Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MAE_RS09675 Genome accession   NC_010296
Coordinates   1985921..1987447 (-) Length   508 a.a.
NCBI ID   WP_002796849.1    Uniprot ID   I4HQJ6
Organism   Microcystis aeruginosa NIES-843     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1983372..2016293 1985921..1987447 within 0


Gene organization within MGE regions


Location: 1983372..2016293
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MAE_RS09645 (MAE_22100) - 1983372..1983830 (-) 459 WP_002799586.1 DUF29 domain-containing protein -
  MAE_RS09650 (MAE_22110) - 1983843..1984298 (-) 456 WP_012265410.1 DUF29 domain-containing protein -
  MAE_RS09655 (MAE_22120) - 1984311..1984766 (-) 456 WP_012265411.1 DUF29 domain-containing protein -
  MAE_RS09660 (MAE_22130) - 1984778..1985233 (-) 456 WP_002796852.1 DUF29 domain-containing protein -
  MAE_RS09665 (MAE_22140) - 1985246..1985701 (-) 456 WP_002796850.1 DUF29 domain-containing protein -
  MAE_RS09670 petL 1985774..1985875 (-) 102 WP_002752397.1 cytochrome b6-f complex subunit PetL -
  MAE_RS09675 (MAE_22150) radA 1985921..1987447 (-) 1527 WP_002796849.1 DNA repair protein RadA Machinery gene
  MAE_RS09680 (MAE_22160) - 1987681..1988409 (+) 729 WP_041804658.1 response regulator transcription factor RpaB -
  MAE_RS09685 (MAE_22170) - 1988419..1989060 (+) 642 WP_002796847.1 cofactor assembly of complex C subunit B -
  MAE_RS09690 (MAE_22180) - 1989151..1990308 (-) 1158 WP_012265412.1 IS630-like element ISMae23 family transposase -
  MAE_RS09695 (MAE_22200) - 1990752..1991966 (+) 1215 WP_012265414.1 ISL3-like element ISMae36 family transposase -
  MAE_RS09700 (MAE_22210) - 1992571..1993428 (-) 858 Protein_2028 IS5 family transposase -
  MAE_RS09710 (MAE_22230) - 1993687..1993941 (+) 255 Protein_2029 helix-turn-helix domain-containing protein -
  MAE_RS09715 (MAE_22240) - 1994132..1994884 (+) 753 Protein_2030 IS630-like element ISMae24 family transposase -
  MAE_RS34500 - 1994910..1995028 (-) 119 Protein_2031 ISNCY family transposase -
  MAE_RS09720 (MAE_22250) - 1995138..1996124 (+) 987 WP_012265332.1 IS30-like element ISMae39 family transposase -
  MAE_RS35680 (MAE_22260) - 1996336..1996716 (-) 381 WP_012265419.1 ISAzo13-like element transposase-related protein -
  MAE_RS09730 (MAE_22270) - 1996782..1997543 (+) 762 Protein_2034 IS630 family transposase -
  MAE_RS29155 - 1997635..1998696 (+) 1062 Protein_2035 ISKra4 family transposase -
  MAE_RS09740 (MAE_22290) - 1998929..2000197 (+) 1269 WP_012264345.1 IS701-like element ISMae34 family transposase -
  MAE_RS09745 (MAE_22300) - 2000311..2000601 (+) 291 Protein_2037 transposase -
  MAE_RS09750 - 2000588..2000872 (-) 285 WP_041803987.1 ISAzo13-like element transposase-related protein -
  MAE_RS09755 (MAE_22320) - 2001074..2002288 (+) 1215 Protein_2039 ISL3-like element ISMae36 family transposase -
  MAE_RS29160 - 2002222..2002632 (-) 411 WP_174718080.1 hypothetical protein -
  MAE_RS09760 - 2002709..2003364 (+) 656 Protein_2041 adenosine kinase -
  MAE_RS09765 (MAE_22350) - 2003453..2004496 (+) 1044 WP_012265427.1 IS630 family transposase -
  MAE_RS31945 - 2005030..2005134 (+) 105 Protein_2043 PfkB family carbohydrate kinase -
  MAE_RS09775 (MAE_22370) - 2005197..2006267 (+) 1071 WP_012265278.1 IS630-like element ISMae27 family transposase -
  MAE_RS31950 - 2006274..2006411 (+) 138 Protein_2045 adenosine kinase -
  MAE_RS09780 (MAE_22380) - 2006876..2007130 (-) 255 WP_012265429.1 hypothetical protein -
  MAE_RS09785 - 2007251..2007523 (-) 273 WP_002757090.1 hypothetical protein -
  MAE_RS09790 (MAE_22400) - 2007755..2008102 (+) 348 Protein_2048 transposase -
  MAE_RS09795 (MAE_22410) - 2008137..2008955 (-) 819 WP_012265431.1 class I SAM-dependent methyltransferase family protein -
  MAE_RS29185 (MAE_22440) - 2010326..2010589 (-) 264 WP_012265432.1 class I SAM-dependent methyltransferase family protein -
  MAE_RS09810 (MAE_22450) - 2010631..2011272 (-) 642 WP_012265433.1 lysophospholipid acyltransferase family protein -
  MAE_RS09815 (MAE_22460) - 2011269..2012225 (-) 957 WP_002768798.1 phosphatidate cytidylyltransferase -
  MAE_RS09820 (MAE_22470) - 2012200..2012832 (-) 633 WP_002768800.1 CDP-alcohol phosphatidyltransferase family protein -
  MAE_RS09825 (MAE_22480) - 2013651..2014001 (-) 351 WP_012265434.1 hypothetical protein -
  MAE_RS09830 (MAE_22490) - 2013998..2014372 (-) 375 WP_002784772.1 DUF433 domain-containing protein -
  MAE_RS09835 (MAE_22520) - 2014879..2015343 (-) 465 WP_002796831.1 DUF29 domain-containing protein -
  MAE_RS09840 (MAE_22530) - 2015360..2015818 (-) 459 WP_012265437.1 DUF29 domain-containing protein -
  MAE_RS09845 (MAE_22540) - 2015835..2016293 (-) 459 WP_002758350.1 DUF29 domain-containing protein -

Sequence

Protein


Download         Length: 508 a.a.        Molecular weight: 54878.38 Da        Isoelectric Point: 4.9634

>NTDB_id=29870 MAE_RS09675 WP_002796849.1 1985921..1987447(-) (radA) [Microcystis aeruginosa NIES-843]
MAKSRTIYICSACGAESPQWLGKCPSCDTYGTLEEQVVAGGNNPAVNRPGWQNSRPNNGKGQRNPQPRISVRFSEITQYE
QERFPSGYGEFDRVLGGGIVPGSLVLIGGDPGIGKSTLLLQVTNQLAQRLPRILYVSAEESGQQIKLRAARLGVGVVAVE
SENNGNGKEKKASESTAELDNHLYVLPETDLEEILRELESLRPQVAVIDSIQTLYFGALTSAPGSVAQVRECTSALMQVA
KRENITLLIVGHVTKEGAIAGPRVLEHLVDTVLYFEGDRYASHRLLRSVKNRFGATHEIGIFEMVDHGLVEVENPSELFL
GNRDEFSPGTATVVACEGTRPIVVELQALVSPTSYASPRRSTTGIEYNRLQQILAVLEKRVGIPLSKLDAYVASAGGLGV
EEPAADLGVAIAVVASFRDRVVDPRTVLIGEVGLGGQVRLVSQMELRLKEAAKLGFKRAIVPRGQVYPEDVGLEIVPVGK
VIEAIVAAIPPQQRFGEDIEDEEGEGQE

Nucleotide


Download         Length: 1527 bp        

>NTDB_id=29870 MAE_RS09675 WP_002796849.1 1985921..1987447(-) (radA) [Microcystis aeruginosa NIES-843]
ATGGCGAAATCGCGAACAATATATATCTGTAGTGCTTGTGGGGCGGAATCTCCCCAATGGTTGGGAAAATGCCCCAGTTG
TGACACCTACGGGACGCTAGAAGAACAAGTGGTAGCTGGTGGCAATAATCCGGCGGTAAATCGCCCAGGATGGCAAAATA
GTCGCCCAAATAACGGCAAAGGGCAACGCAACCCCCAGCCGCGCATCTCGGTTCGCTTCTCGGAAATTACCCAGTATGAA
CAGGAACGTTTTCCCTCCGGTTACGGGGAATTTGATCGCGTTCTCGGTGGTGGCATCGTCCCCGGTTCTCTTGTACTAAT
CGGCGGCGATCCGGGCATCGGCAAATCGACGCTTTTACTGCAAGTCACCAACCAACTCGCCCAAAGATTACCGCGCATCC
TCTACGTTTCCGCCGAAGAATCGGGACAACAGATTAAACTGCGGGCTGCCCGTTTGGGGGTGGGAGTGGTTGCCGTGGAG
TCGGAAAATAACGGCAATGGCAAGGAGAAAAAAGCCTCTGAATCAACCGCAGAACTCGATAATCATCTCTACGTTCTGCC
AGAAACAGATTTAGAGGAAATTTTGCGAGAATTAGAATCTCTACGCCCACAGGTGGCGGTAATTGACAGTATTCAAACTC
TTTATTTTGGGGCGTTAACTTCCGCACCGGGATCTGTCGCCCAAGTTCGCGAATGTACCTCCGCCTTGATGCAGGTGGCA
AAACGGGAGAATATTACTTTATTGATTGTCGGTCATGTTACTAAAGAAGGGGCGATCGCCGGTCCGCGAGTTTTGGAACA
TTTGGTCGATACGGTGTTATATTTTGAAGGCGATCGCTATGCCTCCCATCGTCTCTTAAGATCCGTGAAAAACCGTTTTG
GGGCAACCCATGAGATCGGTATTTTCGAGATGGTGGATCACGGTTTGGTGGAAGTGGAAAACCCCTCAGAATTGTTTTTA
GGCAATCGCGACGAATTTTCCCCTGGTACGGCGACGGTGGTAGCCTGTGAAGGTACACGCCCGATTGTGGTGGAGTTACA
GGCATTAGTTAGCCCCACCAGCTACGCTTCCCCGCGAAGATCGACCACGGGCATAGAATACAACCGATTACAGCAAATTC
TCGCCGTTTTAGAAAAGCGCGTCGGCATTCCTTTATCGAAATTAGATGCCTATGTTGCCTCCGCAGGAGGGTTGGGAGTG
GAAGAACCGGCGGCGGATCTGGGAGTTGCGATCGCTGTTGTCGCCAGTTTTCGCGATCGAGTCGTGGATCCGCGCACAGT
CTTAATCGGGGAAGTGGGATTAGGCGGACAAGTGCGCCTAGTGTCGCAAATGGAACTAAGATTAAAAGAAGCGGCTAAAT
TGGGCTTTAAACGGGCGATTGTCCCTAGAGGTCAAGTTTATCCCGAAGATGTGGGCTTAGAAATTGTTCCAGTCGGCAAG
GTAATCGAGGCAATTGTCGCCGCAATCCCCCCCCAGCAGCGCTTTGGGGAAGATATAGAAGACGAGGAAGGGGAAGGGCA
AGAATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(401-463)

KaiC (ATPase)

(85-296)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I4HQJ6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.924

95.866

0.488
  radA Streptococcus mitis SK321

46.555

94.291

0.439
  radA Streptococcus pneumoniae D39

47.21

91.732

0.433
  radA Streptococcus pneumoniae TIGR4

47.21

91.732

0.433
  radA Streptococcus mitis NCTC 12261

47.21

91.732

0.433
  radA Streptococcus pneumoniae R6

47.21

91.732

0.433
  radA Streptococcus pneumoniae Rx1

47.21

91.732

0.433

Multiple sequence alignment