Detailed information NTG0303201

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: DN409_RS05805
• Genome accession: NZ_CP031071
• Coordinates: 1130286..1134011 (+)
• Length: 1241 a.a.
• NCBI ID: WP_061688971.1
• Uniprot ID: -
• Organism: Bacillus mycoides strain BPN401
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1125286..1139011

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DN409_RS05790 (DN409_05785)	-	1125443..1126036 (+)	594	WP_016119803.1	TVP38/TMEM64 family protein	-
DN409_RS05795 (DN409_05790)	lepB	1126093..1126656 (+)	564	WP_016119804.1	signal peptidase I	-
DN409_RS05800 (DN409_05795)	addB	1126774..1130289 (+)	3516	WP_149190247.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
DN409_RS05805 (DN409_05800)	addA	1130286..1134011 (+)	3726	WP_061688971.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
DN409_RS05810 (DN409_05805)	-	1134035..1134313 (+)	279	WP_002011271.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
DN409_RS05815 (DN409_05810)	-	1134529..1135236 (+)	708	WP_060749458.1	DNA alkylation repair protein	-
DN409_RS05820 (DN409_05815)	gerPF	1135292..1135507 (-)	216	WP_001141570.1	spore germination protein GerPF	-
DN409_RS05825 (DN409_05820)	-	1135550..1135936 (-)	387	WP_002087037.1	spore germination protein GerPE	-
DN409_RS05830 (DN409_05825)	gerPD	1135951..1136145 (-)	195	WP_002087035.1	spore germination protein GerPD	-
DN409_RS05835 (DN409_05830)	-	1136152..1136766 (-)	615	WP_002087034.1	spore germination protein GerPC	-
DN409_RS05840 (DN409_05835)	gerPB	1136834..1137040 (-)	207	WP_001012506.1	spore germination protein GerPB	-
DN409_RS05845 (DN409_05840)	gerPA	1137055..1137276 (-)	222	WP_001111190.1	spore germination protein GerPA	-
DN409_RS05850 (DN409_05845)	-	1137373..1137552 (-)	180	WP_002087032.1	aspartyl-phosphate phosphatase Spo0E family protein	-
DN409_RS05855 (DN409_05850)	-	1137796..1138698 (+)	903	WP_002125896.1	fumarylacetoacetate hydrolase family protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142589.82 Da
• Isoelectric Point: 4.9021

>NTDB_id=303201 DN409_RS05805 WP_061688971.1 1130286..1134011(+) (addA) [Bacillus mycoides strain BPN401]
MIENWPQKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIISEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDGPGSQHIRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNSIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEKHI
RKATELAMLPDGPAPRVETLQADLALLGTLSSAARGSWTSVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRRPESFLRDFQDMHPVLEKLVKLVKVFTERFQAIKRDKGMVDFTDLEHFCLQILSEQSEEGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGSGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVQDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGNIPGEIYEYSASWK
VEVVEGKDLLAPEPVQEEKQELLEALRDKKAVPLESERKEEVYDRLMWEYGYADATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITIEVLQEQIARMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLSAEEAYQDWQGKKGESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFTGGFDQAKPIL
EDRYKVQLSLYAKALEKSLKHPVKEKCLYFFDGNHVVNIDE

Nucleotide

• Length: 3726 bp

>NTDB_id=303201 DN409_RS05805 WP_061688971.1 1130286..1134011(+) (addA) [Bacillus mycoides strain BPN401]
ATGATAGAAAATTGGCCTCAGAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCGGTTGTAGCGAACGGACG
TGATATTTTAGTCGCAGCGGCAGCTGGATCAGGGAAAACAGCGGTATTAGTTGAACGTATTATTAAAAAGATTATAAGTG
AGGAAAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCAGCGCAAGAAATGAAAAATCGAATT
GGGGAAGCGTTAGAAAAAGTATTAATTGATGGGCCAGGTTCACAGCATATAAGAAAGCAGCTTAGCTTATTAAATAAAGC
TTCCATTTCTACAATTCATTCATTTTGTTTACAAGTTATTAGAGGATATTATTACATGCTTGATGTCGATCCTCGTTTTC
GTATTGCGAACCAAACAGAAAATGAGTTATTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAATATGGAATCGAA
GATAATAGTATTTTCTTTGAACTAGTTGATCGTTATACGAGTGACCGTAGTGACGATGACTTACAACGAATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAACCCGGAGAAGTGGCTTGATAAATTAGTAGAAGCATACGACGTTGAAGGAA
AGACGATTGAAGATTTAGTGTACGCTTCTTACTTATTAGAAGATGTAAAATTCCAGCTTGAAACAGCGGAAAAGCATATT
CGTAAGGCGACTGAACTCGCAATGCTTCCTGATGGTCCAGCGCCTCGCGTTGAAACTTTACAAGCGGATTTAGCTTTACT
TGGAACGTTATCCTCAGCAGCTCGTGGATCGTGGACAAGCGTTTATGAAGCGATGCAAAATGTATCGTGGCAAACGTTAA
AGCGTATTAAAAAAAGTGATTACAACGAAGATGTTGTAAAACAAGTAGATTCTCTTCGTAATAAAGCGAAAGATGAGGTG
AAGAAATTACAAGAAGAGCTATTTAGCCGCAGACCCGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAACTCGTGAAGCTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATTAAGCGAGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTGCAAATTTTAAGCGAGCAAAGTGAAGAGGGTGAAATGAAGCCGTCAGCAGTAGCACTTCAA
TATCGTAATAAATTTGCTGAAGTACTAGTTGATGAATATCAAGATACGAACTTCGTACAGGAATCAATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTGTTCATGGTTGGTGACGTAAAACAGTCAATCTATCGTTTCCGACTAGCAG
AGCCTGGTTTATTCTTAGGAAAATATAAACGTTTCACACAAGAAGGATCGGGCGGCGGAATGAAGATTGACTTAGCGAAA
AACTTCCGTAGCCGTCATGAAGTGCTAGCAGGTACGAACTTTATTTTCAAACAAATTATGGGCGAAGAAGTCGGCGAAAT
CGATTATGATGCTGACGCTGAATTAAAGCTAGGTGCTAGCTATCCAGAAGGTGAAGATGTAGCGGCAGAGCTATTATGTA
TTCAGCAAACTGAGGAAGAAGTGCAAGACGGTGAAGAAGGTGCCGAAGTAGAAAAAGCGCAGCTTGAAGCTCGTCTTATG
GCGCAGCGTATTAAAGCAATGGTCGATTCAGGCTATGAAGTGTATGATCGTAAAACTGATAGTATGCGCCCAGTGCAATA
TCGTGATTTCGTTATTTTACTTCGCTCCATGCCATGGGCGCCGCAAATTATGGAAGAGTTAAAACTACAAGGAATTCCGG
TATATGCTGACCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTCATTGATAAC
CCGATGCAAGATATTCCGCTTGCAGCTGTACTTCGTTCACCGATCGTTGGACTAAATGACGAAGAACTTGCGACGCTTCG
TGCTCATGGAAAGAAAGGCTCGTTTTATGAAGTGATGAGCTCATTCTTAAAGGGAGCGCCGCTTGAAGAAGAACAAGAAC
TGCATGATAAATTAGAGTGGTTTTATAACTTACTGCAAGGGTGGCGTGAATTTGCGCGTCAACAATCTCTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGCTATTACGACTTTGTCGGTGGTTTACCAGCCGGAAAGCAAAGGCAGGCAAACTT
ACGCGTACTATATGACCGTGCAAGACAATATGAAGCAACATCGTTTAGAGGATTATTCCGCTTCTTGCGCTTTATTGAGC
GTATTTTAGAACGCGGAGATGATATGGGAACGGCGAGAGCCCTCGGAGAACAAGAAGATGTTGTTCGCATTATGACGATT
CATAAAAGTAAAGGGCTAGAGTTCCCAGTCGTATTTGTAGCTGGACTTGGTCGCCGTTTTAATACACAAGATTTAATGAA
GCGTTTCTTACTTCATAAAGATTTCGGTTTCGGTTCACAATTTATCGATCCTCGTAAACGAATTAAATATACGACATTAT
CGCAACTAGCGATTAAGCGTAAAATGAAGATGGAATTAATTGCGGAAGAAATGCGCGTACTATACGTAGCATTAACACGT
GCAAAAGAGAAGTTGATTTTAATTGGAACAGTTAAAGATGCAAATAAAGAAATGGAAAAATGGCTCGATGCAAGAGAGCA
TAGTGAATGGTTATTACCAGACCATATTCGTGCCGGAGCGTCTTGCTACTTAGACTGGATTGCACCTTCCTTATATAGAC
ACCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGAAATATTCCAGGCGAAATTTATGAATATAGTGCTAGCTGGAAA
GTAGAAGTTGTTGAAGGGAAAGATTTACTTGCACCAGAACCAGTTCAAGAAGAGAAACAAGAATTATTAGAAGCGCTTCG
TGATAAAAAAGCTGTTCCGTTAGAAAGTGAACGGAAAGAAGAAGTGTACGACAGATTGATGTGGGAATACGGGTATGCGG
ACGCGACATCTCACCGTGCGAAGCAGTCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAACGCC
TTTATTAAAAAACTTCGTGCACCAATTAAAACACGTCCGCGCTTTATGGAGAAAAAAGGGCTAACATACGCAGAGCGAGG
GACAGCAGTCCATGCCGTTATGCAGCATGTAGATTTGAAGAAACCAATTACGATTGAAGTTCTTCAAGAACAAATTGCAA
GAATGGTAAATAAAGAACTATTAACATTTGAACAAGCTGAAGAAATAGCGATTGAAAAGGTAATTTCATTCTTTGACAGT
GACTTAGGTAAAAGGGTATTAGCAGCAAAAAGTGTTGAGCGCGAAGTACCATTTACGATGATGCTTTCAGCAGAAGAAGC
GTATCAAGATTGGCAAGGGAAGAAAGGCGAATCAATACTTGTCCAAGGGGTTATCGACTGCATGATCGAAGAGGAAGACG
GAATTACTTTAATCGACTTTAAAACAGATACGATTGAAGGAAAGTTCACAGGCGGATTTGATCAAGCGAAACCAATTTTA
GAAGACCGATACAAAGTACAGCTTTCACTATATGCAAAAGCACTCGAGAAAAGCTTAAAACATCCTGTGAAAGAGAAATG
TTTATATTTCTTTGATGGAAATCATGTTGTAAATATTGACGAATAG

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1060-1231)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.569	100	0.538