Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   SYNPCC7002_RS03485 Genome accession   NC_010475
Coordinates   726189..728210 (+) Length   673 a.a.
NCBI ID   WP_012306325.1    Uniprot ID   A0AAE8IT23
Organism   Picosynechococcus sp. PCC 7002     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 721189..733210
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SYNPCC7002_RS03465 (SYNPCC7002_A0692) - 721449..721703 (-) 255 WP_012306321.1 sulfurtransferase TusA family protein -
  SYNPCC7002_RS03470 (SYNPCC7002_A0693) dnaJ 721700..722836 (-) 1137 WP_012306322.1 molecular chaperone DnaJ -
  SYNPCC7002_RS03475 (SYNPCC7002_A0694) dnaK 722958..724913 (-) 1956 WP_012306323.1 molecular chaperone DnaK -
  SYNPCC7002_RS03480 (SYNPCC7002_A0695) grpE 725030..725779 (-) 750 WP_012306324.1 nucleotide exchange factor GrpE -
  SYNPCC7002_RS03485 (SYNPCC7002_A0696) pilB/pilB1 726189..728210 (+) 2022 WP_012306325.1 GspE/PulE family protein Machinery gene
  SYNPCC7002_RS03490 (SYNPCC7002_A0697) pilT 728330..729436 (+) 1107 WP_012306326.1 type IV pilus twitching motility protein PilT Machinery gene
  SYNPCC7002_RS03495 (SYNPCC7002_A0699) - 729503..730741 (+) 1239 WP_012306328.1 type II secretion system F family protein -
  SYNPCC7002_RS03500 (SYNPCC7002_A0700) - 730750..731307 (+) 558 WP_012306329.1 hypothetical protein -
  SYNPCC7002_RS03505 (SYNPCC7002_A0701) - 731304..732380 (-) 1077 WP_012306330.1 hypothetical protein -

Sequence

Protein


Download         Length: 673 a.a.        Molecular weight: 75429.53 Da        Isoelectric Point: 5.2500

>NTDB_id=30244 SYNPCC7002_RS03485 WP_012306325.1 726189..728210(+) (pilB/pilB1) [Picosynechococcus sp. PCC 7002]
MTHSSLPKRTRALALRNDFSPFGNKLIQQGVVDHQQMEKALVETRRSGRPLTAIVEELTGKALSPELVRQYKKHHLFELK
ILYGLNSIDPEVQKIDIDQMGELINSLVPVELCRRHRLLPIRRVQDEVIVAMVEPDNLAAQDDLNRLFRLQNLTLRRIVI
TEDDYRQVLDGYLEQQVQIQIQQQEQAAKAAEAAELKKAVDIEDLDLGVAGELDEQEEESADLMNENQASSAPVIKLVNK
VLLKALQEGASDIHIEPQENDLRIRFRKDGVLQYAFDPLPKRIVPAVTARFKIMADLDIAERRVPQDGRIRRMFKGRKVD
FRVNSLPSRYGEKIVLRILDNSSTQLGLDLLITNQNILGQVREMAQRPFGLILVTGPTGSGKSTTLYSILAERNEPGINI
STAEDPIEYTLPGITQVQVIREKGMDFASILRAFLRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIA
RLDEMGVEPFMISGSLLGVLAQRLMRRVCGECRIPYQPTVEELARYGLSSNDRHPLTVYRANTLTPEQIQMAKEAGSLCP
KCGGGGYKGRVGVYEFMQNSEEIERLINTGATTDMIKEVAVQEGMLSILAYSLELVKEGHTTFEEVDRVTFTDSGLEAEL
KAKRKTGLVCDTCNAELQPDWLDCPYCMTPRFS

Nucleotide


Download         Length: 2022 bp        

>NTDB_id=30244 SYNPCC7002_RS03485 WP_012306325.1 726189..728210(+) (pilB/pilB1) [Picosynechococcus sp. PCC 7002]
ATGACCCACTCTTCCCTTCCCAAACGGACAAGAGCCCTTGCCCTCCGCAATGATTTCTCTCCTTTCGGCAACAAGCTCAT
TCAACAGGGAGTTGTAGACCATCAACAGATGGAAAAAGCCCTCGTTGAAACCCGGCGTAGTGGACGCCCCCTCACGGCCA
TTGTCGAAGAACTAACCGGGAAAGCCCTGTCCCCTGAGCTAGTCCGACAGTACAAAAAGCATCATCTCTTTGAACTGAAG
ATCCTCTACGGTCTCAATTCCATTGACCCAGAAGTTCAAAAAATTGACATCGATCAGATGGGCGAGTTGATCAATTCCCT
CGTGCCCGTAGAATTATGTCGCCGCCACCGTCTCCTTCCGATCCGGCGGGTTCAAGATGAAGTGATTGTGGCGATGGTAG
AGCCCGATAACCTAGCCGCCCAAGATGATTTAAACCGTCTTTTTCGCCTGCAAAATCTGACGTTACGGCGCATCGTGATC
ACAGAAGACGACTATCGTCAGGTGCTAGATGGCTATTTAGAACAGCAAGTCCAGATTCAAATCCAACAGCAGGAACAAGC
AGCCAAGGCCGCCGAAGCAGCAGAACTGAAAAAAGCAGTTGATATCGAAGATCTTGATCTCGGTGTAGCCGGGGAATTAG
ACGAGCAGGAAGAAGAAAGCGCCGACCTGATGAATGAAAATCAGGCTAGCTCTGCTCCGGTGATCAAACTTGTCAATAAA
GTGCTACTTAAAGCTCTCCAGGAAGGGGCCTCAGATATTCACATCGAACCCCAAGAAAATGATCTGCGGATTCGTTTCCG
TAAAGATGGGGTTCTGCAATATGCTTTTGATCCGCTGCCCAAACGGATTGTGCCGGCGGTAACGGCCCGGTTTAAAATTA
TGGCGGACCTAGACATTGCGGAACGTCGTGTACCTCAGGATGGTCGGATTCGGCGGATGTTTAAGGGGCGCAAGGTGGAC
TTTCGGGTCAATAGCTTACCCAGTCGCTACGGCGAAAAAATTGTTTTACGGATTCTCGATAATTCTTCAACCCAGTTGGG
CTTGGATTTGTTAATTACCAACCAAAATATTCTGGGGCAGGTGCGGGAAATGGCCCAGCGGCCATTTGGTCTAATTTTGG
TGACGGGGCCAACAGGCTCAGGAAAATCAACAACTTTGTATTCAATTCTGGCGGAGCGCAACGAGCCGGGCATCAACATC
AGTACCGCAGAAGACCCCATTGAATATACTTTGCCGGGCATTACCCAGGTGCAGGTCATCCGGGAAAAGGGGATGGATTT
TGCGTCAATTTTGCGGGCATTTTTGCGGCAGGATCCAGATGTCATTCTTGTGGGAGAAACCCGGGATAAGGAAACGGCAA
AAACAGCCATTGAGGCGGCTTTAACGGGGCACTTGGTACTAACGACTCTCCACACCAATGATGCTCCAGGGGCGATCGCC
CGTCTAGATGAGATGGGAGTTGAACCCTTTATGATTTCCGGGTCACTCCTGGGAGTTCTGGCCCAGCGCTTGATGCGACG
GGTTTGTGGGGAATGCCGCATCCCCTATCAGCCCACAGTGGAAGAGCTGGCACGCTACGGCCTTTCCAGTAATGATCGCC
ATCCTTTAACGGTGTACCGGGCCAATACCCTGACGCCAGAGCAAATCCAAATGGCCAAAGAAGCCGGCAGTCTCTGTCCT
AAATGTGGCGGCGGCGGCTACAAAGGCCGGGTGGGGGTCTATGAGTTTATGCAAAATAGTGAAGAAATTGAACGGTTGAT
TAATACCGGGGCCACCACGGACATGATCAAAGAAGTGGCAGTGCAAGAGGGCATGTTGAGTATCTTGGCCTACAGTTTGG
AGTTGGTCAAAGAAGGCCATACGACCTTTGAGGAGGTAGACCGTGTTACCTTTACTGATTCTGGCCTTGAGGCAGAACTA
AAGGCGAAACGAAAGACGGGCTTGGTTTGTGACACCTGTAATGCTGAGTTGCAGCCGGATTGGCTAGATTGTCCCTACTG
TATGACGCCCCGCTTTAGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(240-507)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

65.385

100

0.657

Multiple sequence alignment