Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   DV384_RS05655 Genome accession   NZ_CP031241
Coordinates   1160205..1160744 (+) Length   179 a.a.
NCBI ID   WP_114934575.1    Uniprot ID   -
Organism   Haemophilus influenzae strain M17648     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1155205..1165744
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DV384_RS05635 - 1155662..1156582 (-) 921 WP_005654835.1 ATP-binding cassette domain-containing protein -
  DV384_RS05640 - 1156582..1157463 (-) 882 WP_005662662.1 metal ABC transporter substrate-binding protein -
  DV384_RS05645 - 1157828..1158706 (-) 879 WP_005659920.1 D-alanyl-D-alanine carboxypeptidase family protein -
  DV384_RS05650 - 1158953..1160104 (+) 1152 WP_065251304.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  DV384_RS05655 pilF/pilF2 1160205..1160744 (+) 540 WP_114934575.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  DV384_RS05660 - 1160816..1161727 (+) 912 WP_005659913.1 RodZ family helix-turn-helix domain-containing protein -
  DV384_RS05665 ispG 1161736..1162842 (+) 1107 WP_114934576.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  DV384_RS05670 hisS 1162852..1164123 (+) 1272 WP_114934577.1 histidine--tRNA ligase -
  DV384_RS05675 - 1164141..1164755 (+) 615 WP_112109782.1 YfgM family protein -
  DV384_RS05680 iscX 1164806..1165000 (-) 195 WP_005659903.1 Fe-S cluster assembly protein IscX -
  DV384_RS05685 fdx 1165000..1165341 (-) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20629.26 Da        Isoelectric Point: 8.6216

>NTDB_id=304498 DV384_RS05655 WP_114934575.1 1160205..1160744(+) (pilF/pilF2) [Haemophilus influenzae strain M17648]
MKTISKQLSAVIFPFIFSACVSQSASNLNHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGQIENAFREYEIAVNLNHKQGDVHNNFGTFLCSQKKFEQAQQQFEFALNSPNYYHQADTFENIVLCAYSAQKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=304498 DV384_RS05655 WP_114934575.1 1160205..1160744(+) (pilF/pilF2) [Haemophilus influenzae strain M17648]
ATGAAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTCATTTTTTCTGCCTGCGTTTCACAATCTGCATCTAA
CTTAAATCATCAAGCTGCCGCTAAAGCGCGTGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTAGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGCAAATAGAAAATGCCTTTCGTGAGTATGAAATAGCCGTAAACCTTAATCATAAACAAGGCGATGTGCATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAACAAGCCCAGCAACAATTTGAATTCGCACTTAATTCGCCGAATT
ATTATCATCAAGCAGATACATTTGAAAATATTGTGCTTTGTGCTTATTCCGCGCAAAAAATGGATATTTATCAGCAAACT
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGTCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

98.324

100

0.983
  pilF Glaesserella parasuis strain SC1401

44.693

100

0.447

Multiple sequence alignment