Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   DOU34_RS07065 Genome accession   NZ_CP030097
Coordinates   1382819..1383718 (+) Length   299 a.a.
NCBI ID   WP_020955817.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain SH-B74     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1377819..1388718
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DOU34_RS07045 - 1378509..1380212 (+) 1704 WP_061046790.1 hypothetical protein -
  DOU34_RS07050 - 1380209..1380490 (+) 282 WP_007409769.1 FlhB-like flagellar biosynthesis protein -
  DOU34_RS07055 sucC 1380665..1381822 (+) 1158 WP_003154283.1 ADP-forming succinate--CoA ligase subunit beta -
  DOU34_RS07060 sucD 1381851..1382753 (+) 903 WP_003154281.1 succinate--CoA ligase subunit alpha -
  DOU34_RS07065 dprA 1382819..1383718 (+) 900 WP_020955817.1 DNA-processing protein DprA Machinery gene
  DOU34_RS07070 topA 1383900..1385975 (+) 2076 WP_007409771.1 type I DNA topoisomerase -
  DOU34_RS07075 trmFO 1386040..1387347 (+) 1308 WP_012117550.1 FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO -
  DOU34_RS07080 xerC 1387417..1388334 (+) 918 WP_007409774.1 tyrosine recombinase XerC -

Sequence

Protein


Download         Length: 299 a.a.        Molecular weight: 32836.95 Da        Isoelectric Point: 7.5071

>NTDB_id=299110 DOU34_RS07065 WP_020955817.1 1382819..1383718(+) (dprA) [Bacillus amyloliquefaciens strain SH-B74]
MDQASRCLMVCSINQIISPSLLLKWWKADHSLSFLPDPHPLTVLSEGKTAPEAIFQEIERKDPELDEVLSDYRREGITVI
PISSSRYPTWLKAIYDPPAVLYAKGNTLLLEKGRKIGIVGTRKPTEDGIKAVGHLSAELSKKGWVIVSGLASGIDGLSHK
ASIRAKGLTIGVIAGGFHHIYPRENLLLAEYMAEHHLLLSEHPPETKPKKWHFPMRNRIISGLSEGIVVVQGKEKSGSLI
TAYQALDQGREVFAVPGSIFNPYSGGPIKLIQEGAKAVLCAEDIDGELTARCVQYTEPF

Nucleotide


Download         Length: 900 bp        

>NTDB_id=299110 DOU34_RS07065 WP_020955817.1 1382819..1383718(+) (dprA) [Bacillus amyloliquefaciens strain SH-B74]
TTGGATCAAGCATCGCGCTGTTTAATGGTCTGCAGTATTAATCAAATCATTTCCCCGTCTCTTCTATTAAAATGGTGGAA
AGCTGATCATTCTCTGTCTTTTTTACCGGATCCGCATCCATTAACTGTTTTATCAGAAGGGAAAACAGCCCCGGAAGCAA
TTTTTCAGGAAATAGAGCGCAAGGATCCGGAACTTGATGAAGTTCTGTCCGATTACCGCCGCGAAGGCATTACTGTCATT
CCGATTTCATCAAGCCGCTATCCAACATGGCTTAAAGCGATTTATGATCCGCCGGCTGTCTTGTATGCAAAAGGGAACAC
GCTGCTTCTTGAAAAAGGCAGAAAAATCGGGATTGTAGGAACGCGGAAACCGACGGAAGACGGAATAAAAGCGGTTGGGC
ATCTTTCCGCCGAACTCTCAAAAAAAGGCTGGGTCATTGTAAGCGGGCTTGCATCCGGTATAGACGGATTGTCTCATAAG
GCGAGCATCAGGGCAAAAGGGCTTACGATCGGCGTGATAGCCGGCGGATTCCATCACATCTATCCCCGGGAAAATCTCCT
GTTAGCAGAATACATGGCTGAACACCATCTCCTACTCTCAGAACATCCTCCTGAAACAAAGCCGAAAAAATGGCACTTTC
CGATGAGAAACCGCATAATCAGCGGATTAAGTGAAGGAATTGTGGTCGTGCAGGGAAAAGAAAAAAGCGGTTCATTAATC
ACAGCTTACCAGGCTCTCGATCAAGGCAGAGAGGTATTTGCCGTTCCGGGTTCCATATTTAATCCATATTCCGGAGGACC
TATAAAACTCATTCAAGAAGGGGCGAAAGCTGTATTATGCGCAGAGGATATTGACGGAGAGCTGACCGCCCGATGCGTTC
AGTATACGGAACCCTTTTGA

Domains

Predicted by InterProScan.

DNA recombination-mediator protein A (DNA_processg_A)

(73-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Bacillus subtilis subsp. subtilis str. 168

70.903

100

0.709
  dprA Lactococcus lactis subsp. cremoris KW2

42.045

88.294

0.371

Multiple sequence alignment