Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   DN409_RS05800 Genome accession   NZ_CP031071
Coordinates   1126774..1130289 (+) Length   1171 a.a.
NCBI ID   WP_149190247.1    Uniprot ID   -
Organism   Bacillus mycoides strain BPN401     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1121774..1135289
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DN409_RS05760 (DN409_05755) cspA 1122508..1122711 (+) 204 WP_002011282.1 RNA chaperone/antiterminator CspA -
  DN409_RS31250 (DN409_05760) - 1122738..1122999 (+) 262 Protein_1024 hypothetical protein -
  DN409_RS05770 (DN409_05765) - 1123050..1123235 (-) 186 WP_002011281.1 hypothetical protein -
  DN409_RS31885 (DN409_05770) - 1123529..1123615 (+) 87 Protein_1026 RNA-guided endonuclease TnpB family protein -
  DN409_RS05780 (DN409_05775) - 1123665..1124246 (+) 582 WP_002011279.1 competence protein ComK -
  DN409_RS05785 (DN409_05780) - 1124493..1125088 (+) 596 Protein_1028 DUF4306 domain-containing protein -
  DN409_RS05790 (DN409_05785) - 1125443..1126036 (+) 594 WP_016119803.1 TVP38/TMEM64 family protein -
  DN409_RS05795 (DN409_05790) lepB 1126093..1126656 (+) 564 WP_016119804.1 signal peptidase I -
  DN409_RS05800 (DN409_05795) addB 1126774..1130289 (+) 3516 WP_149190247.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  DN409_RS05805 (DN409_05800) addA 1130286..1134011 (+) 3726 WP_061688971.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  DN409_RS05810 (DN409_05805) - 1134035..1134313 (+) 279 WP_002011271.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  DN409_RS05815 (DN409_05810) - 1134529..1135236 (+) 708 WP_060749458.1 DNA alkylation repair protein -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134440.41 Da        Isoelectric Point: 5.1496

>NTDB_id=303200 DN409_RS05800 WP_149190247.1 1126774..1130289(+) (addB) [Bacillus mycoides strain BPN401]
MSLRFVIGRAGSGKSTLCLHEVQEELKQRPRGKTILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDTHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLIEKLSDSEYVKGAEIYIDGFHSFSPQELEIVRGLMRLGTRVTITLTIDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKTIPLMEQPRFHSQALAHLETHYEARPNEKYHGEASVTISTAANL
RAEVEGVAREIRRLVADEDYRYRDVAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLNVRKEAMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDASEMITDSEREMEERINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEELRVRAEENGDFLFATDHEQVWEEVMNLLDTFVEMLGEEK
MSLSMFTEVMSTGLEALQFANIPPSLDQVLVANIDHSRLSDIKATFIIGVNEGVIPAAPMDEGMLSDEERNVLTAAGIEL
APTTRQTLLEEQFVMYQMVTRASEKLYISCPLADEEGKTLLASSFIKKIKRMFPDVKESFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTDVSRDLYGDTIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKRIADKLLRENRTWADLSIKECEHLSALVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSLILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFALPNGVKMEVVGRIDRVDKAEDESG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAHTWMKKGSTASPAGVLYFHIHNPIVEMKGDASEEEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSVRSSIASEQEFNVLQKYVHHSFENIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=303200 DN409_RS05800 WP_149190247.1 1126774..1130289(+) (addB) [Bacillus mycoides strain BPN401]
ATGTCACTTCGATTTGTGATTGGTAGAGCTGGAAGTGGAAAAAGTACACTTTGTTTACACGAAGTGCAAGAAGAGTTAAA
ACAGCGCCCAAGAGGGAAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAAACGCAGCAGGCGTTAATTGGGA
GCGAAGATGTAAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCGCGATTAGCGTGGAAGGTGCTGCAAGAAGTTGGC
GGAGCGAGTCGTCTTCATATTGATGAAGCTGGTGTACATATGTTGCTTCGTAAAATTGTAGAATCTCGTAAAGATGGATT
ATCGGTGTTTCAAAAAGCAGCGGAGCAAAACGGTTTCTTTGAACATCTTGGTAGTATGATTGCTGAGTTTAAACGTTACA
ATGTGACGCCATCTAACGTATATGAAATGTGGCAACAATTAGACACGCATAGCAGTAGTGCAGAGCAAAAGCTGCTAGCG
AATAAAGTGTATGATTTACAGTTATTATATGATGATTTCGAACGTGCTTTAATCGGAAAATATTTAGATTCAGAAGACTA
CTTACAATTATTAATTGAGAAGCTTTCAGACTCTGAGTATGTAAAAGGCGCGGAAATTTATATAGATGGATTCCATTCAT
TTTCTCCTCAAGAGCTAGAAATCGTTAGAGGGCTAATGAGATTAGGAACTAGAGTAACAATAACATTAACGATAGATGAA
AAAACGTTAGCGCAGCCAGTGAATGAACTAGATTTATTTTATGAAACGACGTTAACGTATGAAAAAATAAAACAAGTAGC
GCGTGAAGAGAAAATAGAAATTGAAAAAACAATTCCGCTGATGGAACAGCCGCGTTTTCATTCGCAAGCATTAGCACATT
TAGAAACGCACTACGAAGCACGTCCGAATGAAAAGTATCATGGTGAAGCGAGTGTGACAATCAGTACAGCAGCTAATTTA
CGAGCTGAAGTAGAAGGTGTAGCCCGTGAAATTAGAAGGCTTGTGGCGGATGAAGACTATCGTTACCGAGATGTCGCAGT
CCTTCTTCGTAACGGAGAAAGTTATTACGACGTAATGCGAACGTTATTTACGGATTATAATATCCCGCATTTCATCGATG
AAAAACGCCCAATGTCACATCATCCGTTAGTAGAATGCATTCGTTCTGCACTCGAGATTATTAGCGGGAATTGGCGTTAT
GATGCAGTATTTCGCTGCGTGAAAACAGAGCTTTTATATCCATTAAATGTAAGAAAAGAAGCGATGCGCGAAGAGATGGA
TGAGTTTGAAAACTACTGTTTAGCGTACGGTGTACAAGGGAAGAGATGGACTTCTGAAGATCCGTGGATGTATCGTCGTT
ATCGTTCTCTTGATGATGCGAGCGAAATGATAACAGACAGTGAGCGTGAAATGGAAGAGAGAATAAATCGACTTCGCGAC
GTTGTAAGAACGCCAGTTATTCGTATGCAAAAAAGACTGAAGCGTGCTGGAACAGTTATGCAAATGTGCGAAGCGGTATA
TTTATTTTTAGAGGAGCTTGACGTTCCGAAAAAACTAGAAGAATTACGTGTTCGTGCAGAAGAAAATGGAGATTTCTTAT
TTGCGACAGACCATGAGCAAGTATGGGAAGAAGTGATGAATCTTCTTGATACGTTCGTTGAGATGCTTGGCGAAGAGAAG
ATGTCCCTTTCAATGTTTACGGAGGTTATGTCGACAGGTCTTGAGGCACTTCAATTTGCGAACATTCCGCCGTCACTAGA
TCAAGTGTTAGTTGCTAATATTGATCATTCTAGATTATCAGATATTAAAGCGACGTTCATTATTGGAGTAAATGAAGGAG
TGATTCCAGCAGCGCCTATGGATGAAGGTATGCTTTCTGATGAGGAAAGAAATGTTCTCACCGCTGCAGGTATCGAATTA
GCACCAACGACGAGACAAACTTTATTAGAAGAACAGTTCGTTATGTATCAAATGGTGACGAGAGCATCTGAGAAGTTATA
CATTTCCTGTCCGCTTGCGGATGAAGAAGGAAAGACGTTACTTGCTTCTAGTTTTATTAAAAAAATAAAAAGAATGTTCC
CTGATGTGAAAGAATCGTTTATTACGAATGATGTAAATGACTTATCGCGTTCGGAACAAATCTCATATGTAGCAACGCCA
GAAGTAACGTTGTCTTACGTCATGCAGCAACTGCAAACGTGGAAGCGATACGGATTTGAAGGGAATTTAGACTTTTGGTG
GGACGTATATAACTTCTACGTTACTTCAGATGAATGGAAGCAAAAAAGTAGCCGTGTGTTATCAAGTTTATTCTACCGAA
ATCGTGCACAGAAACTAAGTACAGATGTAAGTAGGGATTTATATGGAGATACAATTAAGGGAAGCGTTTCTCGTATGGAA
CTATTTAATCGCTGTGCGTACGCTCATTTTGCCCAGCACGGTTTATCGCTAAGAGAGCGTGATATTTTCAAACTTGATGC
GCCAGATATCGGTGAATTATTCCATGCAGCGCTGAAGAGAATTGCAGACAAGCTATTACGTGAAAATCGGACTTGGGCGG
ATTTATCAATAAAAGAGTGTGAACACCTTTCTGCTTTAGTAATAGAAGAAATTGCACCGTTATTACAAAGACAAATTTTA
TTAAGTTCGAACCGTCATTTCTATTTAAAGCAAAAACTACAACAAATCATTTTCCGTACGTCACTTATTCTTCGTGAACA
TGCGAAGTCGAGTGGATTTGTACCAGTTGATTTAGAAGTACCGTTCGGTATGGGCGGGACTGGATCTCTTCCGCCAATGG
AATTTGCGTTGCCAAATGGTGTGAAGATGGAAGTAGTTGGCCGTATTGACCGTGTTGATAAGGCGGAAGATGAGAGTGGT
ACGTTCCTTCGTATTATTGATTATAAATCAAGTTCGAAAGCGTTAGATTTAACAGAAGTGTATTACGGACTCGCACTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCAAATGCACATACGTGGATGAAAAAGGGCAGTACTGCATCACCGGCGGGTG
TATTGTATTTCCATATTCATAACCCGATTGTTGAGATGAAAGGCGATGCATCTGAAGAAGAAATTGAGAAAGAAATTTTA
AAGAAATTTAAAATGAAAGGTCTCGTACTAGGAGATGCTGACGTTGTGCGTTTAATGGATAATAAACTTTCAACAGGAAG
CTCTGATATTATTTCTGCTGGTCTGAAAAAAGATGGTAGTTTTAGTGTGCGTTCTAGCATTGCAAGTGAACAAGAGTTTA
ACGTACTTCAAAAATATGTACACCACTCGTTTGAAAATATCGGAAAAGACATTACAGAAGGTGTTATCGATATTGCTCCT
TACAAAAAGGGGAATAAAGCGGCATGTACGTTCTGTAACTTCAAATCTGTTTGTCAATTTGATGAATCACTTGAAGATAA
CCAATTCCGTACGTTAAAAGATATGAAAGATAGCGAAGCGATGGAGAAAATTAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)

UvrD-like helicase C-terminal domain (UvrD_C)

(301-669)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.319

100

0.494

Multiple sequence alignment