Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   BPUM_RS11030 Genome accession   NC_009848
Coordinates   2225551..2226621 (-) Length   356 a.a.
NCBI ID   WP_012010567.1    Uniprot ID   A8FF57
Organism   Bacillus pumilus SAFR-032     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2220551..2231621
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BPUM_RS10980 (BPUM_2195) sipW 2220643..2221215 (-) 573 WP_012010557.1 signal peptidase I SipW -
  BPUM_RS10985 (BPUM_2196) tapA 2221267..2221794 (-) 528 WP_012010558.1 amyloid fiber anchoring/assembly protein TapA -
  BPUM_RS10990 (BPUM_2197) - 2222094..2222372 (+) 279 WP_061827416.1 DUF3889 domain-containing protein -
  BPUM_RS10995 (BPUM_2198) - 2222407..2222601 (-) 195 WP_012010560.1 YqzE family protein -
  BPUM_RS11000 (BPUM_2199) - 2222661..2223044 (-) 384 WP_012010561.1 competence type IV pilus minor pilin ComGG -
  BPUM_RS11005 (BPUM_2200) comGF 2223041..2223541 (-) 501 WP_041815716.1 competence type IV pilus minor pilin ComGF -
  BPUM_RS11010 (BPUM_2201) comGE 2223480..2223797 (-) 318 WP_012010563.1 competence type IV pilus minor pilin ComGE -
  BPUM_RS11015 (BPUM_2202) comGD 2223781..2224227 (-) 447 WP_041815719.1 competence type IV pilus minor pilin ComGD -
  BPUM_RS11020 (BPUM_2203) comGC 2224220..2224513 (-) 294 WP_012010565.1 competence type IV pilus major pilin ComGC Machinery gene
  BPUM_RS11025 (BPUM_2204) comGB 2224530..2225609 (-) 1080 WP_225970031.1 competence type IV pilus assembly protein ComGB -
  BPUM_RS11030 (BPUM_2205) comGA 2225551..2226621 (-) 1071 WP_012010567.1 competence type IV pilus ATPase ComGA Machinery gene
  BPUM_RS11035 (BPUM_2206) - 2226799..2227671 (-) 873 WP_012010568.1 STAS domain-containing protein -
  BPUM_RS11045 (BPUM_2207) - 2227894..2228274 (-) 381 WP_003217382.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  BPUM_RS11050 (BPUM_2208) - 2228488..2228730 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  BPUM_RS11055 (BPUM_2209) - 2228777..2229415 (-) 639 WP_041815722.1 MBL fold metallo-hydrolase -
  BPUM_RS18815 (BPUM_2210) - 2229573..2229746 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  BPUM_RS11060 (BPUM_2211) - 2229971..2230285 (-) 315 WP_012010571.1 MTH1187 family thiamine-binding protein -
  BPUM_RS11065 (BPUM_2212) - 2230364..2231617 (-) 1254 WP_041815725.1 MFS transporter -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40254.65 Da        Isoelectric Point: 9.0692

>NTDB_id=29287 BPUM_RS11030 WP_012010567.1 2225551..2226621(-) (comGA) [Bacillus pumilus SAFR-032]
MYGIEYLGQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRTIDKKSGERLIAHFKFVSSMDIGEKRRPQNGSLA
VMLRSGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASTRLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMIELEQTMVAIAAQRLIDLTCPFCGETCQLYCKLNRPVRRTNVFELLFGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=29287 BPUM_RS11030 WP_012010567.1 2225551..2226621(-) (comGA) [Bacillus pumilus SAFR-032]
TTGTATGGGATTGAATATTTAGGACAAGAGCTGCTAGAAGAAGCATGCCGCATGAGAGCATCTGATGTGCACATTGTTCC
AAAGGAAAAGGAAGCGTCGGTATCTTTCCGCGTAGACTCAGACTTAATTCAGCAAAGAACTATTGATAAAAAAAGCGGCG
AGCGACTCATTGCTCATTTTAAATTTGTATCTTCTATGGATATTGGGGAAAAAAGGAGACCTCAAAATGGGTCACTAGCC
GTGATGCTAAGAAGCGGTCAGGTGTTTATTCGAATGTCTACCTTACCAACTGTAAATGATGAGAGTTTAGTGATTAGAAT
ATTGCCACAGGATCATGTTCCGAAAATAAAGCATCTGTCACTATTTCCGAAAGCCTCCACCAGATTATTATCGTTTTTGA
ATCATTCGCATGGGCTGATTTTATTTACCGGTCCAACCAATTCAGGGAAAACAACAACCCTCTATTCATTGATCCAGTTT
GCAAAAAAGAATTTCAATCGAAATATTATTACACTTGAAGATCCAGTAGAAACGAGAAATGAAGAAGTGTTACAGGTTCA
AGTAAATGAAAAAGCCGGCATCACATATGCCGCAGGATTACGTGCTATTTTAAGGCATGATCCAGATATGATTGTGCTAG
GAGAAATAAGAGATGCTGAAACGGCACGAACGGCAATTAGAGCAGCGCTGACAGGCCATTTAGTGATGAGTACACTCCAT
GCGAAAAATGCAAAAGGAGCCCTTTATCGTATGCTTGAATTTGGTGTCACAATGATTGAACTTGAACAAACAATGGTGGC
GATTGCTGCGCAGCGATTAATTGATCTCACCTGTCCTTTTTGCGGAGAAACATGTCAGCTTTACTGTAAATTAAATCGAC
CGGTCAGACGAACAAATGTATTTGAACTGCTGTTCGGAAAAGAGCTTGGTGAGTGTATCAAGGAGGCGAAAGGAGAATAT
GCTCACTCGTCATATGAAACACTGCAAAGATTAATTCGTAAAGGAGTGGCACTTGGCTATTTATCGAAAAACACCTATCA
TCGCTGGGTTTATGAAGAAGCAAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A8FF57

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.124

99.438

0.638

Multiple sequence alignment