Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   DQ228_RS01775 Genome accession   NZ_CP030250
Coordinates   328149..329468 (+) Length   439 a.a.
NCBI ID   WP_232979810.1    Uniprot ID   -
Organism   Streptococcus thermophilus strain CS20     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 323149..334468
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQ228_RS01750 - 324003..324767 (+) 765 WP_002949740.1 ABC transporter ATP-binding protein -
  DQ228_RS01755 - 324767..325477 (+) 711 WP_011680767.1 ABC transporter ATP-binding protein -
  DQ228_RS01760 - 325613..326273 (+) 661 Protein_322 CBS and ACT domain-containing protein -
  DQ228_RS01765 cysK 326440..327366 (-) 927 WP_011680768.1 cysteine synthase A -
  DQ228_RS01770 - 327468..328094 (-) 627 WP_011680769.1 YigZ family protein -
  DQ228_RS01775 comFA/cflA 328149..329468 (+) 1320 WP_232979810.1 DEAD/DEAH box helicase Machinery gene
  DQ228_RS01780 - 329449..330111 (+) 663 WP_011680771.1 ComF family protein -
  DQ228_RS01785 hpf 330190..330738 (+) 549 WP_011680772.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  DQ228_RS01800 - 331464..332396 (+) 933 WP_011680773.1 manganese-dependent inorganic pyrophosphatase -
  DQ228_RS01805 - 332450..333109 (+) 660 WP_011225520.1 DUF1803 domain-containing protein -
  DQ228_RS01810 - 333139..333672 (-) 534 WP_002949762.1 DUF402 domain-containing protein -

Sequence

Protein


Download         Length: 439 a.a.        Molecular weight: 50627.82 Da        Isoelectric Point: 9.7105

>NTDB_id=300413 DQ228_RS01775 WP_232979810.1 328149..329468(+) (comFA/cflA) [Streptococcus thermophilus strain CS20]
MIPKEYYGRLFTKEQLPVDYLSEAVKLESMIKVDKKLRCKRCYSRIEEDWQLPNGQYYCRACIVFGRNQEGKELYYFPSE
KSEVDFPVLKWSGKLTPYQNEVSEKLLKTYKNQKHSLVHAVIGAGKTEMIYNIVAYVLENKNRVVIASPRVDVCRELFLR
MQKDFTCSISLLHADSEPYDGSPLVIATTHQLLKFYHSFDLIIVDEVDAFPFVGNVMLNHAVKQAKTETGRYIYLTATST
LALEEQVRLGAIEKHHLASRFHGNPLVLPRFFWQGRLQKSLTSEKLPRPLIHQIKKQRKSNFPLLIFFPNIALGEKFSIT
LKKYLPTENIAFVSSKSEERSTIVEKFRKKELTILVTTTILERGVTFPQVDVFVCMANHHLYTSSSLIQIGGRVGRSPER
PTGKLYFFHEGLSKSMLQCRKEINAMNKKGGFENEVSTM

Nucleotide


Download         Length: 1320 bp        

>NTDB_id=300413 DQ228_RS01775 WP_232979810.1 328149..329468(+) (comFA/cflA) [Streptococcus thermophilus strain CS20]
ATGATACCTAAAGAATATTATGGACGACTATTTACGAAAGAACAGTTACCAGTGGATTATCTCTCAGAGGCTGTAAAATT
AGAAAGTATGATAAAGGTTGATAAAAAACTTAGATGTAAAAGATGTTATAGTCGAATAGAGGAAGATTGGCAATTACCGA
ATGGTCAGTATTATTGTAGAGCGTGTATTGTCTTTGGTCGAAACCAAGAAGGAAAAGAACTCTATTACTTTCCCTCAGAA
AAATCAGAAGTAGATTTTCCTGTCTTGAAATGGTCAGGAAAACTGACTCCTTATCAAAATGAGGTCTCGGAAAAGCTTTT
AAAGACTTATAAAAATCAAAAACACAGTCTTGTTCATGCAGTGATTGGTGCTGGCAAGACAGAGATGATTTATAATATTG
TTGCCTATGTTCTTGAAAATAAAAATCGTGTCGTCATCGCAAGTCCCCGAGTTGATGTTTGTCGAGAATTGTTTCTACGC
ATGCAGAAAGATTTTACTTGTAGTATTTCTCTGCTTCATGCTGATAGTGAACCATATGATGGTAGTCCGCTCGTTATAGC
TACCACTCATCAATTACTAAAATTTTATCATAGCTTTGACTTGATTATTGTTGACGAAGTTGATGCCTTTCCATTTGTAG
GGAATGTCATGTTAAATCATGCTGTTAAACAGGCAAAGACGGAAACAGGCCGGTATATTTACTTGACAGCAACTTCTACA
TTAGCTTTAGAAGAGCAAGTGCGCCTTGGAGCTATAGAAAAGCATCACCTTGCTAGTCGTTTCCACGGAAATCCTCTAGT
CCTTCCTCGTTTCTTTTGGCAAGGAAGGTTACAAAAGTCGTTGACGAGCGAGAAGCTTCCAAGGCCTCTAATTCACCAGA
TTAAGAAGCAGCGTAAATCAAATTTTCCTCTATTAATCTTTTTCCCCAATATAGCATTAGGTGAAAAGTTTAGTATTACC
CTAAAAAAATATCTCCCTACTGAAAACATAGCCTTTGTTTCATCAAAAAGCGAGGAGCGTTCAACCATCGTAGAGAAATT
CCGAAAAAAAGAATTGACAATCTTAGTGACGACAACTATTCTCGAACGTGGTGTTACCTTTCCACAAGTAGATGTTTTTG
TTTGTATGGCAAATCATCACTTATATACTAGTTCGAGTCTTATTCAGATTGGTGGTAGGGTGGGGCGTTCGCCCGAGAGA
CCTACAGGGAAACTCTATTTCTTTCATGAAGGATTATCTAAATCAATGTTGCAATGTCGGAAAGAAATAAATGCAATGAA
TAAAAAAGGAGGGTTTGAAAATGAAGTGTCTACTATGTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(290-396)

Type III restriction enzyme, res subunit (ResIII)

(94-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae TIGR4

55.22

98.178

0.542
  comFA/cflA Streptococcus pneumoniae Rx1

55.22

98.178

0.542
  comFA/cflA Streptococcus pneumoniae D39

55.22

98.178

0.542
  comFA/cflA Streptococcus pneumoniae R6

55.22

98.178

0.542
  comFA/cflA Streptococcus mitis NCTC 12261

54.884

97.95

0.538
  comFA/cflA Streptococcus mitis SK321

53.953

97.95

0.528
  comFA Lactococcus lactis subsp. cremoris KW2

45.476

95.672

0.435
  comFA Latilactobacillus sakei subsp. sakei 23K

36.782

99.089

0.364

Multiple sequence alignment