Detailed information NTG0302106

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: Y31_RS06335
• Genome accession: NZ_CP030910
• Coordinates: 1286215..1287087 (-)
• Length: 290 a.a.
• NCBI ID: WP_003107301.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain Y31
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1281215..1292087

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Y31_RS06290 (Y31_1201)	-	1282076..1282246 (-)	171	WP_003094672.1	DUF3094 family protein	-
Y31_RS06295 (Y31_1202)	-	1282272..1282721 (-)	450	WP_003094670.1	MOSC domain-containing protein	-
Y31_RS06300 (Y31_1203)	-	1282718..1283347 (-)	630	WP_003094668.1	DUF1780 domain-containing protein	-
Y31_RS06305 (Y31_1204)	-	1283480..1283905 (+)	426	WP_003103865.1	GNAT family N-acetyltransferase	-
Y31_RS06310 (Y31_1205)	-	1283902..1284372 (+)	471	WP_115759333.1	FAD/FMN-containing dehydrogenase	-
Y31_RS06315 (Y31_1206)	-	1284369..1285058 (+)	690	WP_003103868.1	energy-coupling factor ABC transporter permease	-
Y31_RS06320 (Y31_1207)	-	1285164..1285373 (+)	210	WP_003094660.1	hypothetical protein	-
Y31_RS06325 (Y31_1208)	yacG	1285410..1285610 (-)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
Y31_RS06330 (Y31_1209)	coaE	1285607..1286218 (-)	612	WP_003094654.1	dephospho-CoA kinase	-
Y31_RS06335 (Y31_1210)	pilD	1286215..1287087 (-)	873	WP_003107301.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
Y31_RS06340 (Y31_1211)	pilC	1287088..1288305 (-)	1218	WP_003107299.1	type II secretion system F family protein	Machinery gene
Y31_RS06345 (Y31_1212)	pilB	1288309..1290009 (-)	1701	WP_003122078.1	type IV-A pilus assembly ATPase PilB	Machinery gene
Y31_RS06350 (Y31_1213)	-	1290240..1290704 (+)	465	WP_003122079.1	pilin	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31873.90 Da
• Isoelectric Point: 7.7068

>NTDB_id=302106 Y31_RS06335 WP_003107301.1 1286215..1287087(-) (pilD) [Pseudomonas aeruginosa strain Y31]
MTLLDYLASHPLAFVLCTILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide

• Length: 873 bp

>NTDB_id=302106 Y31_RS06335 WP_003107301.1 1286215..1287087(-) (pilD) [Pseudomonas aeruginosa strain Y31]
ATGACCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCACCATCCTCCTCGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAAGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTAGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGTCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.357	96.552	0.534
pilD	Vibrio campbellii strain DS40M4	52.65	97.586	0.514
pilD	Acinetobacter nosocomialis M2	50.53	97.586	0.493
pilD	Acinetobacter baumannii D1279779	50.53	97.586	0.493
pilD	Neisseria gonorrhoeae MS11	48.921	95.862	0.469