Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA   Type   Machinery gene
Locus tag   DRF63_RS04675 Genome accession   NZ_CP030753
Coordinates   1027546..1027992 (-) Length   148 a.a.
NCBI ID   WP_005597397.1    Uniprot ID   B0BPG5
Organism   Actinobacillus pleuropneumoniae strain S4074     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 1022546..1032992
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DRF63_RS04650 (DRF63_04645) yacG 1023453..1023641 (-) 189 WP_005597388.1 DNA gyrase inhibitor YacG -
  DRF63_RS04655 (DRF63_04650) coaE 1023623..1024261 (-) 639 WP_005597390.1 dephospho-CoA kinase -
  DRF63_RS04660 (DRF63_04655) - 1024283..1024921 (-) 639 WP_005597392.1 prepilin peptidase -
  DRF63_RS04665 (DRF63_04660) pilC 1024948..1026150 (-) 1203 WP_005597394.1 type II secretion system F family protein Machinery gene
  DRF63_RS04670 (DRF63_04665) pilB 1026134..1027528 (-) 1395 WP_005597396.1 GspE/PulE family protein Machinery gene
  DRF63_RS04675 (DRF63_04670) pilA 1027546..1027992 (-) 447 WP_005597397.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  DRF63_RS04680 (DRF63_04675) radA 1028174..1029553 (+) 1380 WP_005597398.1 DNA repair protein RadA -
  DRF63_RS04685 (DRF63_04680) - 1029597..1029830 (-) 234 WP_005597399.1 YgjV family protein -
  DRF63_RS04690 (DRF63_04685) folA 1029957..1030445 (-) 489 WP_005597400.1 type 3 dihydrofolate reductase -
  DRF63_RS04695 (DRF63_04690) proB 1030532..1031623 (+) 1092 WP_005597401.1 glutamate 5-kinase -
  DRF63_RS04700 (DRF63_04695) - 1031636..1032412 (+) 777 WP_005597402.1 TSUP family transporter -

Sequence


Protein


Download         Length: 148 a.a.        Molecular weight: 15980.38 Da        Isoelectric Point: 7.6257

>NTDB_id=301282 DRF63_RS04675 WP_005597397.1 1027546..1027992(-) (pilA) [Actinobacillus pleuropneumoniae strain S4074]
MQKLSLIRPLTNAFTLIELMIVIAIIAILATVAIPSYNSYTQKAALSELLAASASYKTDVEICIYNTGDSKNCSGGQNGV
RKMTELRQAKYLNAITVEGGTITVTGKGNLQEYGYTMTPIHNGSTISWETKCKGEDLSLFPANFCASN

Nucleotide


Download         Length: 447 bp        

>NTDB_id=301282 DRF63_RS04675 WP_005597397.1 1027546..1027992(-) (pilA) [Actinobacillus pleuropneumoniae strain S4074]
ATGCAAAAATTAAGTCTTATTCGACCGCTTACTAACGCGTTTACTTTAATTGAATTGATGATCGTGATTGCGATTATTGC
CATTTTAGCTACGGTTGCAATTCCGTCATATAACAGTTATACCCAAAAAGCGGCGCTTTCGGAGCTATTGGCGGCATCGG
CTTCTTATAAAACGGATGTCGAGATCTGCATATATAACACCGGAGATTCTAAAAACTGTAGCGGCGGTCAAAACGGTGTC
AGAAAAATGACGGAGCTTAGACAGGCTAAATATTTAAATGCCATTACGGTGGAAGGCGGAACGATTACAGTAACGGGGAA
AGGGAATCTACAGGAATACGGTTATACGATGACACCGATTCATAACGGTAGCACTATTTCTTGGGAAACGAAATGTAAAG
GGGAGGACTTAAGTTTATTTCCGGCAAATTTCTGCGCATCAAATTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB B0BPG5

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

68.919

100

0.689

  pilA Haemophilus influenzae 86-028NP

55.102

99.324

0.547

  pilA Haemophilus influenzae Rd KW20

53.061

99.324

0.527


Multiple sequence alignment