Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   DV428_RS08395 Genome accession   NZ_CP031243
Coordinates   1731354..1731896 (+) Length   180 a.a.
NCBI ID   WP_114909389.1    Uniprot ID   -
Organism   Haemophilus haemolyticus strain M19346     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1726354..1736896
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DV428_RS08375 - 1727189..1728049 (-) 861 WP_239989706.1 metal ABC transporter substrate-binding protein -
  DV428_RS08380 - 1728427..1728894 (+) 468 WP_114909386.1 hypothetical protein -
  DV428_RS08385 - 1728978..1729856 (-) 879 WP_114909387.1 serine hydrolase -
  DV428_RS08390 - 1730102..1731253 (+) 1152 WP_162790804.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  DV428_RS08395 pilF/pilF2 1731354..1731896 (+) 543 WP_114909389.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  DV428_RS08400 - 1731967..1732878 (+) 912 WP_114909390.1 RodZ family helix-turn-helix domain-containing protein -
  DV428_RS08405 ispG 1732890..1733996 (+) 1107 WP_114909391.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  DV428_RS08410 hisS 1734006..1735277 (+) 1272 WP_114909392.1 histidine--tRNA ligase -
  DV428_RS08415 - 1735295..1735909 (+) 615 WP_114909393.1 YfgM family protein -
  DV428_RS08420 iscX 1735960..1736154 (-) 195 WP_114909394.1 Fe-S cluster assembly protein IscX -
  DV428_RS08425 fdx 1736154..1736495 (-) 342 WP_005629440.1 ISC system 2Fe-2S type ferredoxin -

Sequence

Protein


Download         Length: 180 a.a.        Molecular weight: 20881.38 Da        Isoelectric Point: 7.3959

>NTDB_id=304585 DV428_RS08395 WP_114909389.1 1731354..1731896(+) (pilF/pilF2) [Haemophilus haemolyticus strain M19346]
MQTISKQLSAVIFPFIFSACVSQSTIQDFNQQSAAKARVELALGYLQQNNPQLAKINLDKALQHDNDYYLVHSALAHYYQ
QQGEIENAHREYEIALKLNNQQGDLHNNFGTFLCRQKQFEQAQQQFQLALNSPNYYHQADTFENMALCAYSAQKMDIYQQ
ALQKLRQTDSKRAEKFNTFK

Nucleotide


Download         Length: 543 bp        

>NTDB_id=304585 DV428_RS08395 WP_114909389.1 1731354..1731896(+) (pilF/pilF2) [Haemophilus haemolyticus strain M19346]
ATGCAAACAATAAGTAAACAACTCAGTGCGGTTATTTTCCCTTTCATTTTTTCTGCTTGCGTTTCCCAATCAACTATTCA
AGATTTCAATCAACAATCAGCAGCGAAAGCGCGCGTAGAACTCGCACTCGGTTACCTTCAACAAAATAATCCTCAACTCG
CTAAAATCAACTTAGATAAAGCACTTCAACACGATAACGATTACTATCTCGTGCATTCAGCACTTGCGCATTATTATCAA
CAACAAGGTGAAATCGAGAACGCACATCGTGAATATGAAATAGCTTTAAAACTCAATAATCAACAGGGAGACTTGCATAA
TAATTTTGGTACATTTTTATGTCGTCAAAAACAATTTGAACAAGCTCAGCAGCAATTTCAACTCGCACTAAATTCCCCTA
ATTATTATCATCAAGCAGATACATTTGAAAATATGGCTCTTTGTGCTTATTCGGCTCAAAAAATGGATATTTATCAACAA
GCATTACAAAAATTGCGACAAACTGATAGTAAACGAGCGGAAAAATTTAATACCTTCAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

83.889

100

0.839
  pilF Glaesserella parasuis strain SC1401

47.253

100

0.478

Multiple sequence alignment