Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC   Type   Machinery gene
Locus tag   DKE43_RS07410 Genome accession   NZ_CP029464
Coordinates   1415368..1415661 (-) Length   97 a.a.
NCBI ID   WP_003217322.1    Uniprot ID   -
Organism   Bacillus pumilus strain ZB201701     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1410368..1420661
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DKE43_RS07360 (DKE43_07380) - 1410533..1410859 (+) 327 Protein_1440 helix-turn-helix domain-containing protein -
  DKE43_RS07365 (DKE43_07385) - 1410935..1411735 (-) 801 WP_034661283.1 TasA family protein -
  DKE43_RS07370 (DKE43_07390) - 1411793..1412365 (-) 573 WP_066031752.1 signal peptidase I -
  DKE43_RS07375 (DKE43_07395) tapA 1412417..1412944 (-) 528 WP_127687049.1 amyloid fiber anchoring/assembly protein TapA -
  DKE43_RS07380 (DKE43_07400) - 1413244..1413522 (+) 279 WP_223250494.1 DUF3889 domain-containing protein -
  DKE43_RS07385 (DKE43_07405) - 1413556..1413750 (-) 195 WP_024718846.1 YqzE family protein -
  DKE43_RS07390 (DKE43_07410) comGG 1413809..1414192 (-) 384 WP_034661288.1 competence type IV pilus minor pilin ComGG -
  DKE43_RS07395 (DKE43_07415) comGF 1414189..1414689 (-) 501 WP_034661289.1 competence type IV pilus minor pilin ComGF -
  DKE43_RS07400 (DKE43_07420) comGE 1414628..1414945 (-) 318 WP_034661291.1 competence type IV pilus minor pilin ComGE -
  DKE43_RS07405 (DKE43_07425) comGD 1414929..1415375 (-) 447 WP_066030666.1 competence type IV pilus minor pilin ComGD -
  DKE43_RS07410 (DKE43_07430) comGC 1415368..1415661 (-) 294 WP_003217322.1 competence type IV pilus major pilin ComGC Machinery gene
  DKE43_RS07415 (DKE43_07435) comGB 1415678..1416718 (-) 1041 WP_060596835.1 competence type IV pilus assembly protein ComGB -
  DKE43_RS07420 (DKE43_07440) comGA 1416699..1417769 (-) 1071 WP_060596836.1 competence type IV pilus ATPase ComGA Machinery gene
  DKE43_RS07425 (DKE43_07450) - 1417947..1418819 (-) 873 WP_034661297.1 STAS domain-containing protein -
  DKE43_RS07435 (DKE43_07460) - 1419043..1419423 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  DKE43_RS07440 (DKE43_07465) - 1419637..1419879 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  DKE43_RS07445 (DKE43_07470) - 1419928..1420566 (-) 639 WP_060596838.1 MBL fold metallo-hydrolase -

Sequence

Protein


Download         Length: 97 a.a.        Molecular weight: 10739.69 Da        Isoelectric Point: 7.3837

>NTDB_id=292663 DKE43_RS07410 WP_003217322.1 1415368..1415661(-) (comGC) [Bacillus pumilus strain ZB201701]
MNDKGFTLLEMLIVMLVISILLLITIPNITRHHQTIQAKGCEGLKSMVSTQVMAYELEHDGKTPSLAELEKEGYVKKGLT
CPNGKAIVIQNGHVKHE

Nucleotide


Download         Length: 294 bp        

>NTDB_id=292663 DKE43_RS07410 WP_003217322.1 1415368..1415661(-) (comGC) [Bacillus pumilus strain ZB201701]
ATGAATGATAAAGGATTTACCCTGTTAGAAATGTTAATTGTCATGCTCGTGATTTCGATTCTTTTACTTATTACCATCCC
GAATATCACTCGTCACCACCAAACGATTCAAGCAAAGGGCTGTGAAGGGCTTAAATCAATGGTGAGCACACAGGTGATGG
CATATGAATTAGAGCATGATGGCAAGACACCATCTTTAGCTGAATTAGAAAAAGAAGGCTATGTCAAAAAGGGATTAACG
TGTCCTAATGGAAAAGCGATTGTCATTCAAAACGGCCATGTGAAGCATGAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

68.041

100

0.68
  comGC Staphylococcus aureus MW2

41.304

94.845

0.392
  comGC Staphylococcus aureus N315

41.304

94.845

0.392

Multiple sequence alignment