Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   BPUM_RS05115 Genome accession   NC_009848
Coordinates   1073359..1076868 (+) Length   1169 a.a.
NCBI ID   WP_041815374.1    Uniprot ID   -
Organism   Bacillus pumilus SAFR-032     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1068359..1081868
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BPUM_RS05085 (BPUM_04090) lepB 1068869..1069381 (+) 513 WP_041815362.1 signal peptidase I -
  BPUM_RS05090 (BPUM_0991) - 1069417..1070562 (-) 1146 WP_012009492.1 AbrB family transcriptional regulator -
  BPUM_RS05095 (BPUM_04095) - 1070829..1071251 (+) 423 WP_041815366.1 BlaI/MecI/CopY family transcriptional regulator -
  BPUM_RS05100 (BPUM_0992) - 1071244..1072083 (+) 840 WP_012009493.1 M56 family metallopeptidase -
  BPUM_RS05105 (BPUM_04100) - 1072141..1072551 (+) 411 WP_025093362.1 DoxX family protein -
  BPUM_RS05110 (BPUM_04105) - 1072748..1073194 (-) 447 WP_041815371.1 ferritin-like domain-containing protein -
  BPUM_RS05115 (BPUM_0993) addB 1073359..1076868 (+) 3510 WP_041815374.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  BPUM_RS05120 (BPUM_0994) addA 1076846..1080550 (+) 3705 WP_012009495.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  BPUM_RS05125 (BPUM_0995) sbcD 1080577..1081752 (+) 1176 WP_012009496.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1169 a.a.        Molecular weight: 134855.78 Da        Isoelectric Point: 5.1634

>NTDB_id=29264 BPUM_RS05115 WP_041815374.1 1073359..1076868(+) (addB) [Bacillus pumilus SAFR-032]
MEIQFLAGRSGSGKTTAILEEIKEQLRLDPLGPPIIFLVPDQMTFLMEYELAKTSEAGGMIRAKVFSFTRLAWSILQQTG
GANRQFVTSTGIQMLLRKVIEEQKDKFKVFKKASDKPGFVEQIEKTMAEFKRYCMLPEEIEKISVESMLSEYTEERRAAE
KLHDLHVLYQQMEEHLQDEYVHSEDYLNLLAQQIPSAEEIKGAHIYIDGFYQFTPQQLLVIEQLLLHAAKVTAAFTVDQS
YHDRQPNELDLFRMTGKTYFQLYQLAKECGADISETIFERNHRHLYTPDLAYLEHQYEQRPVQPYQENTPHLTVSKSASK
RAEIEGVARDILDLVREKGLRLRDISVVARHVDDYKDTLKEVFRDYDIPFFIDGNESMQYHPLIELIRSSLDVIKGNWRY
EAVFRCVKTEFLFPLEITKNKAREQADQLENYCIAYGVKGERWTNGSRFHYRRFQSLDEDFRQTDQEIEMEQMLNDVKEW
ITPPLYQLQKRLKNAQKVRDMVEAVYVFLEEIQVPDKLEKARLEAEEAGRLAEAMQHGQVWDAVIQLMDEFVDMLGDEEL
SFPLFQQMIDTGLASLKFALIPPSLDQVFIGSMDLSRMYQVKCMFIIGVNDGVIPARPSDESVLSEDDREWLKRAGAELA
ETGKERLLDEQFLIYQALSSPSHHLYLSYAASDAEGRSLLPSPLIKYCQELMPNHQQALYVLDPELLEDDEQLKFVANEH
VSLSYTISQLQQWLNQYPISGVWWSVYNYLMTSPNRDVSKNIMSSLFFTNRAKPLKPNVTKELYGDHIQGSVSRMEKFNA
CAFSHFASHGLKLKDRQFYKLEAPDIGQLFHSALKHISDTLVEQKKDWKNLTKEDCVTYSRHAIEQLAPRLQKEILLSSN
RHAYIKEKLQKILIRVSSILSEHAKVSGFSPVGLELGFGGQGPLPPFTFQLKNGCTMELVGRIDRVDKAEGSKGLFLRIV
DYKSSEKGLDLAEVYYGLALQMLTYLDLTITYSKEWLGIEATPAGILYFHIHDPFIQAPIPLAEDEIEQEIFKKFKMKGL
LLEDVEAVKLMDQTLESGRSQVIQAGLKKDGSFRSDSAVLSEDHFHILTQHVRRTFEEAGERITNGEVAINPYKLKDQTP
CRFCSFKSICQFDESIEDNDFRVLTSEKDDVVIERIKKEGDQYANTKTE

Nucleotide


Download         Length: 3510 bp        

>NTDB_id=29264 BPUM_RS05115 WP_041815374.1 1073359..1076868(+) (addB) [Bacillus pumilus SAFR-032]
GTGGAGATTCAGTTTTTAGCAGGCAGATCGGGGAGTGGAAAAACGACTGCAATCTTAGAGGAAATCAAAGAACAGCTTCG
GCTTGATCCGTTGGGTCCGCCAATCATTTTTTTAGTCCCGGATCAAATGACATTTTTAATGGAATATGAGCTTGCGAAAA
CGTCTGAAGCTGGTGGAATGATCAGAGCTAAAGTATTTAGTTTCACTCGACTTGCTTGGTCTATTTTACAGCAGACTGGT
GGAGCGAACCGGCAATTTGTGACAAGCACAGGAATTCAAATGCTTTTAAGAAAAGTGATTGAAGAGCAGAAAGACAAGTT
TAAAGTATTCAAAAAAGCGAGTGATAAGCCGGGGTTTGTAGAGCAAATCGAAAAAACGATGGCTGAATTCAAAAGATACT
GTATGCTGCCTGAGGAAATTGAGAAAATTTCAGTGGAGAGCATGCTTTCAGAGTATACAGAAGAAAGACGGGCAGCCGAA
AAATTGCATGACCTCCATGTTCTTTATCAGCAGATGGAGGAGCATTTACAAGATGAATATGTGCACTCAGAAGACTATTT
GAATCTACTTGCCCAGCAAATTCCTTCAGCAGAAGAAATAAAAGGAGCACATATTTATATCGATGGTTTTTATCAATTTA
CACCGCAGCAGCTTCTTGTCATCGAACAGCTCTTGCTGCATGCAGCAAAAGTAACTGCGGCTTTTACAGTAGATCAATCT
TATCATGATAGGCAGCCAAATGAACTTGATTTATTTCGTATGACAGGTAAAACGTATTTCCAACTCTATCAGCTTGCTAA
AGAGTGCGGAGCTGACATTTCTGAAACCATTTTTGAAAGAAATCATCGGCACCTTTATACACCAGATCTTGCTTATTTAG
AACACCAATATGAGCAGCGGCCAGTACAACCATACCAGGAAAATACCCCTCATCTCACAGTGTCTAAAAGTGCAAGTAAA
CGAGCTGAAATTGAAGGAGTAGCGAGAGACATCCTTGATTTAGTGAGAGAAAAAGGACTTAGACTGCGAGATATCTCGGT
TGTAGCGCGGCATGTAGACGACTATAAAGATACGTTAAAAGAGGTTTTTCGAGATTACGATATTCCATTTTTTATTGATG
GAAATGAATCGATGCAGTATCATCCGCTCATCGAATTGATTCGTTCGAGCTTGGATGTCATAAAAGGAAATTGGCGGTAT
GAAGCGGTATTTCGCTGCGTAAAAACAGAGTTCTTATTTCCGCTTGAAATCACTAAGAACAAAGCACGAGAGCAGGCGGA
TCAGCTCGAAAACTATTGTATTGCTTACGGTGTAAAAGGAGAGCGCTGGACAAACGGTTCCCGGTTTCATTACAGGCGCT
TCCAATCATTAGATGAGGATTTCAGACAAACGGATCAAGAAATTGAAATGGAACAAATGCTGAATGACGTCAAAGAATGG
ATCACGCCTCCGCTTTACCAGTTGCAGAAAAGGCTCAAAAATGCGCAAAAGGTAAGAGATATGGTAGAGGCTGTCTATGT
CTTTTTAGAAGAGATACAAGTACCAGATAAGCTTGAAAAAGCGAGGCTAGAAGCTGAAGAAGCAGGTCGATTAGCTGAAG
CAATGCAGCACGGGCAAGTATGGGATGCGGTCATTCAATTAATGGATGAATTTGTTGACATGCTAGGTGACGAAGAGCTC
TCATTTCCTTTATTTCAACAGATGATAGATACAGGGTTAGCTTCTCTAAAATTCGCTTTAATTCCGCCATCACTCGACCA
AGTATTTATCGGAAGTATGGATTTATCCAGAATGTATCAAGTGAAGTGTATGTTTATCATTGGTGTAAATGATGGCGTTA
TTCCCGCGCGTCCCTCTGATGAGAGTGTATTGTCTGAAGATGACCGAGAATGGTTAAAGCGAGCAGGAGCAGAGCTGGCA
GAGACAGGAAAGGAACGGCTGCTAGATGAACAATTTTTAATTTACCAAGCGTTATCAAGTCCATCCCATCATTTATATCT
ATCCTATGCGGCTTCTGATGCAGAAGGACGTTCTCTATTGCCTTCGCCACTTATCAAGTATTGCCAAGAGCTTATGCCAA
ATCATCAGCAGGCTCTTTATGTGTTAGATCCAGAACTGCTGGAAGACGATGAGCAATTAAAATTTGTAGCGAATGAGCAT
GTTTCGTTGTCTTATACCATCTCACAGCTCCAGCAATGGCTCAATCAATATCCCATTAGCGGTGTATGGTGGAGTGTCTA
TAACTACTTAATGACATCTCCTAATCGGGATGTATCAAAAAATATCATGTCAAGTTTGTTCTTTACAAATCGAGCAAAGC
CATTAAAGCCTAATGTCACAAAAGAGCTTTACGGTGATCATATTCAGGGCAGTGTTTCAAGAATGGAGAAGTTCAATGCG
TGTGCATTTTCTCACTTTGCTTCCCACGGTTTAAAACTAAAGGATCGGCAATTTTACAAATTGGAAGCACCTGATATCGG
CCAGTTGTTTCACTCAGCTTTGAAGCATATTTCAGATACGCTAGTTGAACAAAAAAAGGATTGGAAAAACTTAACGAAGG
AAGATTGTGTCACCTATTCAAGACATGCAATCGAACAGCTTGCGCCGCGTCTTCAAAAGGAAATTCTATTAAGCTCTAAT
CGGCATGCTTATATTAAAGAAAAGCTTCAGAAAATCTTAATTCGAGTCTCTTCTATATTAAGTGAACATGCCAAAGTGAG
TGGATTTTCTCCAGTAGGGCTTGAGTTAGGTTTTGGAGGCCAAGGACCTTTGCCGCCGTTTACTTTTCAATTAAAGAATG
GCTGCACGATGGAGCTAGTCGGAAGAATTGACAGGGTAGATAAAGCAGAAGGTTCAAAAGGGCTGTTTTTAAGAATCGTC
GATTACAAATCCAGTGAGAAAGGTCTTGACCTAGCAGAAGTATACTATGGTCTTGCCTTGCAAATGCTCACATACTTAGA
CCTCACCATTACGTATTCGAAGGAGTGGCTAGGCATAGAAGCAACGCCTGCTGGCATTTTATATTTCCATATTCATGACC
CGTTCATTCAAGCCCCGATTCCACTTGCAGAGGATGAAATTGAGCAGGAAATATTCAAGAAATTTAAAATGAAGGGCTTA
TTACTTGAAGATGTGGAAGCAGTCAAGCTAATGGATCAAACGCTTGAGTCAGGTAGATCACAAGTCATTCAAGCCGGCTT
GAAAAAGGATGGGTCTTTCCGTTCTGATTCGGCGGTTTTAAGTGAAGATCATTTCCATATACTCACACAACACGTTCGGC
GCACATTTGAAGAAGCAGGCGAAAGAATTACAAATGGAGAGGTCGCAATTAATCCATATAAATTAAAGGACCAAACACCT
TGCCGCTTTTGTTCATTCAAGTCTATTTGCCAATTCGATGAATCAATAGAAGATAATGATTTTAGGGTGCTCACCTCTGA
AAAGGATGATGTTGTGATAGAACGGATCAAAAAAGAAGGGGATCAGTATGCAAATACCAAAACCGAATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

65.633

99.316

0.652

Multiple sequence alignment