Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   C3363_RS02400 Genome accession   NZ_CP029150
Coordinates   488419..489000 (-) Length   193 a.a.
NCBI ID   WP_010786056.1    Uniprot ID   A0A859IE21
Organism   Glaesserella parasuis strain GZ20170512     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 483419..494000
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C3363_RS02360 (C3363_02380) - 483761..484399 (+) 639 WP_010786061.1 nitroreductase family protein -
  C3363_RS02365 (C3363_02385) - 484488..485021 (+) 534 WP_010786060.1 TIGR00645 family protein -
  C3363_RS02370 (C3363_02390) - 485108..486406 (-) 1299 WP_010786059.1 NAD(P)/FAD-dependent oxidoreductase -
  C3363_RS02390 (C3363_02410) greB 486943..487422 (-) 480 WP_010786058.1 transcription elongation factor GreB -
  C3363_RS02395 (C3363_02415) - 487422..488357 (-) 936 WP_010786057.1 KpsF/GutQ family sugar-phosphate isomerase -
  C3363_RS02400 (C3363_02420) pilF 488419..489000 (-) 582 WP_010786056.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  C3363_RS02405 (C3363_02425) - 489247..490220 (+) 974 Protein_455 IS110 family transposase -
  C3363_RS02410 (C3363_02430) - 490264..491424 (-) 1161 WP_010786053.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  C3363_RS02415 (C3363_02435) - 491625..491933 (-) 309 WP_005710405.1 helix-turn-helix domain-containing protein -
  C3363_RS02420 (C3363_02440) - 491937..492275 (-) 339 WP_010786052.1 hypothetical protein -

Sequence

Protein


Download         Length: 193 a.a.        Molecular weight: 22339.29 Da        Isoelectric Point: 8.4221

>NTDB_id=290753 C3363_RS02400 WP_010786056.1 488419..489000(-) (pilF) [Glaesserella parasuis strain GZ20170512]
MTFAKFFRNLTACSVALWLVACTNQTAKVDFNRSEAVKARINLALAYLEQSDFPKAKENIDKALEHDVKDYLPHSVLAYY
YQQTGDNQKAEEAYQQALKLSKTQSKNNQVRPDVLNNYGTFLCKQKQFDKAYQQFETALTSQEAYYNQADTLENIALCAN
MKKDIEKQKTALSQLEKIDKSRAERLYNFMEIR

Nucleotide


Download         Length: 582 bp        

>NTDB_id=290753 C3363_RS02400 WP_010786056.1 488419..489000(-) (pilF) [Glaesserella parasuis strain GZ20170512]
ATGACCTTCGCAAAATTTTTCCGTAATTTGACCGCTTGTAGCGTGGCGTTATGGCTTGTTGCTTGTACAAATCAAACCGC
AAAGGTGGATTTTAACCGTTCAGAAGCCGTCAAAGCTCGTATTAATTTGGCTCTTGCTTACCTTGAACAATCTGATTTTC
CAAAAGCGAAAGAGAACATTGACAAAGCGCTTGAACATGATGTAAAAGACTACCTACCGCACTCTGTGCTTGCCTATTAT
TACCAACAAACTGGCGATAACCAAAAAGCAGAAGAAGCCTACCAGCAAGCCTTGAAGTTAAGTAAAACACAGAGCAAAAA
CAATCAAGTTCGCCCTGATGTATTGAATAACTACGGTACCTTTCTGTGCAAACAAAAACAGTTCGACAAAGCCTATCAGC
AATTTGAAACGGCATTAACCAGTCAAGAAGCCTATTACAATCAAGCAGATACACTAGAAAATATCGCCTTATGTGCCAAT
ATGAAGAAGGATATTGAGAAGCAAAAAACAGCACTATCTCAACTTGAGAAAATAGATAAATCAAGAGCAGAACGACTATA
TAATTTCATGGAGATTAGATAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_2)

(74-102)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A859IE21

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

97.927

100

0.979
  pilF/pilF2 Haemophilus influenzae Rd KW20

45.251

92.746

0.42

Multiple sequence alignment