Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   DV400_RS01780 Genome accession   NZ_CP031236
Coordinates   341888..342427 (-) Length   179 a.a.
NCBI ID   WP_005649140.1    Uniprot ID   -
Organism   Haemophilus influenzae strain M25267     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 336888..347427
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DV400_RS01750 fdx 337291..337632 (+) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -
  DV400_RS01755 iscX 337632..337826 (+) 195 WP_005659903.1 Fe-S cluster assembly protein IscX -
  DV400_RS01760 - 337877..338491 (-) 615 WP_005691752.1 YfgM family protein -
  DV400_RS01765 hisS 338509..339780 (-) 1272 WP_021035648.1 histidine--tRNA ligase -
  DV400_RS01770 ispG 339790..340896 (-) 1107 WP_021035647.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  DV400_RS01775 - 340905..341816 (-) 912 WP_042599460.1 RodZ family helix-turn-helix domain-containing protein -
  DV400_RS01780 pilF/pilF2 341888..342427 (-) 540 WP_005649140.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  DV400_RS01785 - 342527..343678 (-) 1152 WP_042599461.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  DV400_RS01790 - 343925..344803 (+) 879 WP_005669102.1 serine hydrolase -
  DV400_RS01795 - 345165..346046 (+) 882 WP_005669100.1 metal ABC transporter substrate-binding protein -
  DV400_RS01800 - 346046..346966 (+) 921 WP_005669098.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20555.22 Da        Isoelectric Point: 8.6194

>NTDB_id=304327 DV400_RS01780 WP_005649140.1 341888..342427(-) (pilF/pilF2) [Haemophilus influenzae strain M25267]
MQTISKQLSAVIFPFIFSACVSQSASSLDHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGQIENAFREYEIAVKLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIALCAYSAKKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=304327 DV400_RS01780 WP_005649140.1 341888..342427(-) (pilF/pilF2) [Haemophilus influenzae strain M25267]
ATGCAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTCATTTTTTCTGCCTGCGTTTCACAATCTGCGTCTAG
CTTAGATCATCAAGCTGCCGCCAAAGCGCGAGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTACATTCAGCACTTGCACATTATTATCAACAA
CAAGGGCAAATAGAAAATGCCTTTCGTGAGTATGAAATAGCCGTAAAACTTAATCATAAACAAGGCGATGTACATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAGCAAGCCCAGCAACAATTTGAATTAGCCCTTAATTCGCCGAATT
ATTATCATCAAGCAGATACATTTGAAAATATCGCGCTTTGTGCTTATTCCGCGAAAAAAATGGATATTTATCAGCAAACA
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGCCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

96.648

100

0.966
  pilF Glaesserella parasuis strain SC1401

45.81

100

0.458

Multiple sequence alignment