Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilJ   Type   Machinery gene
Locus tag   MAE_RS14450 Genome accession   NC_010296
Coordinates   3045517..3048195 (-) Length   892 a.a.
NCBI ID   WP_012266253.1    Uniprot ID   B0JM81
Organism   Microcystis aeruginosa NIES-843     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3040517..3053195
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MAE_RS14440 (MAE_33410) - 3042448..3043785 (-) 1338 WP_012266250.1 AAA-like domain-containing protein -
  MAE_RS14445 (MAE_33430) - 3044232..3045443 (-) 1212 WP_012266252.1 glycosyltransferase -
  MAE_RS14450 (MAE_33440) pilJ 3045517..3048195 (-) 2679 WP_012266253.1 methyl-accepting chemotaxis protein Machinery gene
  MAE_RS14455 (MAE_33450) - 3048285..3048815 (-) 531 WP_002798117.1 chemotaxis protein CheW -
  MAE_RS14460 (MAE_33460) pilH 3048907..3049272 (-) 366 WP_002798118.1 response regulator transcription factor Machinery gene
  MAE_RS14465 (MAE_33490) - 3050882..3052213 (+) 1332 WP_012266256.1 RNA-guided endonuclease InsQ/TnpB family protein -

Sequence

Protein


Download         Length: 892 a.a.        Molecular weight: 96895.72 Da        Isoelectric Point: 4.4333

>NTDB_id=29873 MAE_RS14450 WP_012266253.1 3045517..3048195(-) (pilJ) [Microcystis aeruginosa NIES-843]
MPSGTEYAKLYRKANTAYCQGNLEDAAVIVKEMISKYPEDANVMLLQGHIHLGLQQYRLAEERYEKVLQLAKNSPTSSDL
VDYAQRGLDQIRQLRFEAEEGDFTIAATESIAYSEAAFDPSQSFNRSRSWKSQGAAPDGQKKGLDWNSSLFNEEDPSEPT
VSKVHSYDDDFEESEETAFSSLTLPGPGSHGSQWSSDSAEGEAPFPFLEAAEIDSLEEGWDGELGSTGEATFMVSSALGG
NPLTGRDQELSYRDTLEESTFAIDLESTHPQGAKTGPNQGRVTQQRDEEDETGDFGQPELNDEDLDGFSPLALTDTVQGL
GDWELFTPSGGEALSGGFLPEPSAHLNASSGVGEPSFNPSRLKTPNDKRDSHLTHASELLLKPVVEVNPGKLGFFVNASL
GKKQLILAALAGITPVIIIFAVSTTSWLSTLLAAKPAPNSQAKTEKVAPKPAPKPPASASLSPFSQPVLTTMLLTGLGTL
ALTWLGLQLLISQIKRSLNDLQSQFDHLSEGNFNAKATIYSEDEFGQLANRFNQMARVVVTTTTEAQRRAAETEREREDL
QRQVIRLLDDVEGAARGDLTVEAEVSADVLGAVADAFNLTIQNLREIVRQVKKAAKQVNQGSTNSESFARNQSSDALRMA
EELAVTLNSVQMMTDSIQRVAENAREAEEVARTSSITALKGGDSVERTVAGILQIRETVSETARKVKRLAEASQEISKIV
AVVSQIASRTNLLALNASIQAARAGEAGRGFAVVADEVRQLADRAAKSLKEIEQIVLHIQSETGSVMTAMEEGIQQVIDV
TERSEQAKKSLEDIIQVSNRIDTLVRSITADTVKQRENSLNVAQVMQSVELTAQETSQESQRVAGSLQKLVSISRELLSS
VERFKVDSEDDR

Nucleotide


Download         Length: 2679 bp        

>NTDB_id=29873 MAE_RS14450 WP_012266253.1 3045517..3048195(-) (pilJ) [Microcystis aeruginosa NIES-843]
ATGCCGTCAGGGACAGAATACGCAAAACTTTATAGAAAGGCTAACACCGCCTATTGCCAGGGCAACCTTGAGGATGCCGC
CGTCATTGTCAAAGAGATGATCAGCAAGTATCCCGAGGATGCCAATGTCATGCTTTTACAGGGTCACATTCATCTAGGAC
TACAACAATACAGATTGGCGGAGGAACGTTACGAAAAAGTGCTGCAATTGGCTAAAAATTCTCCCACTAGCTCCGATCTA
GTCGATTATGCCCAGCGTGGTCTCGATCAGATTCGGCAATTGCGTTTTGAAGCCGAGGAGGGAGATTTTACGATCGCTGC
TACGGAAAGCATTGCCTACAGCGAGGCAGCTTTCGATCCAAGTCAAAGTTTTAACCGTTCTCGCTCCTGGAAGTCGCAAG
GGGCTGCCCCCGATGGACAAAAAAAAGGGCTGGATTGGAATAGTAGTCTTTTCAATGAGGAGGATCCCAGCGAGCCTACG
GTAAGTAAAGTCCATTCCTATGACGATGATTTTGAGGAATCGGAGGAAACCGCCTTTAGTTCCCTTACCCTGCCGGGCCC
TGGCTCTCACGGTTCCCAGTGGTCTAGCGATTCGGCGGAGGGGGAAGCCCCTTTCCCCTTTTTAGAAGCGGCGGAGATTG
ACTCGCTGGAGGAAGGGTGGGACGGTGAGTTAGGGTCAACGGGAGAAGCCACTTTTATGGTTTCCTCTGCTCTGGGGGGA
AATCCCCTCACCGGTAGAGATCAGGAGTTATCCTATAGGGATACCCTAGAAGAAAGCACTTTTGCCATTGACCTGGAATC
GACCCACCCACAAGGGGCAAAAACTGGCCCTAATCAGGGCAGAGTCACTCAACAAAGAGACGAGGAAGACGAAACCGGAG
ATTTTGGGCAACCAGAACTCAATGATGAGGATTTAGACGGTTTTTCCCCACTGGCTCTGACGGATACGGTTCAAGGACTG
GGGGACTGGGAGCTATTTACCCCTTCGGGGGGGGAAGCTTTAAGTGGTGGTTTTCTCCCCGAACCCAGTGCGCACCTTAA
TGCCAGTTCTGGTGTGGGAGAGCCTAGTTTTAATCCTTCTCGTCTAAAAACCCCCAATGACAAAAGAGATAGTCATCTTA
CCCATGCCTCCGAACTCTTGCTCAAGCCAGTGGTGGAAGTCAATCCGGGCAAATTAGGCTTTTTTGTCAACGCTTCTTTA
GGCAAAAAACAACTAATCCTAGCGGCCTTAGCGGGTATTACCCCTGTGATCATAATTTTTGCCGTTAGTACCACCTCTTG
GCTTTCTACGCTCTTGGCTGCCAAACCAGCCCCGAATAGCCAGGCAAAAACCGAAAAGGTTGCCCCCAAACCGGCCCCGA
AACCGCCAGCCTCAGCCAGTCTTTCCCCCTTCAGTCAACCGGTACTAACCACGATGTTATTAACCGGACTGGGAACCCTA
GCCCTGACTTGGCTAGGTTTACAGTTGCTAATTAGTCAGATTAAGCGCAGTCTCAACGATCTGCAAAGCCAATTCGATCA
TCTCTCGGAAGGTAATTTTAACGCTAAAGCGACGATCTACTCGGAAGATGAATTCGGTCAACTGGCCAATCGTTTCAATC
AGATGGCTCGGGTGGTGGTAACTACCACCACGGAAGCCCAACGCCGCGCCGCCGAAACGGAACGGGAACGGGAAGACTTA
CAACGGCAAGTGATTCGACTTCTCGATGATGTGGAAGGAGCGGCTCGGGGAGATCTGACCGTGGAAGCGGAGGTAAGCGC
CGATGTTTTAGGGGCGGTGGCCGATGCTTTTAATTTGACTATCCAAAACCTGCGCGAAATTGTCCGACAGGTGAAAAAAG
CGGCCAAACAGGTGAATCAAGGCAGTACCAATAGTGAATCTTTCGCCCGCAATCAGTCTAGCGATGCCCTGCGGATGGCG
GAGGAATTGGCCGTCACCCTTAATTCGGTACAGATGATGACCGACTCAATTCAACGGGTGGCAGAAAACGCCAGAGAAGC
CGAAGAAGTCGCCCGCACTTCCTCGATTACCGCCCTGAAGGGGGGCGATTCCGTGGAAAGAACCGTGGCGGGAATTCTAC
AAATCCGCGAGACCGTATCAGAAACGGCGCGAAAAGTCAAGCGTTTGGCGGAAGCTTCCCAGGAAATCTCGAAAATTGTC
GCGGTAGTCTCTCAGATTGCCTCGCGGACCAATTTATTGGCTCTTAATGCCTCGATTCAAGCGGCGCGAGCAGGAGAGGC
GGGCCGCGGTTTTGCGGTTGTGGCCGATGAGGTGCGTCAGTTGGCCGATAGAGCCGCTAAATCGCTTAAGGAAATTGAAC
AGATCGTTTTACATATCCAATCGGAAACGGGTTCGGTAATGACGGCGATGGAGGAAGGGATTCAACAGGTTATCGATGTC
ACCGAGCGCTCGGAACAGGCGAAGAAGTCCCTAGAAGATATTATCCAAGTTTCTAATCGCATCGATACGCTGGTGCGATC
GATTACCGCCGATACGGTCAAACAACGGGAAAATTCCCTCAATGTCGCCCAAGTTATGCAGTCGGTGGAGTTAACCGCTC
AGGAAACTTCCCAAGAATCCCAACGGGTGGCGGGTTCTCTGCAAAAATTGGTGAGTATCTCCCGGGAGCTGCTTTCTTCG
GTAGAACGGTTTAAGGTAGACTCAGAGGATGATCGATAA

Domains

Predicted by InterproScan.

HAMP domain (HAMP)

(489-537)

Methyl-accepting chemotaxis protein (MCP) signalling domain (MCPsignal)

(669-849)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B0JM81

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilJ Synechocystis sp. PCC 6803

49.892

100

0.519

Multiple sequence alignment