Detailed information NTG0029529

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: USA300HOU_RS08220
• Genome accession: NC_010079
• Coordinates: 1654211..1654708 (-)
• Length: 165 a.a.
• NCBI ID: WP_001789864.1
• Uniprot ID: -
• Organism: Staphylococcus aureus subsp. aureus USA300_TCH1516
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1649211..1659708

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
USA300HOU_RS08195 (USA300HOU_1536)	gcvPB	1649373..1650845 (-)	1473	WP_000202189.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-
USA300HOU_RS08200 (USA300HOU_1537)	gcvPA	1650838..1652184 (-)	1347	WP_000019687.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
USA300HOU_RS08205 (USA300HOU_1538)	gcvT	1652204..1653295 (-)	1092	WP_000093349.1	glycine cleavage system aminomethyltransferase GcvT	-
USA300HOU_RS08210 (USA300HOU_1539)	-	1653454..1653978 (-)	525	WP_001015117.1	shikimate kinase	-
USA300HOU_RS08215 (USA300HOU_1540)	-	1653968..1654114 (-)	147	WP_001789879.1	hypothetical protein	-
USA300HOU_RS08220 (USA300HOU_1541)	comGF	1654211..1654708 (-)	498	WP_001789864.1	competence type IV pilus minor pilin ComGF	Machinery gene
USA300HOU_RS08225 (USA300HOU_1542)	comGE	1654626..1654925 (-)	300	WP_000844413.1	hypothetical protein	Machinery gene
USA300HOU_RS08230 (USA300HOU_1543)	comGD	1654912..1655358 (-)	447	WP_001791635.1	competence type IV pilus minor pilin ComGD	Machinery gene
USA300HOU_RS08235 (USA300HOU_1544)	comGC	1655336..1655647 (-)	312	WP_000472256.1	competence type IV pilus major pilin ComGC	Machinery gene
USA300HOU_RS08240 (USA300HOU_1545)	comGB	1655661..1656731 (-)	1071	WP_000775708.1	competence type IV pilus assembly protein ComGB	Machinery gene
USA300HOU_RS08245 (USA300HOU_1546)	comGA	1656703..1657677 (-)	975	WP_000697228.1	competence type IV pilus ATPase ComGA	Machinery gene
USA300HOU_RS08250 (USA300HOU_1547)	-	1657729..1658352 (-)	624	WP_001223008.1	MBL fold metallo-hydrolase	-
USA300HOU_RS08255 (USA300HOU_1548)	-	1658349..1658678 (-)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
USA300HOU_RS08260 (USA300HOU_1549)	-	1658678..1659664 (-)	987	WP_000161314.1	ROK family glucokinase	-

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 19164.88 Da
• Isoelectric Point: 10.2192

>NTDB_id=29529 USA300HOU_RS08220 WP_001789864.1 1654211..1654708(-) (comGF) [Staphylococcus aureus subsp. aureus USA300_TCH1516]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDI
LDDFKGVDRNDIEIRQHRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide

• Length: 498 bp

>NTDB_id=29529 USA300HOU_RS08220 WP_001789864.1 1654211..1654708(-) (comGF) [Staphylococcus aureus subsp. aureus USA300_TCH1516]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGATTTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGATGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACACCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATTAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (67-155)

• Prokaryotic N-terminal methylation motif (N_methyl) (28-51)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Staphylococcus aureus MW2	100	100	1
comGF	Staphylococcus aureus N315	96.364	100	0.964