Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   DKE43_RS07420 Genome accession   NZ_CP029464
Coordinates   1416699..1417769 (-) Length   356 a.a.
NCBI ID   WP_060596836.1    Uniprot ID   -
Organism   Bacillus pumilus strain ZB201701     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1411699..1422769
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DKE43_RS07370 (DKE43_07390) - 1411793..1412365 (-) 573 WP_066031752.1 signal peptidase I -
  DKE43_RS07375 (DKE43_07395) tapA 1412417..1412944 (-) 528 WP_127687049.1 amyloid fiber anchoring/assembly protein TapA -
  DKE43_RS07380 (DKE43_07400) - 1413244..1413522 (+) 279 WP_223250494.1 DUF3889 domain-containing protein -
  DKE43_RS07385 (DKE43_07405) - 1413556..1413750 (-) 195 WP_024718846.1 YqzE family protein -
  DKE43_RS07390 (DKE43_07410) comGG 1413809..1414192 (-) 384 WP_034661288.1 competence type IV pilus minor pilin ComGG -
  DKE43_RS07395 (DKE43_07415) comGF 1414189..1414689 (-) 501 WP_034661289.1 competence type IV pilus minor pilin ComGF -
  DKE43_RS07400 (DKE43_07420) comGE 1414628..1414945 (-) 318 WP_034661291.1 competence type IV pilus minor pilin ComGE -
  DKE43_RS07405 (DKE43_07425) comGD 1414929..1415375 (-) 447 WP_066030666.1 competence type IV pilus minor pilin ComGD -
  DKE43_RS07410 (DKE43_07430) comGC 1415368..1415661 (-) 294 WP_003217322.1 competence type IV pilus major pilin ComGC Machinery gene
  DKE43_RS07415 (DKE43_07435) comGB 1415678..1416718 (-) 1041 WP_060596835.1 competence type IV pilus assembly protein ComGB -
  DKE43_RS07420 (DKE43_07440) comGA 1416699..1417769 (-) 1071 WP_060596836.1 competence type IV pilus ATPase ComGA Machinery gene
  DKE43_RS07425 (DKE43_07450) - 1417947..1418819 (-) 873 WP_034661297.1 STAS domain-containing protein -
  DKE43_RS07435 (DKE43_07460) - 1419043..1419423 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  DKE43_RS07440 (DKE43_07465) - 1419637..1419879 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  DKE43_RS07445 (DKE43_07470) - 1419928..1420566 (-) 639 WP_060596838.1 MBL fold metallo-hydrolase -
  DKE43_RS07455 (DKE43_07480) - 1420724..1420897 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  DKE43_RS07460 (DKE43_07485) - 1420922..1421236 (-) 315 WP_034662003.1 MTH1187 family thiamine-binding protein -
  DKE43_RS07465 (DKE43_07490) - 1421314..1422567 (-) 1254 WP_060596687.1 MFS transporter -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40267.63 Da        Isoelectric Point: 9.0692

>NTDB_id=292664 DKE43_RS07420 WP_060596836.1 1416699..1417769(-) (comGA) [Bacillus pumilus strain ZB201701]
MYGIEYLGQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRSGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASIRLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDLIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELTCPFCGETCQLYCKLNRSVRRTNVFELLFGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=292664 DKE43_RS07420 WP_060596836.1 1416699..1417769(-) (comGA) [Bacillus pumilus strain ZB201701]
TTGTATGGTATTGAATATTTGGGACAAGAGCTGCTAGAAGAAGCATGCCGCATGAGAGCATCTGATGTGCATATTGTTCC
AAAAGAAAAGGAAGCGTCGGTATCTTTCCGCGTAGACTCAGATTTAATTCAGCAGCGAACCATTGATAAAAAAAGCGGCG
AGCGGCTCATTGCTCATTTTAAATTTTTATCATCCATGGATATCGGGGAAAAAAGGAGGCCGCAAAATGGGTCACTAGCT
GTGATGCTAAGAAGCGGCCAGGTGTTTATTCGGATGTCTACCTTACCAACTGTAAATGATGAGAGTTTAGTGATTAGAAT
ATTGCCGCAGGATCATGTTCCAAAAATAAAACATCTGTCACTATTTCCGAAAGCCTCCATCAGATTATTATCATTTTTGA
ATCATTCGCATGGGCTGATTTTATTTACCGGTCCAACAAATTCAGGGAAAACAACAACCCTTTATTCATTAATCCAGTTT
GCAAAAAAGAATTTCAACCGAAATATTATTACACTTGAAGATCCTGTGGAAACAAGAAATGAAGAAGTGTTACAGGTTCA
AGTGAATGAAAAAGCCGGCATCACATATGCCGCAGGATTACGTGCTATTTTAAGACATGATCCAGATCTGATTGTGCTAG
GAGAAATAAGAGATGCCGAAACGGCCCGAACGGCAATTAGAGCAGCACTGACAGGCCATTTAGTGATGAGTACGCTTCAT
GCGAAAAATGCAAAAGGAGCCCTTTATCGTATGCTTGAATTTGGTGTCACGATGAATGAACTCGAACAAACGATGGTTGC
GATTGCTGCGCAGCGATTAATCGAGCTCACCTGTCCTTTTTGCGGAGAAACATGTCAGCTTTACTGTAAATTAAATCGAT
CGGTCAGACGAACAAATGTATTTGAACTGCTGTTCGGAAAGGAGCTAGGTGAGTGTATCAAGGAGGCTAAAGGAGAATAT
GCTCACTCGTCATATGAAACACTGCAAAGATTAATTCGGAAAGGAGTGGCACTAGGCTATTTATCGAAAAACACCTATCA
TCGCTGGGTTTATGAAGAAGCAAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.124

99.438

0.638

Multiple sequence alignment