Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   FORC086_RS20960 Genome accession   NZ_CP029468
Coordinates   4134060..4135103 (-) Length   347 a.a.
NCBI ID   WP_001013167.1    Uniprot ID   -
Organism   Bacillus cereus strain FORC_086     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4129060..4140103
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC086_RS20915 (FORC086_21440) - 4129493..4129693 (-) 201 WP_000106083.1 YqzE family protein -
  FORC086_RS20920 (FORC086_21445) aroK 4129732..4130229 (-) 498 WP_000836624.1 shikimate kinase AroK -
  FORC086_RS20925 (FORC086_21450) - 4130345..4130995 (-) 651 WP_000183867.1 2OG-Fe(II) oxygenase -
  FORC086_RS20930 (FORC086_21455) comGG 4131170..4131541 (-) 372 WP_001231318.1 competence type IV pilus minor pilin ComGG -
  FORC086_RS20935 (FORC086_21460) comGF 4131538..4132008 (-) 471 WP_025709783.1 competence type IV pilus minor pilin ComGF -
  FORC086_RS20940 (FORC086_21465) - 4131978..4132280 (-) 303 WP_000829464.1 hypothetical protein -
  FORC086_RS20945 (FORC086_21470) comGD 4132273..4132728 (-) 456 WP_000813141.1 competence type IV pilus minor pilin ComGD -
  FORC086_RS20950 (FORC086_21475) comGC 4132725..4133024 (-) 300 WP_001178684.1 competence type IV pilus major pilin ComGC -
  FORC086_RS20955 (FORC086_21480) comGB 4133036..4134073 (-) 1038 WP_281953682.1 competence type IV pilus assembly protein ComGB -
  FORC086_RS20960 (FORC086_21485) comGA 4134060..4135103 (-) 1044 WP_001013167.1 competence type IV pilus ATPase ComGA Machinery gene
  FORC086_RS20965 (FORC086_21490) - 4135309..4136004 (+) 696 WP_000434125.1 helix-turn-helix domain-containing protein -
  FORC086_RS20970 (FORC086_21495) - 4136130..4136372 (+) 243 WP_000440716.1 DUF2626 domain-containing protein -
  FORC086_RS20975 (FORC086_21500) - 4136480..4137874 (+) 1395 WP_025709781.1 L-cystine transporter -
  FORC086_RS20980 (FORC086_21505) - 4137954..4138346 (-) 393 WP_000875399.1 hypothetical protein -
  FORC086_RS20985 (FORC086_21510) - 4138434..4138649 (-) 216 WP_001008327.1 DUF3912 family protein -
  FORC086_RS20990 (FORC086_21515) - 4138902..4139213 (+) 312 WP_001093237.1 hypothetical protein -
  FORC086_RS20995 (FORC086_21520) - 4139248..4139736 (-) 489 WP_000764267.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39092.47 Da        Isoelectric Point: 8.3536

>NTDB_id=292882 FORC086_RS20960 WP_001013167.1 4134060..4135103(-) (comGA) [Bacillus cereus strain FORC_086]
MNGIEIFANAILKEACRVQASDLHIVPRQKDVAVQLRIGKDLMTIQCIEKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFIDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQALKEADGEC
VTYKHETLESSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=292882 FORC086_RS20960 WP_001013167.1 4134060..4135103(-) (comGA) [Bacillus cereus strain FORC_086]
ATGAATGGGATTGAAATTTTTGCGAATGCAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCGGTGCAACTACGTATAGGAAAAGATTTAATGACGATACAGTGTATTGAAAAGGAGTTTGGAG
AAAAACTTGTCTCACATTTCAAATTTTTAGCATCTATGGACATAGGCGAGAAACGGAAGCCACAGAATGGTTCGTTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCCACACTTCCAACAGTATATCAAGAAAGTCTTGTTATTCGTCT
TCATTTACAAGCATCTGTTCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTTGTGTTTACTGGACCGACTGGATCTGGGAAGACGACGACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGCATCATTACACTTGAAGATCCGGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTCACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATCCGTGATGAAGAAACAGCAAAAATAGCTATAAGAGCAAGTTTGACAGGACATTTAGTAATGACAACGCTGCAT
ACGAATGATGCGAAAGGAGCAATCATTCGTTTCATAGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAGTGTCCGTTTTGCAGAGGAAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAGGCTAGCATTTATGAGCTACTATATGGATATGAGTTAAAACAAGCGCTTAAAGAAGCTGATGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCATCAATACGAAAAGGATATGCTTTAGGTTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.908

100

0.559
  pilB Haemophilus influenzae 86-028NP

39.752

92.795

0.369

Multiple sequence alignment