Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   DK181_RS09110 Genome accession   NZ_CP029490
Coordinates   1861975..1863273 (-) Length   432 a.a.
NCBI ID   WP_028798478.1    Uniprot ID   -
Organism   Streptococcus sobrinus strain SL1     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1856975..1868273
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DK181_RS09075 (DK182_09075) - 1857711..1857959 (-) 249 WP_002960272.1 ASCH/PUA domain-containing protein -
  DK181_RS09080 (DK182_09080) - 1857960..1858385 (-) 426 WP_002960274.1 hypothetical protein -
  DK181_RS09090 (DK182_09090) - 1858733..1860217 (-) 1485 WP_002960276.1 primase C-terminal domain-containing protein -
  DK181_RS09095 (DK182_09095) - 1860298..1860483 (-) 186 WP_002960278.1 hypothetical protein -
  DK181_RS09100 (DK182_09100) hpf 1860683..1861231 (-) 549 WP_002960280.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  DK181_RS09105 (DK182_09105) - 1861307..1861975 (-) 669 WP_002960281.1 ComF family protein -
  DK181_RS09110 (DK182_09110) comFA/cflA 1861975..1863273 (-) 1299 WP_028798478.1 DEAD/DEAH box helicase Machinery gene
  DK181_RS09115 (DK182_09115) - 1863320..1863958 (+) 639 WP_002960285.1 YigZ family protein -
  DK181_RS09120 (DK182_09120) cysK 1864058..1864987 (+) 930 WP_019769631.1 cysteine synthase A -
  DK181_RS09125 (DK182_09125) - 1865206..1865556 (-) 351 WP_002960289.1 S1 RNA-binding domain-containing protein -
  DK181_RS09130 (DK182_09130) - 1865556..1866953 (-) 1398 WP_002960291.1 bifunctional Cof-type HAD-IIB family hydrolase/peptidylprolyl isomerase -
  DK181_RS09135 (DK182_09135) - 1867057..1867707 (-) 651 WP_019770084.1 response regulator -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49523.49 Da        Isoelectric Point: 10.1615

>NTDB_id=293176 DK181_RS09110 WP_028798478.1 1861975..1863273(-) (comFA/cflA) [Streptococcus sobrinus strain SL1]
MKDMKNYYGWQLTADQVPDDLRDKASVLPATIKKSGKYYCARCNHLLLPEWTLPDGSSYCRNCIVFGRLTTLDHLYYFEQ
KPFTKGAYLRWQGQLTPYQKEVSDILLEAVEKRENILVHAVTGSGKTEMIYRALANRLAKGKVVALVSPRIDVCRELYQR
LSRDFSCPISLLYANSEDYHRSPLLISTVHQLFKFRQAFDLIVIDEVDAFPFVDDQSLYHAVANALKPQGSKIFLTATST
DNLEKQVKKGQLREVHLARRFHANPLVSPSFVWLGDLDKKMTKGLIPVKLGQAIRQQRQTGFPLLLFYPTIVEGEQFARL
LQKYFPKEKIGFVSSRSQDRLELVQDFREGKLDILVSTTILERGVTFPKIDLFVLNAHHKLYTKSSLIQIAGRVGRSSKR
PNGLLRFYHSGVTRAMRRALQDIKLMNKKGGF

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=293176 DK181_RS09110 WP_028798478.1 1861975..1863273(-) (comFA/cflA) [Streptococcus sobrinus strain SL1]
ATGAAAGATATGAAAAATTACTATGGCTGGCAGTTGACTGCCGATCAAGTCCCGGACGACTTACGTGATAAGGCTTCGGT
CCTGCCTGCTACAATTAAAAAATCTGGGAAATATTACTGTGCTCGCTGCAACCATCTCTTATTGCCAGAGTGGACATTGC
CAGACGGTTCGTCTTATTGTCGCAACTGTATTGTTTTTGGCCGCTTGACCACTCTGGATCACCTTTATTATTTTGAGCAA
AAGCCTTTCACCAAAGGAGCTTATCTGCGTTGGCAGGGACAATTAACGCCTTACCAGAAGGAAGTTTCAGATATTCTCCT
TGAAGCAGTTGAAAAAAGAGAAAATATTTTAGTTCATGCTGTTACGGGCTCAGGTAAGACCGAGATGATTTACCGTGCTC
TAGCCAATCGACTTGCTAAAGGAAAGGTAGTCGCTTTAGTTAGCCCAAGAATTGACGTTTGCCGTGAGCTTTATCAACGT
CTTAGTCGTGATTTTAGCTGTCCGATTTCCCTCTTATATGCCAATTCAGAAGACTATCACCGCTCTCCCCTTTTGATTTC
AACCGTCCATCAACTCTTTAAATTTCGCCAGGCCTTTGACCTCATTGTGATTGACGAGGTTGATGCCTTTCCTTTTGTGG
ACGATCAGAGCCTCTACCATGCCGTAGCCAATGCCCTCAAGCCCCAAGGGTCAAAAATCTTTCTGACAGCCACCTCAACT
GATAATTTAGAGAAACAAGTCAAGAAGGGGCAACTCAGAGAGGTTCATCTAGCCCGACGCTTCCATGCCAATCCCCTTGT
CTCGCCATCCTTTGTCTGGCTGGGGGATTTGGATAAAAAGATGACCAAGGGCTTAATTCCAGTCAAGCTTGGCCAGGCTA
TCCGTCAACAGAGACAAACAGGCTTTCCGCTTTTGCTTTTTTATCCGACGATTGTTGAGGGAGAGCAGTTTGCTCGTCTC
TTACAAAAATATTTTCCCAAGGAGAAGATTGGCTTTGTCTCCAGTCGCTCCCAAGACCGCTTGGAGCTAGTTCAAGACTT
TCGAGAGGGCAAGTTAGATATCCTAGTTTCGACGACAATTCTAGAAAGAGGGGTTACCTTCCCCAAAATTGATCTCTTTG
TTTTGAATGCTCACCATAAGCTTTATACAAAATCATCCTTGATTCAAATTGCTGGCCGCGTCGGACGCAGTAGTAAGCGA
CCAAATGGCCTCCTGCGATTTTACCACTCAGGGGTGACACGAGCCATGCGACGGGCTTTGCAGGATATTAAACTGATGAA
TAAAAAAGGAGGCTTTTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(296-397)

DEAD/DEAH box helicase (DEAD)

(96-245)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis SK321

54.63

100

0.546
  comFA/cflA Streptococcus pneumoniae Rx1

53.472

100

0.535
  comFA/cflA Streptococcus pneumoniae D39

53.472

100

0.535
  comFA/cflA Streptococcus pneumoniae R6

53.472

100

0.535
  comFA/cflA Streptococcus pneumoniae TIGR4

53.241

100

0.532
  comFA/cflA Streptococcus mitis NCTC 12261

53.241

100

0.532
  comFA Lactococcus lactis subsp. cremoris KW2

48.872

92.361

0.451
  comFA Latilactobacillus sakei subsp. sakei 23K

36.092

100

0.363

Multiple sequence alignment