Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   U14A_RS20110 Genome accession   NZ_CP035516
Coordinates   4040252..4040920 (-) Length   222 a.a.
NCBI ID   WP_001296533.1    Uniprot ID   B7N279
Organism   Escherichia coli strain U14A     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4035252..4045920
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U14A_RS20080 (U14A_03869) rfaQ 4035429..4036487 (-) 1059 WP_000360408.1 lipopolysaccharide core heptosyltransferase RfaQ -
  U14A_RS20085 (U14A_03870) waaA 4036901..4038178 (+) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  U14A_RS20090 (U14A_03871) coaD 4038186..4038665 (+) 480 WP_001171854.1 pantetheine-phosphate adenylyltransferase -
  U14A_RS20095 (U14A_03872) mutM 4038704..4039513 (-) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  U14A_RS20100 (U14A_03873) rpmG 4039611..4039778 (-) 168 WP_001051798.1 50S ribosomal protein L33 -
  U14A_RS20105 (U14A_03874) rpmB 4039799..4040035 (-) 237 WP_000091955.1 50S ribosomal protein L28 -
  U14A_RS20110 (U14A_03875) radC 4040252..4040920 (-) 669 WP_001296533.1 DNA repair protein RadC Machinery gene
  U14A_RS20115 (U14A_03876) coaBC 4041092..4042312 (+) 1221 WP_000050112.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  U14A_RS20120 (U14A_03877) dut 4042290..4042748 (+) 459 WP_000976070.1 dUTP diphosphatase -
  U14A_RS20125 (U14A_03878) slmA 4042855..4043451 (+) 597 WP_000818603.1 nucleoid occlusion factor SlmA -
  U14A_RS20130 (U14A_03879) pyrE 4043488..4044129 (-) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  U14A_RS20135 (U14A_03880) rph 4044195..4044911 (-) 717 WP_001247093.1 ribonuclease PH -
  U14A_RS20140 (U14A_03881) yicC 4045038..4045901 (+) 864 WP_000621332.1 YicC/YloC family endoribonuclease -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25286.40 Da        Isoelectric Point: 7.9748

>NTDB_id=304012 U14A_RS20110 WP_001296533.1 4040252..4040920(-) (radC) [Escherichia coli strain U14A]
MKNNVQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=304012 U14A_RS20110 WP_001296533.1 4040252..4040920(-) (radC) [Escherichia coli strain U14A]
GTGAAAAACAATGTACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAGCAATTTAGTGGCGTTCATGGAATTGGCGTGGCGAAATTCGCCCAGTTA
AAGGGGATTGCCGAACTGGCGCGGCGTTACTACAACGTACGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GAGGGAATTTTTACAAAGCCAGCTCACGGGCGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACAGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCGCATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

UPF0758 N-terminal (UPF0758_N)

(10-77)

RadC-like JAB domain (RadC)

(103-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7N279

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment