Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DN409_RS00535 Genome accession   NZ_CP031071
Coordinates   97705..99081 (+) Length   458 a.a.
NCBI ID   WP_002169331.1    Uniprot ID   R8NPL5
Organism   Bacillus mycoides strain BPN401     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 92705..104081
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DN409_RS00515 (DN409_00515) ctsR 92904..93365 (+) 462 WP_002029353.1 transcriptional regulator CtsR -
  DN409_RS00520 (DN409_00520) - 93535..94083 (+) 549 WP_002009823.1 UvrB/UvrC motif-containing protein -
  DN409_RS00525 (DN409_00525) - 94088..95152 (+) 1065 WP_002169332.1 protein arginine kinase -
  DN409_RS00530 (DN409_00530) clpC 95175..97610 (+) 2436 WP_002009819.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  DN409_RS00535 (DN409_00535) radA 97705..99081 (+) 1377 WP_002169331.1 DNA repair protein RadA Machinery gene
  DN409_RS00540 (DN409_00540) disA 99085..100158 (+) 1074 WP_000392162.1 DNA integrity scanning diadenylate cyclase DisA -
  DN409_RS00545 (DN409_00545) - 100319..101422 (+) 1104 WP_002063406.1 PIN/TRAM domain-containing protein -
  DN409_RS00550 (DN409_00550) ispD 101439..102119 (+) 681 WP_061689411.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  DN409_RS00555 (DN409_00555) ispF 102233..102709 (+) 477 WP_061689410.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49854.60 Da        Isoelectric Point: 7.4250

>NTDB_id=303193 DN409_RS00535 WP_002169331.1 97705..99081(+) (radA) [Bacillus mycoides strain BPN401]
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQSEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSLYDVLYISGEESAKQIKLRADRLHVKGSNLFVVAETDLQRIA
AHIEEMNPAFVVIDSIQTIHLPEVTSAPGSVTQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKATAPADAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=303193 DN409_RS00535 WP_002169331.1 97705..99081(+) (radA) [Bacillus mycoides strain BPN401]
ATGGCTAAAAAGAAAACAAAATTTACATGTCAAGAATGTGGTTATCAGTCGCCAAAATATATGGGGAAATGTCCTGGATG
TGGTCAATGGAATACGCTTGTTGAAGAGATGGAACCAGTTGTATCATCAAGACGCCTTAATTATGCCAATGCAATTCAAT
CAGAAGTAACAAAACCAAGACGCTTAACAGAGGTAGAAACAAAGTCTGAGGCGCGTATTGAAACGAAATTTCAAGAGTTT
AACCGTGTACTTGGTGGCGGGATTGTAGATGGATCTTTAGTACTTATTGGTGGAGACCCTGGGATTGGGAAATCAACATT
GTTATTACAAATTTCATCGCAATTAGCAGATTCTTTATATGATGTACTATACATATCAGGTGAGGAGTCAGCAAAGCAGA
TCAAACTTCGTGCAGATCGTTTGCATGTAAAGGGTAGTAATCTATTTGTTGTAGCAGAGACTGATTTACAGAGAATTGCA
GCGCACATTGAAGAGATGAATCCAGCTTTTGTTGTTATTGACTCCATCCAGACGATACATTTACCTGAAGTGACGTCAGC
CCCAGGGAGTGTAACACAAGTACGTGAATGTACAGCGGAATTAATGAAACTTGCAAAGACGAAAGGGATTCCTATTTTTA
TCGTCGGACATGTGACAAAAGAAGGGGCAATTGCAGGACCACGTATGCTAGAACATATGGTTGATGCAGTTCTTTACTTT
GAAGGAGATCGTCACCATACATATCGTATTTTGCGAGCTGTGAAGAACCGTTTTGGTTCCACAAATGAAATGGGTATCTT
TGAAATGAAGGAACTAGGCCTTGCAGAAGTACTAAATCCTTCTGAAATTTTCCTTGAGGAACGTCCAGTTGGAGTTGCAG
GATCCACAGTGGTTGCTTCAATGGAAGGAACAAGACCGGTTTTAGTAGAAATACAAGCATTAATCTCCCCTACTAGTTTT
GGAAATCCTCGAAGAATGGCGACGGGAATTGATCATAACCGCGTTTCACTTATTATGGCAGTGCTAGAAAAAAGAACAGG
TTTATTGTTGCAAAATCAAGACGCATATTTAAAAGTTGCGGGTGGCTTGAAATTAGATGAACCAGCAATCGATTTAGCTG
TCGCCTTAAGTATTGCTTCAAGTTTCAGAGATAAAGCTACTGCACCAGCCGATGCGGTAATAGGAGAAGTAGGCCTAACT
GGAGAAATAAGAAGAGTATCAAGAATTGAACAACGTGTACAAGAAGCAGCGAAATTAGGATTTCAACGTGCTATTATTCC
TAGAAAAAATTTGGGAGGATGGACAATTCCAGATGGGATTGAGGTAGTAGGTGTTTCTAATTTAGGAGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(60-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB R8NPL5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.51

100

0.747
  radA Streptococcus pneumoniae Rx1

64.018

98.908

0.633
  radA Streptococcus pneumoniae D39

64.018

98.908

0.633
  radA Streptococcus pneumoniae R6

64.018

98.908

0.633
  radA Streptococcus pneumoniae TIGR4

64.018

98.908

0.633
  radA Streptococcus mitis SK321

64.018

98.908

0.633
  radA Streptococcus mitis NCTC 12261

63.797

98.908

0.631

Multiple sequence alignment