Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   DN409_RS20340 Genome accession   NZ_CP031071
Coordinates   3989003..3989437 (-) Length   144 a.a.
NCBI ID   WP_002014817.1    Uniprot ID   -
Organism   Bacillus mycoides strain BPN401     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3984003..3994437
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DN409_RS20325 (DN409_20340) - 3985145..3985702 (+) 558 WP_002128898.1 PadR family transcriptional regulator -
  DN409_RS20330 (DN409_20345) metE 3986244..3988532 (+) 2289 WP_149190871.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  DN409_RS20335 (DN409_20350) comJ 3988574..3988963 (-) 390 WP_149190872.1 competence protein ComJ -
  DN409_RS20340 (DN409_20355) nucA/comI 3989003..3989437 (-) 435 WP_002014817.1 DNA-entry nuclease Machinery gene
  DN409_RS20345 (DN409_20360) - 3989674..3990417 (+) 744 WP_002033817.1 DUF3967 domain-containing protein -
  DN409_RS20350 (DN409_20365) - 3990477..3991751 (-) 1275 WP_002014814.1 HAMP domain-containing sensor histidine kinase -
  DN409_RS20355 (DN409_20370) - 3992061..3992261 (+) 201 WP_105584768.1 hypothetical protein -
  DN409_RS20360 (DN409_20375) - 3992496..3993659 (+) 1164 WP_149190873.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16194.59 Da        Isoelectric Point: 8.4331

>NTDB_id=303218 DN409_RS20340 WP_002014817.1 3989003..3989437(-) (nucA/comI) [Bacillus mycoides strain BPN401]
MKQFKGIIISIIAILSLLVAVYEVLVPEETSTKKTTTYDQILEFPKERYPETGKHITDAIKEGHSEMCTIDRNGAADRRK
LSLAPYPTKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=303218 DN409_RS20340 WP_002014817.1 3989003..3989437(-) (nucA/comI) [Bacillus mycoides strain BPN401]
ATGAAGCAATTTAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTCTTTTGGTAGCAGTATATGAAGTACTTGTGCC
AGAGGAGACAAGTACTAAAAAAACAACTACGTATGATCAAATTCTAGAGTTCCCGAAAGAACGATATCCAGAGACCGGGA
AACATATAACGGATGCTATAAAAGAAGGACATTCGGAAATGTGTACAATTGATCGTAATGGTGCAGCGGATAGAAGGAAA
TTATCGTTAGCGCCATATCCGACGAAGAAAGGTTATGATCGTGATGAATGGCCGATGGCGATGTGTAAGGAAGGCGGAAA
AGGAGCTCATATTGAATATATAAGTCCAGCAGATAACCGCGGTGCAGGTTCTTGGGTAGGGAATAAGCTAGACAAATATC
CAGATGGTACGCGTGTGAAATTTGAAGTGAAATAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(47-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.027

78.472

0.542

Multiple sequence alignment