Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   DM992_RS05930 Genome accession   NZ_CP029824
Coordinates   1233454..1234959 (-) Length   501 a.a.
NCBI ID   WP_111016080.1    Uniprot ID   -
Organism   Burkholderia sp. JP2-270     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1228454..1239959
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DM992_RS05905 (DM992_05905) - 1229480..1230115 (-) 636 WP_111016078.1 LON peptidase substrate-binding domain-containing protein -
  DM992_RS05910 (DM992_05910) rapZ 1230143..1231051 (-) 909 WP_111016079.1 RNase adapter RapZ -
  DM992_RS05915 (DM992_05915) hprK 1231127..1232095 (-) 969 WP_006483133.1 HPr(Ser) kinase/phosphatase -
  DM992_RS05920 (DM992_05920) - 1232225..1232737 (-) 513 WP_034195643.1 PTS sugar transporter subunit IIA -
  DM992_RS05925 (DM992_05925) raiA 1232935..1233294 (-) 360 WP_006477786.1 ribosome-associated translation inhibitor RaiA -
  DM992_RS05930 (DM992_05930) rpoN/rpoN1 1233454..1234959 (-) 1506 WP_111016080.1 RNA polymerase factor sigma-54 Machinery gene
  DM992_RS05935 (DM992_05935) lptB 1235120..1235896 (-) 777 WP_111016081.1 LPS export ABC transporter ATP-binding protein -
  DM992_RS05940 (DM992_05940) lptA 1235893..1236558 (-) 666 WP_111016082.1 lipopolysaccharide transport periplasmic protein LptA -
  DM992_RS05945 (DM992_05945) lptC 1236579..1237193 (-) 615 WP_111016083.1 LPS export ABC transporter periplasmic protein LptC -
  DM992_RS05950 (DM992_05950) - 1237197..1237733 (-) 537 WP_111016084.1 HAD family hydrolase -
  DM992_RS05955 (DM992_05955) kdsD 1237733..1238716 (-) 984 WP_111016085.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54840.96 Da        Isoelectric Point: 4.8740

>NTDB_id=296230 DM992_RS05930 WP_111016080.1 1233454..1234959(-) (rpoN/rpoN1) [Burkholderia sp. JP2-270]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIVSPLRVAADGSLIAQTPSGQNTEPAP
ANNSSTSGDRAERDEPAGADEYDSYASGDGNDGPQWNLDEFGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSTGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=296230 DM992_RS05930 WP_111016080.1 1233454..1234959(-) (rpoN/rpoN1) [Burkholderia sp. JP2-270]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTGGCGCTGACGCCGCAGCTACAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCAGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGTGAGCCCGCTGCGCGTCGCTGCGGACGGATCGCTGATCGCGCAGACACCTTCCGGCCAGAACACCGAGCCGGCCCCG
GCAAACAACAGCAGCACGTCGGGCGATCGCGCGGAGCGCGACGAACCGGCCGGCGCGGACGAATACGACAGTTACGCGTC
CGGCGACGGCAACGACGGCCCGCAATGGAATCTCGACGAATTCGGCCGCTCGAGCGGAGCGTCCGACGACGACGACCTCC
CGCCGCTGCAGGTGCAGGAAGCCGCGACGTCGCTGCGCGACCACCTGTCCGCGCAGTTGCGCGTCACGCAGGCCGGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCACGCTCGACGAAGTGCT
CGCCGACCTGCCGCCCGAGCTGGAGATCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGG
CCGGAGTCGGCGCCCGCTCGGCATCGGAATGCCTGAAGCTGCAGTTGCTGCGCCTCGATCCGTCCCCGACGCGCACGCTC
GCACTCGAGATCGTGTCGCAGCATCTGGAACTGCTCGCCGCACGCGACTTCACGCGATTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGAGACGCGCACGCGCTGATCCGCTCGCTCGAGCCGTTCCCCGGCGCCGCGTACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGATGGCTCGCGGAGCTCAATCCGGAGGTCGTGCCG
CGCCTGCGGATCAACAACCTCTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGACCGGTTCGCTGAAACAGCA
GCTTCAGGAAGCACGCTGGCTGATCAAGAACATCCAGCAGCGTTTCGACACGATCCTGCGTGTCGCGCAGGCTATTGTCG
AGCGTCAAAAGAATTTCTTTGCACATGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTTTCGACCGACACCGGTGGCGCCGCCTCGTCGACCGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTCGCGCGC
CGCACGGTTGCCAAATATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAG

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.515

100

0.691

Multiple sequence alignment