Detailed information NTG0301922

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: CWE28_RS18285
• Genome accession: NZ_CP030861
• Coordinates: 3787335..3788207 (-)
• Length: 290 a.a.
• NCBI ID: WP_003107301.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain HS9
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3782335..3793207

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CWE28_RS18240 (CWE28_18235)	-	3783196..3783366 (-)	171	WP_003094672.1	DUF3094 family protein	-
CWE28_RS18245 (CWE28_18240)	-	3783392..3783841 (-)	450	WP_003161439.1	MOSC domain-containing protein	-
CWE28_RS18250 (CWE28_18245)	-	3783838..3784467 (-)	630	WP_003120948.1	DUF1780 domain-containing protein	-
CWE28_RS18255 (CWE28_18250)	-	3784600..3785025 (+)	426	WP_003103865.1	GNAT family N-acetyltransferase	-
CWE28_RS18260 (CWE28_18255)	-	3785022..3785492 (+)	471	WP_019486073.1	hypothetical protein	-
CWE28_RS18265 (CWE28_18260)	-	3785489..3786178 (+)	690	WP_003103868.1	energy-coupling factor ABC transporter permease	-
CWE28_RS18270 (CWE28_18265)	-	3786284..3786493 (+)	210	WP_003094660.1	hypothetical protein	-
CWE28_RS18275 (CWE28_18270)	yacG	3786530..3786730 (-)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
CWE28_RS18280 (CWE28_18275)	coaE	3786727..3787338 (-)	612	WP_023114838.1	dephospho-CoA kinase	-
CWE28_RS18285 (CWE28_18280)	pilD	3787335..3788207 (-)	873	WP_003107301.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
CWE28_RS18290 (CWE28_18285)	pilC	3788208..3789425 (-)	1218	WP_003161763.1	type II secretion system F family protein	Machinery gene
CWE28_RS18295 (CWE28_18290)	pilB	3789429..3791129 (-)	1701	WP_021205142.1	type IV-A pilus assembly ATPase PilB	Machinery gene
CWE28_RS18300 (CWE28_18295)	-	3791360..3791824 (+)	465	WP_021205141.1	pilin	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31873.90 Da
• Isoelectric Point: 7.7068

>NTDB_id=301922 CWE28_RS18285 WP_003107301.1 3787335..3788207(-) (pilD) [Pseudomonas aeruginosa strain HS9]
MTLLDYLASHPLAFVLCTILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide

• Length: 873 bp

>NTDB_id=301922 CWE28_RS18285 WP_003107301.1 3787335..3788207(-) (pilD) [Pseudomonas aeruginosa strain HS9]
ATGACCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCACCATCCTCCTCGGCCTGCTGGTCGGCAG
CTTCCTCAATGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAGGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGTCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCTAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.357	96.552	0.534
pilD	Vibrio campbellii strain DS40M4	52.65	97.586	0.514
pilD	Acinetobacter nosocomialis M2	50.53	97.586	0.493
pilD	Acinetobacter baumannii D1279779	50.53	97.586	0.493
pilD	Neisseria gonorrhoeae MS11	48.921	95.862	0.469