Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   DV441_RS01880 Genome accession   NZ_CP031238
Coordinates   422731..423273 (+) Length   180 a.a.
NCBI ID   WP_046953121.1    Uniprot ID   A0A0M3G929
Organism   Haemophilus haemolyticus strain M28486     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 417731..428273
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DV441_RS01860 - 418350..419270 (-) 921 WP_032825517.1 ATP-binding cassette domain-containing protein -
  DV441_RS01865 - 419270..420151 (-) 882 WP_046953124.1 metal ABC transporter substrate-binding protein -
  DV441_RS01870 - 420354..421232 (-) 879 WP_046953123.1 D-alanyl-D-alanine carboxypeptidase family protein -
  DV441_RS01875 - 421479..422630 (+) 1152 WP_046953122.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  DV441_RS01880 pilF/pilF2 422731..423273 (+) 543 WP_046953121.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  DV441_RS01885 - 423344..424255 (+) 912 WP_046953120.1 RodZ domain-containing protein -
  DV441_RS01890 ispG 424267..425373 (+) 1107 WP_005630108.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  DV441_RS01895 hisS 425383..426654 (+) 1272 WP_046953119.1 histidine--tRNA ligase -
  DV441_RS01900 - 426672..427286 (+) 615 WP_046953118.1 YfgM family protein -
  DV441_RS01905 iscX 427337..427531 (-) 195 WP_005626949.1 Fe-S cluster assembly protein IscX -
  DV441_RS01910 fdx 427531..427872 (-) 342 WP_005640724.1 ISC system 2Fe-2S type ferredoxin -

Sequence

Protein


Download         Length: 180 a.a.        Molecular weight: 20884.47 Da        Isoelectric Point: 7.8275

>NTDB_id=304382 DV441_RS01880 WP_046953121.1 422731..423273(+) (pilF/pilF2) [Haemophilus haemolyticus strain M28486]
MQTISKQLSAVIFPFIFSACVFQSTIQDFNQQSAAKARVELALGYLQQGNPQLAKINLDKALQHDKDYYLVHSGLAHYYQ
QQGEIENAHREYEIALKLNNQQGDLHNNFGTFLCRQKQFEQAQQQFQLALNSPNYYHQADTFENMAICAYSAQKMDIYQQ
ALQKLRQTDSKRAEKFNTFK

Nucleotide


Download         Length: 543 bp        

>NTDB_id=304382 DV441_RS01880 WP_046953121.1 422731..423273(+) (pilF/pilF2) [Haemophilus haemolyticus strain M28486]
ATGCAAACAATAAGTAAACAATTAAGTGCGGTTATTTTCCCTTTCATTTTTTCTGCTTGCGTTTTTCAATCAACAATTCA
AGATTTTAATCAACAATCAGCAGCGAAAGCTCGTGTAGAACTTGCCCTCGGTTACCTTCAACAAGGTAATCCTCAACTAG
CCAAAATCAACTTAGATAAAGCACTTCAACACGATAAAGATTACTATCTCGTGCATTCAGGGCTTGCGCATTATTATCAG
CAACAAGGGGAAATCGAGAATGCACATCGTGAATATGAAATAGCTTTAAAGCTCAATAATCAACAGGGAGACTTGCATAA
TAATTTTGGTACATTTTTATGTCGTCAAAAGCAATTTGAACAAGCTCAACAGCAATTTCAACTCGCACTGAATTCACCTA
ATTATTATCATCAAGCAGATACATTTGAAAATATGGCTATTTGTGCTTATTCCGCTCAAAAAATGGATATTTATCAACAA
GCATTACAAAAATTGCGACAAACTGATAGTAAACGAGCGGAAAAATTTAATACCTTCAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0M3G929

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

82.222

100

0.822
  pilF Glaesserella parasuis strain SC1401

46.703

100

0.472

Multiple sequence alignment