCACCAGCGACTGACCGCGCTTGCCAGCGTGTTGAAATTGCCGGAAGTGAAGCGGATGGAGTGCTTTGACATC
AGCCATACCATGGGTGAACAAACCGTCGCTTCCTGTGTGGTGTTTGATGCTAACGGCCCGCTGCGTGCGGAGTATCGGCG
CTATAACATTACTGGCATCACGCCGGGCGATGATTATGCGGCGATGAATCAGGTGCTGCGTCGGCGTTATGGTAAAGCCA
TCGACGACAGTAAGATCCCGGATGTGATACTTATCGACGGCGGCAAAGGCCAGCTTGCGCAGGCGAAAAATGTCTTCGCC
GAACTGGATGTCTCATGGGATAAAAATCATCCGCTGCTACTTGGCGTTGCCAAAGGAGCAGATCGTAAGGCTGGGCTGGA
AACGCTGTTCTTTGAGCCGGAAGGTGAGGGATTCAGTTTGCCGCCAGATTCTCCCGCGCTGCATGTTATCCAGCATATTC
GCGATGAATCACATGATCACGCGATTGGCGGGCACCGTAAAAAACGGGCGAAGGTCAAAAATACCAGTTCCCTGGAAACC
ATTGAAGGCGTCGGGCCAAAACGCCGGCAAATGTTGTTGAAATATATGGGTGGTTTGCAAGGTTTACGTAACGCCAGCGT
CGAGGAAATTGCAAAAGTGCCGGGTATTTCGCAAGGTCTGGCAGAAAAGATCTTCTGGTCGTTGAAACATTGA

Domains

Predicted by InterProScan.

UvrB/uvrC motif (UVR)

(205-237)

Helix-hairpin-helix domain (HHH_5)

(558-609)

UvrC RNAse H endonuclease domain (UvrC_RNaseH_dom)

(385-542)

GIY-YIG catalytic domain (GIY-YIG)

(18-93)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P0A8G1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  uvrC Streptococcus pneumoniae TIGR4

37.891

99.508

0.377
  uvrC Streptococcus pneumoniae R6

37.562

99.508

0.374
  uvrC Streptococcus pneumoniae D39

37.562

99.508

0.374