Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DV702_RS12335 Genome accession   NZ_CP031230
Coordinates   2561726..2563096 (-) Length   456 a.a.
NCBI ID   WP_114925015.1    Uniprot ID   -
Organism   Sporosarcina sp. PTS2304     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2556726..2568096
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DV702_RS12315 (DV702_12315) gltX 2557765..2559225 (-) 1461 WP_114925013.1 glutamate--tRNA ligase -
  DV702_RS12320 (DV702_12320) ispF 2559308..2559787 (-) 480 WP_114925930.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  DV702_RS12325 (DV702_12325) ispD 2559796..2560467 (-) 672 WP_114925931.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  DV702_RS12330 (DV702_12330) - 2560496..2561587 (-) 1092 WP_114925014.1 PIN/TRAM domain-containing protein -
  DV702_RS12335 (DV702_12335) radA 2561726..2563096 (-) 1371 WP_114925015.1 DNA repair protein RadA Machinery gene
  DV702_RS12340 (DV702_12340) - 2563186..2565633 (-) 2448 WP_114925932.1 ATP-dependent Clp protease ATP-binding subunit -
  DV702_RS12345 (DV702_12345) - 2565821..2566906 (-) 1086 WP_114925016.1 protein arginine kinase -
  DV702_RS12350 (DV702_12350) - 2566896..2567459 (-) 564 WP_114925017.1 UvrB/UvrC motif-containing protein -
  DV702_RS12355 (DV702_12355) - 2567456..2567926 (-) 471 WP_114925018.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 50081.82 Da        Isoelectric Point: 6.9006

>NTDB_id=304276 DV702_RS12335 WP_114925015.1 2561726..2563096(-) (radA) [Sporosarcina sp. PTS2304]
MAKRKSKFMCRSCGYESAKWMGRCPGCGEWNTMDEEVEIVQKGPRGAFQHGDKVKQKALPISKVETVEEPRVKTELEELN
RVLGGGIVPGSLILIGGDPGIGKSTLLLQVSSLLANQQQRVLYISGEESIRQTKLRAERLGVKSDELYIYAETDLSMIHE
TVDEVQPKFVIVDSIQTVHHPEVTSAPGSVSQVRECTAELMRIAKTQNIAIFIVGHVTKEGQIAGPRLLEHMVDTVLYFE
GERHHTYRILRSVKNRFGSTNEIAIFEMLQSGLKEVLNPSELFLRERSQGGAGSTIVASMEGTRPILVEIQALMTASSFN
YPKRMATGLEQNRVSLLMAVLEKRAGMLLQTQDAYIKVAGGVKLDEPAIDLAVLLSIVSSYKDTAVGARDCFVGEVGLTG
EVRRVSRIEQRVTEAAKLGFDRVIIPASNMGGWDIPSGIEVVGVETIKEALGIAFK

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=304276 DV702_RS12335 WP_114925015.1 2561726..2563096(-) (radA) [Sporosarcina sp. PTS2304]
ATGGCAAAGCGTAAATCGAAATTTATGTGTCGTTCATGCGGATATGAGTCCGCTAAATGGATGGGACGCTGTCCGGGTTG
CGGAGAATGGAATACGATGGATGAAGAAGTAGAGATTGTTCAAAAAGGACCGCGTGGCGCTTTTCAGCATGGTGATAAGG
TAAAACAGAAAGCATTGCCTATTAGTAAAGTGGAAACGGTGGAAGAACCGCGTGTGAAAACAGAACTGGAAGAACTAAAC
CGTGTGTTAGGCGGGGGAATTGTTCCGGGGTCTCTAATTTTAATCGGAGGAGATCCGGGGATCGGTAAATCTACGTTGTT
GCTCCAAGTATCTTCTTTGTTAGCGAACCAACAGCAGCGTGTGCTTTACATTTCAGGTGAAGAGTCTATTCGTCAGACGA
AACTGCGAGCAGAAAGATTAGGCGTAAAATCAGACGAACTTTATATTTACGCAGAGACAGATCTTAGCATGATTCATGAA
ACAGTGGATGAAGTGCAGCCGAAGTTTGTAATTGTCGACTCCATCCAGACGGTACATCATCCTGAAGTTACCTCAGCACC
GGGGAGCGTCTCCCAAGTACGTGAGTGTACAGCAGAACTGATGAGAATAGCAAAAACACAAAACATCGCTATATTCATCG
TCGGTCATGTAACCAAGGAAGGTCAAATTGCCGGGCCACGGCTGCTTGAACATATGGTAGATACGGTATTGTATTTTGAA
GGGGAACGACACCATACCTATCGGATTCTACGCAGTGTGAAAAACCGTTTTGGTTCAACAAATGAGATCGCGATATTCGA
GATGTTGCAGTCTGGATTAAAAGAAGTGTTGAACCCCTCGGAATTATTTTTACGTGAACGTTCACAAGGCGGAGCAGGTT
CCACTATTGTAGCGTCTATGGAAGGTACGCGGCCTATTCTAGTAGAAATACAGGCGTTAATGACGGCTTCTAGTTTTAAT
TATCCGAAGCGAATGGCAACGGGATTGGAGCAAAATCGTGTGTCGCTCCTAATGGCTGTACTGGAAAAACGTGCAGGAAT
GCTATTGCAGACACAAGATGCGTATATTAAAGTGGCCGGTGGCGTGAAATTGGATGAGCCGGCAATCGACTTGGCTGTAT
TGCTTAGCATCGTCTCCAGTTATAAAGATACAGCTGTAGGAGCAAGGGATTGTTTTGTTGGAGAAGTGGGGTTAACAGGT
GAGGTAAGAAGGGTATCTAGAATAGAACAACGTGTGACAGAAGCAGCTAAGCTTGGATTTGATCGAGTCATCATCCCGGC
ATCGAACATGGGCGGATGGGATATTCCGTCCGGCATTGAAGTTGTTGGTGTAGAAACTATTAAAGAAGCGCTGGGTATCG
CATTTAAATGA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)

AAA domain (AAA_25)

(63-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

71.145

99.561

0.708
  radA Streptococcus pneumoniae Rx1

62.637

99.781

0.625
  radA Streptococcus pneumoniae D39

62.637

99.781

0.625
  radA Streptococcus pneumoniae R6

62.637

99.781

0.625
  radA Streptococcus pneumoniae TIGR4

62.637

99.781

0.625
  radA Streptococcus mitis NCTC 12261

62.637

99.781

0.625
  radA Streptococcus mitis SK321

62.637

99.781

0.625

Multiple sequence alignment