Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   BPUM_RS05120 Genome accession   NC_009848
Coordinates   1076846..1080550 (+) Length   1234 a.a.
NCBI ID   WP_012009495.1    Uniprot ID   -
Organism   Bacillus pumilus SAFR-032     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1071846..1085550
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BPUM_RS05105 (BPUM_04100) - 1072141..1072551 (+) 411 WP_025093362.1 DoxX family protein -
  BPUM_RS05110 (BPUM_04105) - 1072748..1073194 (-) 447 WP_041815371.1 ferritin-like domain-containing protein -
  BPUM_RS05115 (BPUM_0993) addB 1073359..1076868 (+) 3510 WP_041815374.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  BPUM_RS05120 (BPUM_0994) addA 1076846..1080550 (+) 3705 WP_012009495.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  BPUM_RS05125 (BPUM_0995) sbcD 1080577..1081752 (+) 1176 WP_012009496.1 exonuclease subunit SbcD -
  BPUM_RS05130 (BPUM_0996) - 1081749..1085144 (+) 3396 WP_012009497.1 AAA family ATPase -
  BPUM_RS05135 (BPUM_0997) - 1085164..1085463 (+) 300 WP_012009498.1 hypothetical protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 142263.29 Da        Isoelectric Point: 5.0649

>NTDB_id=29265 BPUM_RS05120 WP_012009495.1 1076846..1080550(+) (addA) [Bacillus pumilus SAFR-032]
MQIPKPNNSTWTDDQWEAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEHPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELAKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPS
FFELVDRYTSDRHDLDLQWLVKRIYDFSRSHPSPEQWMRAFLSLYDVDAQTKVEELPFYPYIKEDLSLVLRSCQELLERA
LSLSKEPGGPAPRAENFIDDLEQVNELIRHQDDFEKLYELLPNVNFKRLKTCKGDEYDPVLLEKATDARNQAKKQLEKLK
DEYFMRSPAQHLKSLAEMKPIVETLVELVIQFGERFERAKQEKSIVDFSDLEHYCLRILAEQDAEGHLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGEHSSEGNLFMVGDVKQSIYRFRLAEPMLFLNKYKHFQPDGKETGKRIDLNKNFR
SRSDVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPESKDTTTEMLLVHLDQQEAETGEEREELETVQFEARIIAKK
IKELVEQPFQVYDAKQQMTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPLVHLDENELALIRTSDKKGTYYDAVKAFMSVTHSDHPTCKKLERFFQLLRKWRDFSINHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPVVFTVGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLVAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAATGEEWLLPDFERYQSKTYLDFIGPALIRHQAMSSVLEETGDVVLSHPSTFTISFTQASDL
LKEDMSLEKKEQDEIVQALMDGLPVEGYGDADEKVAERLSWKYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPSSIRKAR
ATLYDRPAFMKKKTLTAAEQGTAMHTVMQHIPLPSEEPYDESRIGHLLDSLQQRDLLTDEQVQSINQEGIAAFFSTSIGQ
KLLKADWVKREVSFSMVLPVKEVYSHIDTEGEPVLIQGMIDCLFETDGKLYLLDYKTDRVQGRYTGGIDAAVPILKKRYE
TQIALYAKAVERLTNRTLEEKILYFFDGNVEISL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=29265 BPUM_RS05120 WP_012009495.1 1076846..1080550(+) (addA) [Bacillus pumilus SAFR-032]
ATGCAAATACCAAAACCGAATAACAGTACGTGGACGGATGACCAGTGGGAAGCCATCGTTTCAGAAGGACAAGATATCTT
AGTTGCGGCGGCGGCAGGTTCAGGTAAAACAGCTGTTTTGGTTGAACGCCTGATTCGAAAGATGACCCGGCCTGAACATC
CAGTTGATGTCGATCGTCTGCTCGTTGTGACTTTCACAAATGCATCTGCAGCAGAGATGAAGCATCGGATCACAGAAGCA
CTTGAAAAAGAATTAGCCAAAAATCCTGGGTCTCTTCATATGAGAAGGCAGCTGTCCCTCATGAATCGAGCCAATATTTC
AACCTTGCACTCCTTTTGCTTACAGGTTTTACGGACCTTTTACTATGAAATTGATCTTGATCCAGGCTTCCGTTTGGCTG
ATCAAACAGAAGGAGAACTATTAGGAGATGAAGTGTTAGACGAACTGTTTGAGGATGAATATAAGGCTGGGAAGCCATCA
TTCTTTGAATTGGTTGATCGTTACACGTCTGACCGTCATGACTTAGATTTGCAATGGCTCGTGAAGAGAATCTATGATTT
TTCAAGATCTCATCCTTCTCCGGAGCAATGGATGCGGGCATTCCTTTCACTATATGATGTAGATGCTCAAACAAAAGTAG
AAGAATTACCGTTTTATCCCTATATCAAAGAAGATCTTTCGCTCGTTCTCCGGAGCTGCCAGGAGCTGCTTGAACGAGCT
CTTTCGCTATCAAAAGAGCCCGGCGGTCCAGCACCGAGAGCAGAGAATTTTATAGATGATTTAGAGCAAGTCAATGAATT
AATCCGTCATCAAGATGATTTTGAGAAACTATATGAGCTTTTACCGAACGTCAATTTTAAAAGGCTTAAAACGTGCAAAG
GGGACGAATATGACCCTGTTTTATTAGAGAAAGCTACTGATGCACGTAATCAAGCAAAAAAACAATTAGAAAAACTAAAA
GATGAGTACTTCATGCGCAGTCCTGCCCAGCATTTAAAAAGCTTAGCTGAAATGAAGCCGATTGTCGAGACACTTGTAGA
GCTAGTGATCCAATTTGGTGAGCGCTTCGAAAGAGCGAAGCAGGAAAAGTCTATTGTCGATTTTTCCGATTTAGAGCACT
ATTGCTTACGCATTTTAGCGGAGCAGGATGCAGAAGGACATTTGATCGAAACAGAAGCTGCTAAGTACTATCAACAGCAG
TTTGAAGAAGTGCTCGTTGATGAATACCAGGATACAAACCTTGTACAAGAAACAATTTTAAAACTTGTATCTAAAGGAGA
ACATTCTTCAGAGGGTAATCTGTTTATGGTTGGTGATGTCAAGCAGTCTATTTATCGTTTTAGACTGGCTGAGCCCATGC
TCTTTTTAAACAAATATAAACACTTTCAACCAGACGGCAAAGAAACAGGAAAGAGAATCGATTTAAATAAAAACTTCCGC
AGCCGTTCAGATGTTTTAGATAGTACGAACTTTTTGTTCAAGCAGCTGATGGGGGAAACAGTTGGAGAAATTGAATATGA
TGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCAGAAAGCAAAGATACAACGACAGAAATGCTTCTTGTCCACTTAG
ATCAGCAGGAAGCAGAAACGGGCGAAGAGCGAGAAGAGCTCGAGACCGTCCAATTTGAAGCGAGAATCATTGCTAAAAAA
ATAAAAGAATTAGTTGAGCAGCCATTTCAAGTGTACGATGCAAAGCAGCAAATGACCCGGAACTTGCAGTATCGAGACAT
TGTCATTTTACTTCGGTCTATGCCGTGGGCTCCGCAAATGATGGAGGAATTAAAGAAGCAGGGAATCCCCGTATATGCGA
ATCTTTCCTCTGGTTATTTTGAAGCAACAGAGGTGTCTGTCATCCTTTCTTTGTTAAAAGTGATCGATAATCCATATCAG
GATATCCCGCTTGCTGCTGTTTTAAGATCACCTCTTGTTCATTTAGATGAAAATGAGTTGGCACTGATTCGGACGAGTGA
TAAAAAAGGCACGTATTATGATGCTGTAAAGGCCTTTATGAGTGTGACTCATTCTGATCATCCTACATGCAAAAAGCTAG
AGCGATTTTTCCAATTACTGCGCAAATGGCGTGATTTCTCCATCAATCATTCGGTGGCAGAATTAATTTGGGAAGTCTAC
CGCGATACACAGTATCTTGATTATGTTGGCGGAATGCCTGGAGGTAAGCAGCGGCAAGCGAATCTTCGTGCCCTTTATGA
CCGGGCCAAGCAGTACGAAAAAGCTGCCTTTAGAGGGCTATTCCGATTCCTTCGTTTTATTGAAAGAATGCAGGAGCGTG
GGGACGATCTTGGTGCGGCAAAAACGTTCAGTGAAACAGAGGACGTTGTGCGCATGATGACGATTCATAGTAGTAAAGGA
TTAGAATTCCCAGTCGTCTTTACCGTAGGACTTGGCAGAAACTTTAATATGATGGACTTAAACCAGTCTTATTTACTTGA
TAAAGAACTAGGCTTTGGAAGCAAGTATATCCATCCTGAGCTGAGAATTAGTTATGCGACTTTACCACTTGTGGCGATGA
AAAAAAAGATGAGAAAAGAGTTGTTATCTGAAGAGCTTAGAGTGCTTTATGTGGCGCTCACAAGGGCTAAAGAAAAATTA
TTTCTCGTCGGCTCTGTCAAAAATCAGGTGAAAGCATTAAGCAAATGGCAAAATGCCGCAACCGGAGAAGAGTGGCTGCT
CCCAGATTTTGAACGGTATCAATCGAAAACATACTTAGATTTCATTGGACCTGCCCTCATCCGTCATCAAGCGATGTCAT
CTGTTTTAGAAGAAACAGGGGATGTTGTTCTTTCGCATCCGTCTACATTCACTATCTCATTTACGCAAGCTTCTGATCTT
TTAAAGGAAGATATGTCACTTGAAAAGAAAGAGCAAGACGAGATTGTACAAGCATTGATGGATGGTCTACCTGTTGAAGG
ATATGGCGATGCTGATGAGAAGGTGGCAGAGAGGCTCTCGTGGAAATATCCGTATCTAGCGGCCTCACAAGTCGGCACAA
AGCAGTCTGTTTCCGAGATAAAAAGAATGAAAGAAATACAGGATGAGTACAGTGTTCCTTCTTCTATTCGAAAAGCTCGT
GCAACGTTGTATGACCGGCCGGCTTTCATGAAGAAAAAAACACTCACTGCCGCAGAACAAGGCACCGCCATGCATACAGT
GATGCAGCATATCCCGCTTCCTTCTGAGGAGCCCTATGATGAGTCACGTATAGGACACTTGCTCGATTCTTTACAACAGC
GAGACCTGCTAACAGATGAGCAGGTTCAGTCAATTAATCAAGAGGGAATCGCTGCTTTCTTCTCTACTTCAATTGGTCAA
AAACTGCTAAAAGCGGACTGGGTGAAACGAGAGGTGTCATTTAGTATGGTGTTACCTGTTAAGGAGGTATACAGTCATAT
TGATACTGAGGGAGAGCCTGTTCTAATTCAAGGAATGATTGACTGTTTATTTGAAACAGATGGAAAGCTATATTTACTAG
ATTATAAAACTGATAGAGTTCAAGGCAGATATACAGGTGGAATTGACGCTGCTGTACCAATCTTGAAAAAGCGTTATGAA
ACCCAAATCGCGTTATATGCAAAAGCGGTAGAGCGTTTGACAAACAGAACATTAGAGGAGAAAATTCTTTATTTCTTCGA
TGGAAATGTAGAGATATCTTTATAA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(473-883)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-463)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1052-1225)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

66.1

100

0.662

Multiple sequence alignment