Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   DV365_RS00945 Genome accession   NZ_CP031237
Coordinates   182165..182704 (-) Length   179 a.a.
NCBI ID   WP_005649140.1    Uniprot ID   -
Organism   Haemophilus influenzae strain M12125     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 177165..187704
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DV365_RS00915 fdx 177568..177909 (+) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -
  DV365_RS00920 iscX 177909..178103 (+) 195 WP_005659903.1 Fe-S cluster assembly protein IscX -
  DV365_RS00925 - 178154..178768 (-) 615 WP_112109782.1 YfgM family protein -
  DV365_RS00930 hisS 178786..180057 (-) 1272 WP_114890098.1 histidine--tRNA ligase -
  DV365_RS00935 ispG 180067..181173 (-) 1107 WP_021035647.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  DV365_RS00940 - 181182..182093 (-) 912 WP_042599460.1 RodZ family helix-turn-helix domain-containing protein -
  DV365_RS00945 pilF/pilF2 182165..182704 (-) 540 WP_005649140.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  DV365_RS00950 - 182804..183955 (-) 1152 WP_042599461.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  DV365_RS00955 - 184202..185080 (+) 879 WP_042599462.1 D-alanyl-D-alanine carboxypeptidase family protein -
  DV365_RS00960 - 185471..186331 (+) 861 WP_223364826.1 metal ABC transporter substrate-binding protein -
  DV365_RS00965 - 186331..187251 (+) 921 WP_114890099.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20555.22 Da        Isoelectric Point: 8.6194

>NTDB_id=304354 DV365_RS00945 WP_005649140.1 182165..182704(-) (pilF/pilF2) [Haemophilus influenzae strain M12125]
MQTISKQLSAVIFPFIFSACVSQSASSLDHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGQIENAFREYEIAVKLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIALCAYSAKKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=304354 DV365_RS00945 WP_005649140.1 182165..182704(-) (pilF/pilF2) [Haemophilus influenzae strain M12125]
ATGCAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTCATTTTTTCTGCCTGCGTTTCACAATCTGCGTCTAG
CTTAGATCATCAAGCTGCCGCCAAAGCGCGAGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTACATTCAGCACTTGCACATTATTATCAACAA
CAAGGGCAAATAGAAAATGCCTTTCGTGAGTATGAAATAGCCGTAAAACTTAATCATAAACAAGGCGATGTACATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAGCAAGCCCAGCAACAATTTGAATTAGCCCTTAATTCGCCGAATT
ATTATCATCAAGCAGATACATTTGAAAATATCGCGCTTTGTGCTTATTCCGCGAAAAAAATGGATATTTATCAGCAAACA
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGCCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

96.648

100

0.966
  pilF Glaesserella parasuis strain SC1401

45.81

100

0.458

Multiple sequence alignment