Detailed information    

insolico Bioinformatically predicted

Overview

Name   clpE   Type   Regulator
Locus tag   EQZ01_RS07930 Genome accession   NZ_CP035231
Coordinates   1476346..1478445 (-) Length   699 a.a.
NCBI ID   WP_128737801.1    Uniprot ID   -
Organism   Bacillus subtilis strain SRCM103571     
Function   repress competence development (at the early growth phase) (predicted from homology)   
Competence regulation

Genomic Context

Location: 1471346..1483445
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQZ01_RS07895 (EQZ01_07895) spo0E 1471402..1471659 (+) 258 WP_003232480.1 aspartyl-phosphate phosphatase Spo0E -
  EQZ01_RS07900 (EQZ01_07900) - 1471745..1472176 (+) 432 WP_003232479.1 hypothetical protein -
  EQZ01_RS07905 (EQZ01_07905) kinD 1472204..1473724 (-) 1521 WP_072557174.1 sporulation kinase KinD -
  EQZ01_RS07910 (EQZ01_07910) mhqR 1473917..1474354 (+) 438 WP_003232475.1 MarR family transcriptional regulator MhqR -
  EQZ01_RS07915 (EQZ01_07915) motB 1474394..1475179 (-) 786 WP_003232473.1 flagellar motor protein MotB -
  EQZ01_RS07920 (EQZ01_07920) motA 1475151..1475963 (-) 813 WP_003238984.1 flagellar motor stator protein MotA -
  EQZ01_RS07930 (EQZ01_07930) clpE 1476346..1478445 (-) 2100 WP_128737801.1 ATP-dependent protease ATP-binding subunit ClpE Regulator
  EQZ01_RS07935 (EQZ01_07935) - 1478810..1479853 (+) 1044 WP_015383514.1 transporter -
  EQZ01_RS07940 (EQZ01_07940) queC 1480166..1480825 (+) 660 WP_032725593.1 7-cyano-7-deazaguanine synthase QueC -
  EQZ01_RS07945 (EQZ01_07945) queD 1480818..1481267 (+) 450 WP_080009794.1 6-carboxytetrahydropterin synthase QueD -
  EQZ01_RS07950 (EQZ01_07950) queE 1481260..1481991 (+) 732 WP_003232460.1 7-carboxy-7-deazaguanine synthase QueE -
  EQZ01_RS07955 (EQZ01_07955) queF 1482009..1482506 (+) 498 WP_128737802.1 preQ(1) synthase -
  EQZ01_RS07960 (EQZ01_07960) ykvN 1483025..1483381 (-) 357 WP_014417586.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 699 a.a.        Molecular weight: 77853.93 Da        Isoelectric Point: 5.1851

>NTDB_id=294666 EQZ01_RS07930 WP_128737801.1 1476346..1478445(-) (clpE) [Bacillus subtilis strain SRCM103571]
MRCQHCHQNEATIRLNMQINSVHKQMVLCETCYNELTRKPSMSMGPQSFGFPFEQAFQPKEKSAAKQSGKKGLLDELAQN
ITNGAKAGLIDPVIGRDDEVARVIEILNRRNKNNPVLIGEPGVGKTAIAEGLALKIAEGDVPNKLKNKELYLLDVASLVA
NTGIRGQFEERMKQLITELKERKNVILFIDEIHLLVGAGSAEGSMDAGNILKPALARGELQVIGATTLKEYRQIEKDAAL
ERRFQPVMVQEPSIEQAILILQGIKDKYEAYHGVTFSDEAIKACVTLSSRYIQDRHLPDKAIDLLDEAGSKANLLIDELN
DEDAAERFTAIEAEKTKALEEENYELAAKLRDEELALEKKLNSSSAHTAVTVEAEHIQEIVEQKTGIPVGKLQADEQTKM
KELEAKLHERVIGQEAAVQKVAKAVRRSRAGLKSKNRPVGSFLFVGPTGVGKTELSKTLADELFGTKDAIIRLDMSEYME
KHAVSKIIGSPPGYVGHEEAGQLTEKVRRNPYSIVLLDEIEKAHPDVQHMFLQIMEDGRLTDSQGRTVSFKDTVIIMTSN
AGAGEKQTKVGFQSDDSVVEEQTLIDSLSMFFKPEFLNRFDSIIEFRSLEKEHLVKIVSLLLGELEETLAERGISLNVTD
EAKEKIAELGYHPSFGARPLRRTIQEWVEDEMTDLLLDNGEITSFHVILEDDKIKVRAK

Nucleotide


Download         Length: 2100 bp        

>NTDB_id=294666 EQZ01_RS07930 WP_128737801.1 1476346..1478445(-) (clpE) [Bacillus subtilis strain SRCM103571]
ATGCGTTGTCAACATTGTCATCAAAACGAGGCGACGATTCGCCTTAACATGCAAATAAATTCCGTTCATAAACAGATGGT
TCTTTGTGAAACTTGCTATAACGAACTGACCCGTAAACCTTCAATGAGTATGGGTCCTCAATCTTTCGGATTTCCGTTTG
AACAGGCATTCCAGCCGAAAGAAAAGAGCGCAGCAAAACAAAGCGGAAAAAAAGGGCTGCTTGATGAGCTGGCTCAAAAT
ATTACAAACGGTGCAAAAGCCGGTCTCATTGACCCCGTCATCGGCCGTGATGATGAAGTGGCGCGAGTGATCGAAATTCT
AAACCGCCGCAACAAAAACAATCCGGTTCTTATTGGGGAGCCGGGTGTAGGGAAAACTGCCATCGCTGAAGGACTCGCTT
TAAAAATTGCTGAAGGCGATGTTCCAAACAAACTGAAAAACAAAGAGCTATATTTGCTTGATGTTGCATCCCTTGTTGCA
AACACAGGGATCAGAGGCCAATTTGAGGAGAGAATGAAACAGCTGATCACTGAGCTGAAGGAACGAAAAAATGTCATTCT
GTTCATTGATGAAATTCACCTTCTCGTCGGCGCAGGCTCTGCAGAAGGATCAATGGACGCCGGCAACATTCTCAAACCGG
CCCTAGCCAGAGGCGAACTGCAAGTCATTGGCGCGACAACACTGAAAGAATATCGTCAAATCGAAAAAGATGCCGCGCTG
GAAAGACGTTTTCAGCCTGTCATGGTGCAGGAGCCTTCAATTGAACAGGCTATCCTCATTTTGCAAGGGATTAAAGACAA
ATACGAGGCGTACCATGGCGTAACATTCAGTGATGAAGCAATCAAAGCGTGTGTCACTTTATCATCCCGCTACATTCAGG
ACAGACACCTGCCGGATAAAGCAATTGATTTATTAGATGAAGCAGGTTCAAAAGCCAACCTGTTAATTGATGAACTGAAT
GACGAGGATGCCGCTGAACGCTTTACTGCAATTGAAGCCGAAAAAACAAAAGCCCTTGAAGAAGAAAATTACGAACTGGC
GGCAAAACTCCGTGATGAAGAACTTGCATTGGAGAAAAAACTGAACAGCTCCTCCGCTCATACCGCTGTCACTGTGGAAG
CTGAGCACATTCAGGAAATTGTTGAACAAAAAACAGGCATCCCTGTCGGCAAACTGCAGGCAGACGAACAAACGAAAATG
AAAGAGCTTGAAGCAAAACTTCATGAACGCGTGATTGGACAAGAAGCCGCTGTTCAAAAAGTGGCAAAAGCGGTAAGACG
AAGCCGCGCCGGATTAAAATCCAAAAACAGACCAGTTGGCTCCTTCCTGTTCGTCGGTCCTACCGGCGTAGGGAAAACAG
AGCTTTCTAAAACACTGGCAGATGAATTATTCGGGACAAAAGACGCTATTATCCGACTCGATATGAGCGAATACATGGAG
AAACACGCCGTATCTAAAATTATCGGTTCACCGCCTGGATATGTCGGCCATGAGGAAGCTGGGCAATTAACTGAGAAAGT
GCGCCGCAATCCTTACAGCATTGTGTTGCTGGATGAGATTGAAAAAGCACACCCAGACGTTCAGCATATGTTCCTGCAAA
TTATGGAAGATGGCCGTCTGACAGACAGCCAAGGCAGAACCGTAAGCTTCAAAGACACAGTGATCATCATGACAAGTAAT
GCGGGTGCTGGTGAGAAACAAACGAAAGTCGGTTTCCAATCAGATGACAGTGTCGTCGAAGAACAAACATTAATTGATTC
ACTGAGCATGTTCTTTAAACCTGAGTTCCTCAACCGTTTTGACAGCATTATTGAGTTCCGCTCATTGGAAAAAGAACATC
TTGTCAAAATCGTCAGCCTTCTTCTTGGAGAACTTGAAGAAACATTGGCTGAACGGGGCATTAGCTTGAATGTGACAGAT
GAAGCGAAAGAAAAAATCGCTGAGCTGGGCTACCACCCTTCATTCGGTGCACGTCCGCTTAGAAGAACCATCCAAGAATG
GGTTGAGGATGAAATGACCGATCTGCTGCTTGATAATGGCGAGATCACAAGTTTTCACGTGATTTTAGAAGATGATAAAA
TCAAAGTGCGAGCGAAATAA

Domains

Predicted by InterProScan.

AAA domain (Cdc48 subfamily) (AAA_2)

(437-603)

ATPase family associated with various cellular activities (AAA) (AAA)

(116-247)

AAA lid domain (AAA_lid_9)

(253-352)

C-terminal, D2-small domain, of ClpB protein (ClpB_D2-small)

(609-679)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  clpE Streptococcus mutans UA159

56.393

100

0.599
  clpC Lactococcus lactis subsp. cremoris KW2

56.284

100

0.589
  clpE Streptococcus pneumoniae TIGR4

54.785

100

0.565
  clpE Streptococcus pneumoniae Rx1

54.785

100

0.565
  clpE Streptococcus pneumoniae D39

54.785

100

0.565
  clpE Streptococcus pneumoniae R6

54.785

100

0.565
  clpC Bacillus subtilis subsp. subtilis str. 168

53.858

90.844

0.489
  clpC Streptococcus pneumoniae Rx1

45.732

93.848

0.429
  clpC Streptococcus pneumoniae D39

45.732

93.848

0.429
  clpC Lactococcus lactis subsp. lactis strain DGCC12653

47.302

90.129

0.426
  clpC Streptococcus thermophilus LMD-9

45.427

93.848

0.426
  clpC Streptococcus thermophilus LMG 18311

44.97

93.848

0.422
  clpC Streptococcus pneumoniae TIGR4

46.85

88.555

0.415
  clpC Streptococcus mutans UA159

45.894

88.841

0.408
  clpA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

42.079

86.695

0.365

Multiple sequence alignment