Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   DLD99_RS24640 Genome accession   NZ_CP029608
Coordinates   5395514..5397103 (+) Length   529 a.a.
NCBI ID   WP_114885611.1    Uniprot ID   A0A345RW59
Organism   Pseudomonas kribbensis strain 46-2     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5390514..5402103
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DLD99_RS24620 (DLD99_24615) pgeF 5392314..5393036 (-) 723 WP_114885607.1 peptidoglycan editing factor PgeF -
  DLD99_RS24625 (DLD99_24620) rluD 5393033..5393995 (-) 963 WP_085712174.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  DLD99_RS24630 (DLD99_24625) - 5394148..5395164 (+) 1017 WP_114885609.1 outer membrane protein assembly factor BamD -
  DLD99_RS24635 (DLD99_24630) - 5395291..5395524 (+) 234 WP_085712176.1 PP0621 family protein -
  DLD99_RS24640 (DLD99_24635) pilS 5395514..5397103 (+) 1590 WP_114885611.1 ATP-binding protein Regulator
  DLD99_RS24645 (DLD99_24640) pilR 5397100..5398446 (+) 1347 WP_114885613.1 sigma-54 dependent transcriptional regulator Regulator
  DLD99_RS24650 (DLD99_24645) thiO 5398443..5399543 (-) 1101 WP_114885615.1 glycine oxidase ThiO -
  DLD99_RS24655 (DLD99_24650) - 5399601..5400002 (-) 402 WP_085712180.1 type IV pilin protein -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 58737.54 Da        Isoelectric Point: 6.5671

>NTDB_id=294076 DLD99_RS24640 WP_114885611.1 5395514..5397103(+) (pilS) [Pseudomonas kribbensis strain 46-2]
MIAEATDATRKQAQRLLRLYHLYRLSVGITLVLLISSNMDNRLLTSADDELLRNGSWLYLVLNILLVVFLENIRRPAQLF
GLALVDILLLCGLFYAAGGVASPVGNLLIVSVAISNTLLKRRIGVLIAAIGALGIVGLSFLLSFSHPLSANEYLQAGTLG
ALCFAGALLVQGLIRRLEVSETLAEQRASEVVSLEALNALILQRMRTGILVLDEQRRVQLANQSARTLLAQSPLEGHLID
DYSTALVERLNLWLNNPTLRPQSLKIAANGLELQPSFIALEQSPNRQTLVFLEDLAQIAQQAQQLKLAALGRLTAGIAHE
IRNPLGAISHAAQLLRESEELDGADQRLTQIIQDHSQRMNRVIENVLQLSRRQQSAPQRLDLEPWLEHFVEESREQASEH
QRIHLNIESGDFRTLMDPGQLTQILDNLLRNGWRHSALLHDPAQVWLRLFIDSESQLAVLEVQDNGPGVALDQQAHLFEP
FFTTSSQGTGLGLYLSRELCESNQARLDFKPRQGGGCFRITFAHGRKQS

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=294076 DLD99_RS24640 WP_114885611.1 5395514..5397103(+) (pilS) [Pseudomonas kribbensis strain 46-2]
GTGATCGCTGAGGCGACCGACGCCACTCGCAAACAGGCCCAGCGACTGCTGCGCCTCTATCACCTCTACCGTCTGAGCGT
CGGCATCACCCTGGTGCTGCTGATCTCCAGCAACATGGACAACCGCCTGCTGACGTCGGCCGACGACGAACTGCTGCGCA
ATGGCAGCTGGTTGTACCTGGTGCTGAATATCCTGCTGGTGGTGTTCCTGGAGAACATCCGCCGTCCGGCCCAGTTGTTC
GGGCTGGCCCTGGTCGACATCCTGCTGCTGTGCGGCCTGTTCTACGCCGCCGGCGGCGTGGCCAGCCCGGTCGGCAATCT
GCTGATCGTCTCGGTAGCCATCAGCAACACATTACTGAAACGCCGTATCGGCGTGCTGATTGCGGCCATCGGTGCACTCG
GCATTGTCGGTCTCAGCTTCCTGCTGAGCTTCAGTCATCCCCTGAGCGCCAACGAATACTTGCAGGCGGGCACCCTCGGC
GCTCTGTGTTTTGCCGGAGCGCTGCTGGTGCAAGGCTTGATCCGCCGCCTCGAAGTCAGCGAGACCCTCGCCGAGCAACG
GGCCAGCGAGGTGGTCAGCCTCGAGGCTCTCAACGCCTTGATCCTGCAGCGCATGCGCACCGGCATTCTGGTCCTCGACG
AACAACGCCGGGTGCAACTCGCCAACCAAAGCGCCCGGACGCTGTTGGCGCAGTCGCCCCTTGAAGGCCATTTGATCGAT
GATTATTCGACCGCGCTGGTCGAGCGCCTGAACCTGTGGCTGAACAACCCGACCCTGCGCCCGCAGAGCCTGAAAATCGC
CGCCAACGGCCTGGAACTGCAACCGAGCTTCATCGCCCTGGAGCAGAGCCCGAACCGCCAGACCCTGGTGTTTCTCGAGG
ACCTCGCGCAAATCGCCCAGCAGGCCCAGCAACTGAAGCTCGCCGCACTCGGGCGCCTGACCGCCGGCATCGCCCACGAG
ATCCGCAACCCGCTGGGTGCCATCAGTCACGCCGCACAGTTGCTGCGGGAATCCGAGGAACTCGATGGCGCGGACCAGCG
TCTGACGCAGATCATCCAGGATCACTCCCAGCGCATGAACCGAGTCATTGAAAACGTCCTGCAACTGTCCCGCCGCCAGC
AGAGCGCGCCGCAACGGCTGGATCTCGAGCCATGGCTCGAGCACTTCGTCGAGGAAAGCCGCGAACAGGCCAGCGAGCAC
CAACGGATCCATCTGAACATCGAATCCGGGGATTTCCGAACCCTGATGGACCCGGGGCAACTGACGCAAATTCTCGACAA
TCTGTTGCGCAACGGCTGGCGGCACAGCGCCCTGCTGCACGATCCGGCGCAGGTCTGGCTGCGGCTGTTCATCGACTCCG
AGAGCCAGCTGGCCGTGCTCGAAGTCCAGGACAACGGCCCCGGCGTGGCGCTGGATCAGCAGGCGCATCTGTTCGAACCC
TTCTTCACCACCAGCAGCCAGGGCACCGGCCTTGGGCTTTATCTGTCCCGTGAGCTGTGCGAAAGCAATCAGGCCCGCCT
AGACTTCAAACCACGCCAAGGCGGCGGCTGCTTTCGCATCACCTTTGCTCACGGACGGAAACAAAGTTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(309-374)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-522)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A345RW59

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

59.245

100

0.594

Multiple sequence alignment