Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   HSM_RS01865 Genome accession   NC_010519
Coordinates   406091..406630 (+) Length   179 a.a.
NCBI ID   WP_012341221.1    Uniprot ID   -
Organism   Histophilus somni 2336     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 401091..411630
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HSM_RS01845 (HSM_0361) lpxM 401092..402045 (+) 954 WP_012341217.1 lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase -
  HSM_RS01850 (HSM_0362) apt 402116..402655 (+) 540 WP_012341218.1 adenine phosphoribosyltransferase -
  HSM_RS01855 (HSM_0363) dnaX 402686..404689 (+) 2004 WP_012341219.1 DNA polymerase III subunit gamma/tau -
  HSM_RS01860 (HSM_0364) - 404942..406060 (+) 1119 WP_012341220.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  HSM_RS01865 (HSM_0365) pilF/pilF2 406091..406630 (+) 540 WP_012341221.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  HSM_RS01870 (HSM_0366) nrdA 406713..408983 (-) 2271 WP_012341222.1 class 1a ribonucleoside-diphosphate reductase subunit alpha -
  HSM_RS01875 (HSM_0367) dnaJ 409267..410388 (-) 1122 WP_012341223.1 molecular chaperone DnaJ -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20670.59 Da        Isoelectric Point: 9.3139

>NTDB_id=30398 HSM_RS01865 WP_012341221.1 406091..406630(+) (pilF/pilF2) [Histophilus somni 2336]
MRYRTIFTFIIFSLFLSACVSQPTTGRFNKQQAAKARIELGLGYLKLRNFSQAKLNFDKALFYQPDYYLVHSAFAYLYQL
QGDISQAKSSYEKAISLDKSQGDVKNNYGAFLCSQGEFARAYELFEQALTTPNYYQQADTYANIIICAEAEHNIPRYQKF
LQLLKNIDPKRAEEIKKVS

Nucleotide


Download         Length: 540 bp        

>NTDB_id=30398 HSM_RS01865 WP_012341221.1 406091..406630(+) (pilF/pilF2) [Histophilus somni 2336]
ATGCGATATCGGACTATATTTACTTTTATTATATTCTCTCTTTTTCTTTCTGCTTGCGTTTCTCAGCCTACAACTGGTCG
CTTTAATAAGCAACAAGCTGCAAAAGCACGAATTGAGTTAGGTTTAGGTTATTTAAAATTACGCAATTTTTCTCAAGCAA
AACTTAATTTTGATAAAGCTTTATTCTATCAACCTGACTATTATTTGGTACATTCGGCTTTTGCCTATTTATATCAATTA
CAAGGTGATATTTCGCAAGCGAAATCCTCTTATGAAAAAGCCATCAGTTTGGATAAGTCGCAAGGTGATGTGAAAAATAA
TTATGGTGCATTTTTATGTAGTCAGGGCGAATTTGCTCGTGCTTATGAGTTATTTGAGCAGGCACTTACTACGCCTAATT
ATTATCAGCAAGCGGATACCTATGCAAATATCATAATTTGTGCAGAGGCGGAACATAATATTCCACGTTATCAGAAATTT
TTACAGTTACTGAAGAATATTGATCCCAAAAGAGCGGAGGAAATAAAGAAAGTTTCTTAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_7)

(109-130)

Tetratricopeptide repeat (TPR_8)

(74-100)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

52.121

92.179

0.48
  pilF Glaesserella parasuis strain SC1401

44.068

98.883

0.436

Multiple sequence alignment