Detailed information NTG0301731

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: DRA66_RS07745
• Genome accession: NZ_CP030825
• Coordinates: 1549274..1550134 (+)
• Length: 286 a.a.
• NCBI ID: WP_002241067.1
• Uniprot ID: A1KVV2
• Organism: Neisseria meningitidis strain 12-208
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1544274..1555134

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DRA66_RS07725 (DRA66_09110)	dapD	1544784..1545605 (-)	822	WP_002220281.1	2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase	-
DRA66_RS07730 (DRA66_09115)	-	1545615..1545845 (-)	231	WP_025462060.1	hypothetical protein	-
DRA66_RS07735 (DRA66_09120)	pgi	1545815..1547458 (-)	1644	WP_002247959.1	glucose-6-phosphate isomerase	-
DRA66_RS07740 (DRA66_09130)	pilG	1547968..1549200 (+)	1233	WP_002216405.1	type 4 pilus assembly protein PilG	Machinery gene
DRA66_RS07745 (DRA66_09135)	pilD	1549274..1550134 (+)	861	WP_002241067.1	A24 family peptidase	Machinery gene
DRA66_RS07750 (DRA66_09140)	coaE	1550136..1550768 (+)	633	WP_002216402.1	dephospho-CoA kinase	-
DRA66_RS07755 (DRA66_09145)	-	1550761..1550970 (+)	210	WP_002216401.1	DNA gyrase inhibitor YacG	-
DRA66_RS07760 (DRA66_09150)	pilF	1551055..1552731 (-)	1677	WP_002221967.1	type IV-A pilus assembly ATPase PilB	Machinery gene
DRA66_RS07770 (DRA66_09170)	-	1553223..1553669 (-)	447	WP_002220271.1	DUF441 domain-containing protein	-
DRA66_RS07775 (DRA66_09175)	ispB	1553674..1554648 (-)	975	WP_002247958.1	octaprenyl diphosphate synthase	-

Sequence

Protein

• Length: 286 a.a.
• Molecular weight: 31331.48 Da
• Isoelectric Point: 9.1920

>NTDB_id=301731 DRA66_RS07745 WP_002241067.1 1549274..1550134(+) (pilD) [Neisseria meningitidis strain 12-208]
MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVFAKKHLNLPLTEKESRTFNLMKPDSCCPKCRVPIRAW
QNIPIVSYLLLRGKCASCQTKISIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGEIGMGNGDFKLIAALGAWLGISALPVLIFVSSLIGLV
AAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRAVNWWLTHPVL

Nucleotide

• Length: 861 bp

>NTDB_id=301731 DRA66_RS07745 WP_002241067.1 1549274..1550134(+) (pilD) [Neisseria meningitidis strain 12-208]
ATGTCTGATTTGTCTGTATTGTCGCCGTTTGCCGTGCCTTTGGCAGCGGTGTTCGGGCTGCTGGTCGGCAGTTTCTTAAA
CGTCGTCATTTACCGCGTGCCGGTCATGATGGAACGCGGCTGGACGGTATTTGCCAAAAAACATTTAAACCTGCCGCTGA
CCGAAAAGGAAAGCCGTACCTTCAACCTGATGAAACCGGATTCCTGCTGTCCCAAATGCCGCGTGCCGATACGCGCGTGG
CAGAACATCCCGATTGTCAGCTACCTGCTCCTGCGCGGCAAATGCGCTTCCTGCCAAACCAAAATCAGCATACGTTATCC
CTTAATCGAGCTGCTGACCGGCGTATTATTCGGGCTGGTCGCCTGGCAATACGGCTGGTCTTGGATTACATTGGGCGGTT
TGATACTGACCGCGTTTCTGATTTCCCTGACCTTTATCGATGCGGACACCCAATACCTGCCCGACTCGATGACACTGCCC
TTGATTTGGCTGGGGCTGATATTTAATTTGGACGGCGGCTTCGTGCCTTTGCAGTCTGCCGTTTTAGGTGCGGTTGCCGG
CTATGGTTCGATATGGCTTTTATGCGCGGTATATAAACTGATTAAAGGCGAAATCGGTATGGGCAACGGAGATTTCAAAC
TGATTGCCGCATTGGGCGCGTGGCTCGGCATATCCGCATTGCCCGTGCTGATTTTTGTTTCCTCGCTGATCGGTTTGGTC
GCGGCAATCGTTATGCGCGTCGCCAAGGGGCAGCATTTTGCCTTCGGCCCCGCACTGACAGTTTCGGGCTGGATAATTTT
TACGGCAAACGATTCCGTATGGCGGGCGGTCAACTGGTGGCTGACCCATCCGGTGCTGTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (134-242)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

3D structure

• AlphaFold DB: A1KVV2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Neisseria gonorrhoeae MS11	96.14	99.65	0.958
pilD	Vibrio cholerae strain A1552	44.444	100	0.448
pilD	Acinetobacter baumannii D1279779	46.97	92.308	0.434
pilD	Acinetobacter nosocomialis M2	46.97	92.308	0.434
pilD	Vibrio campbellii strain DS40M4	44.565	96.503	0.43