Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   MAE_RS01865 Genome accession   NC_010296
Coordinates   369190..371205 (+) Length   671 a.a.
NCBI ID   WP_012264075.1    Uniprot ID   B0JN67
Organism   Microcystis aeruginosa NIES-843     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 364190..376205
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MAE_RS01845 (MAE_04040) rnpA 364540..364902 (-) 363 WP_002797764.1 ribonuclease P protein component -
  MAE_RS28205 (MAE_04050) rpmH 364929..365066 (-) 138 WP_002739276.1 50S ribosomal protein L34 -
  MAE_RS01850 (MAE_04060) - 365157..367121 (+) 1965 WP_002797765.1 amylo-alpha-1,6-glucosidase -
  MAE_RS01855 (MAE_04070) - 367469..368137 (+) 669 WP_002797767.1 HAD-IA family hydrolase -
  MAE_RS01860 (MAE_04080) grpE 368227..368949 (-) 723 WP_012264074.1 nucleotide exchange factor GrpE -
  MAE_RS01865 (MAE_04090) pilB/pilB1 369190..371205 (+) 2016 WP_012264075.1 GspE/PulE family protein Machinery gene
  MAE_RS01870 (MAE_04100) pilT 371351..372451 (+) 1101 WP_002797770.1 type IV pilus twitching motility protein PilT Machinery gene
  MAE_RS01875 (MAE_04110) - 372618..373832 (+) 1215 WP_012264076.1 type II secretion system F family protein -
  MAE_RS31370 (MAE_04120) - 373928..374137 (+) 210 WP_012264077.1 hypothetical protein -
  MAE_RS01885 (MAE_04130) - 374219..374683 (+) 465 Protein_383 hypothetical protein -

Sequence

Protein


Download         Length: 671 a.a.        Molecular weight: 74855.92 Da        Isoelectric Point: 6.4257

>NTDB_id=29864 MAE_RS01865 WP_012264075.1 369190..371205(+) (pilB/pilB1) [Microcystis aeruginosa NIES-843]
MTFSSKKTRAVALRNYFSPFGNKLVQSGYIGADQMQRALVETRKSGRSLVEILQQLTGRPLPPDLQRQYKKNQLFELKIL
YGVESIDPELTDVDGLEIARLIDSLIPIDLCRRYRLIPLSRIEGEPAAILIAMVDPDNLEATDDINRILRPRELGLQRLV
ITSEDYERLLEKFHSVQSELEQEKARLQKEKELAKLSDLTGIVSNIEVASGAANDEVADDLGEGEANQAPVINLVNKILA
KALQEGTSDIHIEPQEEFLKIRFRKDGVLHQAFEPLPKKITPAVTARFKIMADLDIAERRLPQDGKIRRLYQGRTVDFRV
NTLPSRYGEKVCLRILDNSATQLGLDKLITDQKTLQIVRDLASHPFGLLLVTGPTGSGKSTTLYSVLAERNHPGVNISTA
EDPIEYSLPGITQVQVIREKGMDFASILRAFMRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAAGAIARLD
KMGVEPFMISGSLLGVLAQRLVRRVCSQCRVAYNPSQGELARFGLAAANEREVTFYKANTLTLEEIQQARKQGNLCLKCS
GSGYKGRIGVYEVMQNSERLQQLIYQGATTDRIKEAAVDEGMVTLLAYSLNLVREGYTTLEEVERVTFTDSGLEAELKAK
RKSGLICRGCRAELQPEWLDCPYCMTPRFSD

Nucleotide


Download         Length: 2016 bp        

>NTDB_id=29864 MAE_RS01865 WP_012264075.1 369190..371205(+) (pilB/pilB1) [Microcystis aeruginosa NIES-843]
ATGACATTTTCTTCCAAGAAAACCCGTGCCGTTGCCCTGCGCAATTACTTTTCGCCCTTTGGTAACAAGTTGGTGCAGTC
GGGCTATATCGGTGCCGACCAGATGCAACGGGCCTTAGTGGAAACACGGAAATCGGGACGATCGCTAGTAGAAATATTAC
AACAACTAACCGGGCGCCCTTTGCCCCCGGATTTACAGCGACAGTATAAGAAAAACCAGTTATTTGAACTAAAAATTCTC
TACGGAGTCGAATCGATCGACCCCGAATTAACCGATGTGGATGGACTGGAAATAGCCCGATTGATCGATTCTTTGATTCC
GATCGATCTTTGTCGTCGCTATCGTCTCATCCCCCTGAGTCGGATCGAAGGAGAGCCGGCTGCGATTTTGATAGCCATGG
TCGATCCCGACAACCTCGAGGCTACCGACGATATCAATCGTATCCTCCGCCCCAGGGAGTTAGGTTTACAGCGTTTGGTG
ATTACCAGCGAAGATTATGAAAGATTACTGGAAAAATTCCACTCGGTCCAATCGGAGCTAGAACAGGAAAAAGCCCGACT
GCAAAAAGAAAAAGAACTGGCAAAACTCTCGGATCTCACTGGTATTGTCAGCAATATTGAGGTGGCCTCTGGTGCTGCTA
ACGATGAAGTGGCCGATGATCTCGGAGAAGGGGAAGCTAATCAAGCTCCCGTCATCAACCTCGTCAATAAAATTCTAGCT
AAAGCCCTACAGGAAGGGACTTCCGATATTCACATCGAACCCCAAGAAGAATTCCTGAAAATTCGCTTTCGCAAAGATGG
AGTTCTTCATCAAGCTTTTGAGCCACTGCCGAAAAAAATTACCCCGGCGGTCACTGCCCGTTTTAAAATCATGGCCGATC
TCGATATCGCCGAACGTCGTCTTCCCCAGGATGGTAAAATCCGGCGGCTTTATCAAGGGCGAACAGTGGATTTTCGGGTT
AATACCCTGCCCAGTCGCTACGGGGAAAAAGTCTGTTTACGGATTCTCGATAACTCGGCTACCCAGTTGGGTTTAGATAA
ACTGATCACCGATCAAAAGACCCTGCAAATCGTCCGAGACTTGGCCAGTCACCCTTTTGGTCTGTTACTGGTGACGGGCC
CCACCGGTTCGGGGAAATCCACCACTTTATACTCGGTGTTAGCGGAAAGAAATCACCCTGGAGTTAATATTAGTACCGCC
GAAGACCCGATCGAATATTCTCTACCAGGCATCACCCAGGTGCAGGTAATTCGGGAAAAAGGCATGGATTTCGCCTCGAT
TCTGCGGGCATTCATGCGACAAGATCCGGATGTGATTCTGGTGGGAGAAACTAGGGACAAGGAAACGGCTAAAACAGCGA
TCGAGGCCGCCCTAACTGGTCACTTGGTCCTCACTACCCTACACACCAACGATGCCGCTGGTGCGATCGCTCGTCTCGAT
AAGATGGGGGTTGAACCGTTTATGATTTCTGGTTCTCTCTTGGGAGTCTTGGCCCAGCGTCTCGTGCGGCGCGTCTGTAG
CCAATGTCGAGTCGCCTATAACCCCAGCCAAGGGGAATTAGCCCGTTTTGGTCTTGCCGCCGCCAATGAACGGGAAGTGA
CCTTTTATAAGGCTAATACCCTGACCCTAGAGGAAATTCAACAGGCGCGAAAACAGGGCAATCTCTGCCTTAAGTGTAGC
GGCAGCGGTTATAAAGGTCGCATTGGTGTCTATGAGGTAATGCAAAATAGTGAACGACTACAGCAGCTAATTTACCAAGG
GGCAACCACTGATCGCATTAAGGAGGCGGCCGTCGATGAGGGCATGGTGACACTCCTAGCCTATAGTTTAAATCTGGTGC
GCGAGGGTTACACCACCCTCGAAGAAGTGGAACGGGTGACATTTACCGATTCCGGTCTGGAAGCGGAACTAAAAGCCAAA
CGGAAAAGCGGTTTAATCTGTCGTGGTTGTCGCGCCGAACTACAACCAGAATGGCTCGATTGTCCCTATTGTATGACACC
GCGATTTAGTGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-177)

Type II/IV secretion system protein (T2SSE)

(237-504)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B0JN67

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

72.508

98.659

0.715
  pilF Thermus thermophilus HB27

40.23

90.76

0.365

Multiple sequence alignment