Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   DKG78_RS02230 Genome accession   NZ_CP029466
Coordinates   407251..407688 (-) Length   145 a.a.
NCBI ID   WP_132106741.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain HM618     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 402251..412688
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DKG78_RS02205 (DKG78_02235) - 403270..404406 (+) 1137 WP_025284207.1 zinc-dependent alcohol dehydrogenase -
  DKG78_RS02210 (DKG78_02240) - 404421..404855 (+) 435 WP_007409358.1 RDD family protein -
  DKG78_RS02215 (DKG78_02245) - 404973..405251 (+) 279 WP_372691166.1 YckD family protein -
  DKG78_RS02220 (DKG78_02250) - 405355..406791 (+) 1437 WP_076425807.1 glycoside hydrolase family 1 protein -
  DKG78_RS02225 (DKG78_02255) nin/comJ 406832..407230 (-) 399 WP_014416873.1 competence protein ComJ Regulator
  DKG78_RS02230 (DKG78_02260) nucA/comI 407251..407688 (-) 438 WP_132106741.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  DKG78_RS02235 (DKG78_02265) hxlB 408047..408604 (-) 558 WP_007409354.1 6-phospho-3-hexuloisomerase -
  DKG78_RS02240 (DKG78_02270) hxlA 408601..409236 (-) 636 WP_015239130.1 3-hexulose-6-phosphate synthase -
  DKG78_RS02245 (DKG78_02275) - 409468..409830 (+) 363 WP_012116770.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16042.33 Da        Isoelectric Point: 8.4850

>NTDB_id=292782 DKG78_RS02230 WP_132106741.1 407251..407688(-) (nucA/comI) [Bacillus amyloliquefaciens strain HM618]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSGICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=292782 DKG78_RS02230 WP_132106741.1 407251..407688(-) (nucA/comI) [Bacillus amyloliquefaciens strain HM618]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCTTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCGGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCCGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAACGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCATGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATTAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment