Detailed information NTG0030360

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: XFASM12_RS05865
• Genome accession: NC_010513
• Coordinates: 1321043..1322173 (+)
• Length: 376 a.a.
• NCBI ID: WP_004084950.1
• Uniprot ID: -
• Organism: Xylella fastidiosa M12
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1316043..1327173

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
XFASM12_RS05840 (Xfasm12_1247)	-	1316367..1317191 (+)	825	WP_021358442.1	glycosyltransferase family 2 protein	-
XFASM12_RS05850 (Xfasm12_1249)	-	1317808..1318635 (-)	828	WP_004084945.1	glycosyltransferase family 2 protein	-
XFASM12_RS05855	-	1319020..1319232 (-)	213	WP_021358439.1	hypothetical protein	-
XFASM12_RS05860 (Xfasm12_1253)	pilT	1319854..1320888 (+)	1035	WP_004084948.1	type IV pilus twitching motility protein PilT	Machinery gene
XFASM12_RS05865 (Xfasm12_1254)	pilU	1321043..1322173 (+)	1131	WP_004084950.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
XFASM12_RS05870 (Xfasm12_1255)	-	1322842..1323810 (-)	969	WP_004084952.1	YafY family protein	-
XFASM12_RS05875 (Xfasm12_1257)	-	1324593..1325402 (+)	810	WP_021358437.1	DUF481 domain-containing protein	-
XFASM12_RS05880 (Xfasm12_1258)	hemC	1325456..1326373 (-)	918	WP_004084955.1	hydroxymethylbilane synthase	-
XFASM12_RS05885 (Xfasm12_1259)	-	1326381..1327139 (-)	759	WP_004084957.1	LytTR family DNA-binding domain-containing protein	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 42402.63 Da
• Isoelectric Point: 6.3881

>NTDB_id=30360 XFASM12_RS05865 WP_004084950.1 1321043..1322173(+) (pilU) [Xylella fastidiosa M12]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECNFAIDITS
IGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVT
IEDPIEFIHKHERCIITQREIGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAI
DRIINFFPEERRSQLLIDLSLNLKGIIAQQLIPSPDGKERCLAAEVLRDSPLVQDYIRVGEIHKLKEVMQNPNDLGMTTF
DQSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDAKTLSQGFDGVEIAEMS

Nucleotide

• Length: 1131 bp

>NTDB_id=30360 XFASM12_RS05865 WP_004084950.1 1321043..1322173(+) (pilU) [Xylella fastidiosa M12]
ATGACTACCATCGATTTCACATCCTTACTTAAGCTGATGGCGCACCAAAAAGCGTCGGATTTATTTATTACCTGTGGAAT
GCCGCCTTCAATGAAGATTCACGGCAATATCACTCCAATCACACAAGTACCGCTGACTTCAGAGCAAAGCCGTGACATAG
TCTTCAATATCATGAGTGAAGCGCAGCGCGAGGAATTCGAGAAGGTCCACGAATGCAACTTCGCAATTGACATCACCAGC
ATTGGTCGCTTCCGTGTCAACGCTTTCTATCAGCGTAACCAAATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACCTTCGAAAAACTGAAGCTCCCGCAGGTCGCTAAGGAGCTCGTCATGGAGAAGCGCGGCATCATCATCATCGTGGGTG
CCACGGGTAGTGGTAAATCGACCTCAATGGCATCAATGATCAACCACCGTAATCACAACTCAACAGGACATATCGTCACT
ATCGAAGATCCCATTGAGTTTATTCACAAGCACGAGAGATGCATCATCACCCAACGCGAAATAGGTACCGACACCGACAG
TTGGGAAAATGCCTTGAAAAATACCCTACGCCAAGCCCCAGATGTGATTGTGATTGGTGAGGTGCGTACTCAAGAGACAA
TGGATCATGCCATCTCATTCTCCGAAACTGGCCATTTGGTCATGTGTACCCTACATGCCAACAACGCCAATCAAGCAATA
GACCGCATTATTAACTTCTTCCCGGAAGAGCGGCGCAGCCAATTACTGATAGATCTGTCGCTAAATCTAAAAGGCATCAT
CGCTCAACAATTGATCCCATCTCCGGATGGTAAAGAGCGCTGTTTAGCAGCGGAAGTTTTGCGCGACAGTCCATTAGTAC
AGGACTACATACGCGTAGGAGAGATCCATAAACTCAAAGAAGTCATGCAAAACCCCAACGATCTTGGAATGACGACATTC
GATCAAAGCTTGGTCGAACTCTATCATAGCGGAGCCATTAACTATAAAGAGGCACTACGCCATGCTGATTCGCAGAACGA
GGTACGCCTGCGTATTAAACTCTCCGATGGAAAAGACGCTAAAACTCTGAGTCAAGGATTCGATGGAGTGGAAATTGCAG
AAATGTCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-273)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.853	97.606	0.604
pilU	Acinetobacter baylyi ADP1	60.169	94.149	0.566
pilU	Vibrio cholerae strain A1552	51.404	94.681	0.487
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.484	96.809	0.402